Hexavalent Chromium (VI) Removal by Penicillium sp. IA-01

Abstract

The objective of this work was to study the removal of chromium VI in aqueous solution by the fungus Penicillium sp. IA-, isolated from polluted air with industrial vapors. To obtain the fungal biomass, pre-inoculums were performed in thioglycolate broth from a strain isolated from vapours contaminated with Cr VI . The fungus was incubated for four weeks at ambient temperature, filtered, and washed three times with trideionized water. In preparing cellullar fractions, it was necessary to break the fungal cells with glass beads using a homogenizer being careful to keep the samples in frosty cold ice. To obtain the fungal biomass, the fungus was filtered and stored in an oven at °C, allowing it to dry for h. Removal of Cr VI in vitro was evaluated using different cellular fractions and dead fungal biomass. We determine the optimal characteristics for metal removal in the reaction mixture. Concluding that the ideal conditions for the removal of Cr VI in the cell extracts were °C and pH . , also we observ that the highest enzyme activity was in the mixed membrane fraction. In dead fungal biomass, the ideal conditions for removal of metal are °C and pH . .

Bioremoval of Cobalt(II) from Aqueous Solution by Three Different and Resistant Fungal Biomasses

“…There are slightly more advanced studies with cellular fractions of the fungus Penicillium sp. IA-01 that indicate that the optimum pH of reduction and that of removal of Cr(VI) is 7.0, unlike the inert biomass in this study [24]. This phenomenon can be explained on the basis of the less competition between positively charged H + and Co 2+ ions for the similar functional group.…”

“…It is suggested that the fungi growing at concentrations up to 500 mg/L are tolerant and/or resistant to the metal, which is similar to that reported by Acosta-Rodríguez et al [10]; it is similar for Candida albicans , which grew at 300 mg/L of Co(II), for eight species of Penicillium isolated from Brazilian soil (tolerance between 50 and 500 µ g/mL); for Pichia guilliermondii isolated from acidic mine water in Peru, with a resistance to 400–600 mM of Co(II) [23]; and for the bacterium Pseudomonas aeruginosa SPB-1 (2.5 mM) [16], similar to the environmental-contaminant fungus Penicillium sp. IA-01 and which grew at a concentration of 500 ppm of Cr(VI) in an area near the Faculty of Chemical Sciences in San Luis Potosi, México, under the same conditions [24]. The variation in the metal tolerance might be due to the unique strategies or resistance/tolerance mechanisms exhibited by the microorganisms.…”

Biosorption of Chromium (VI) in Aqueous Solution by Ananas comosus Biomass Shell

“…This was due to the increase in the number of ions competing for the available functional groups on the surface of biomass [1,4]. On the other hand, at 60 °C, the biomass studied, shows the same results for removal, adsorbing 100% between 30 and 35 minutes with 200 and 1000mg/L of metal, respectively (Figure 4) with respect to other biomasses, this results are like for Penicillium sp., IA-01 [23], but there are other studies, which report a major biosorption with low concentrations: Citrus reticulata and Tamarindus indica shell [24,16]. In addition, we observe the development of a blue-green and white precipitate, which changes more rapidly at higher temperatures (date not shown).…”

“…In the experiment control (without biomass), the metal concentration of the water and earth samples decreased by about of 6% and 18% (date not shown); which can be caused by indigenous microflora and (or) reducing components present in the samples [1,4,8&29]. The chromium removal abilities of this biomass are equal or better than other biomasses reported, for example A. caudatus [13], Melaleuca diosmifolia leaf [28], T. indica, M. paradisiaca, C. limonium, and C. sinensis shells [30], maize leaf [31], C. melo shell [9], sawdust of pine tree [16], M. indica, M. paradisiaca, C. paradise, C. melo y C. máxima shells [24].…”

Biosorption of Hexavalent Chromium by Pseudomonas aeruginosa Strain ANSC: Equilibria Isothermic, Kinetic and Thermodynamic Studies

“…The suspended biomass was treated with 0.5% v/w triton X-100, 0.5% v/w tween 80, 0.5% v/w toluene, 0.5% w/w sodium deoxycholate and 0.5% w/w sodium dodecyl sulphate, and vortexed for 30 min to achieve cell permeabilisation. Permeabilised cell suspensions (2 wet g) were then added into 20mL of 100µg/mL solutions of K 2 Cr 2 O 7 , and incubated for 5 h at 37°C [6]. The samples were filtered.…”