Hexavalent chromium removal in vitro and from industrial wastes, using chromate-resistant strains of filamentous fungi indigenous to contaminated wastes

Aguilar, Francisco Javier Acevedo; Espino-Saldaña, Ángeles Edith; Leon-Rodriguez, Ireri Lizzuli; Rivera-Cano, M E; Ávila-Rodríguez, Mario; Wróbel, Kazimierz; Wróbel, Katarzyna; Lappe, Patricia; Ulloa, Miguel; Gutiérrez-Corona, J. Félix

doi:10.1139/w06-037

Cited by 54 publications

(35 citation statements)

References 20 publications

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“…These chromium uptake values are insignificant when compared with the initial Cr concentration in the culture medium. Similar observations on Cr uptake by fungal biomass of Aspergillus (strain Ed8) and Penicillium (strain H13) were reported by Acevedo-Aguilar et al [26] leading to suggestion that Cr uptake by these fungal strains was almost negligible, as compared to the total Cr present in the growth medium. They reported a total Cr uptake of only about 0.06% and 0.04% by the fungal biomass of the Aspergillus sp.…”

Section: Chromium Removal Via Uptake By the Fungal Biomasssupporting

confidence: 86%

“…The removal of chromate from the culture solutions was determined by estimating the residual Cr(VI) in the culture media after the 96 hour growth period as previously described by Shugaba et al [21][22][23] Cr(VI) uptake and reduction during growth was followed by loss of the yellow to orange colour of the dichromate solution and by the quantitative decrease in Cr(VI) concentration in culture [6,24,25] also, as part of the Cr(VI) reduction studies, the concentrations of trivalent chromium, [Cr(III)], and total chromium, Cr T were also determined in the culture medium [26] Samples of the spent culture solutions were filtered using a membrane filter before measuring the Cr(VI), Cr(III) and Cr T concentrations.…”

Section: Determination Of Cr(vi) Removal In the Fungal Culturementioning

confidence: 99%

“…Cr(VI) -resistant organisms are known to produce chromium reductase, which mediates the transfer of electrons from NAD(P)H to Cr(VI), converting it to Cr(III) [25,29,51]. The abilities of Aspergillus and Penicillium species to massively reduce Cr(VI) to Cr(III) have earlier been reported [26,[52][53][54][55].…”

Section: Cr(vi) -Reducing Activity Of Fungal Extractsmentioning

confidence: 99%

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Uptake and Reduction of Hexavalent Chromium by Aspergillus Niger and Aspergillus Parasiticus

Shugaba¹,

Buba²,

Bg³

et al. 2012

J Pet Environ Biotechnol

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The uptake and reduction of Cr(VI) by Aspergillus niger and A. parasiticus was studied in this journal. After 96 hours of growth, the culture solutions spiked with an initial dichromate concentration of 20 mg/l, were completely decolorized and had residual Cr(VI) concentrations of only 0.74 ± 0.55 and 1.69 ± 0.29 mg/l in A. niger and A. parasiticus cultures representing Cr(VI) removal of 96.3% and 91.6%, respectively. In the A. niger culture, significantly (P < 0.01) lower Cr(VI) concentrations were observed within 72 hours of growth compared to those of A. parasiticus, but in both cultures complete removal was almost achieved by 144 hours of growth. The rate of Cr(VI) removal was 0.21 ± 0.09 mgl -1 hr -1 and 0.20 ± 0.07 mgl -1 hr -1 for A. niger and A. parasiticus, respectively. Cellular concentrations of Cr(VI) in the two fungi increased significantly (P < 0.05 -0.001) with increasing concentrations of the dichromate treatments. Although tannic acid as sole source of carbon and energy gave significantly lower Cr(VI) removal than glucose (P < 0.001) and acetate (P < 0.01), it supported the removal of about 85.0% and 68.8% of the metal ion by A. niger and A. parasiticus, respectively. The active mycelia of both fungi showed significantly (P < 0.001) higher Cr(VI) removal than inactivated mycelia after incubation at 30°C for 72 hours. Incubation of cell -free extracts of both fungi with NADH at 30°C for 2 hours showed Cr(VI) reduction of 68.0% and 55.5% for A. niger and A. parasiticus, respectively. These findings suggest that uptake and metabolic reduction may be the process by which the two fungi are able to tolerate the toxic effects of hexavalent chromium. However, Cr removal via uptake by the two fungal biomass was observed to be in the range of 0.5 -1.78% only, for all the concentrations applied, which is insignificant when compared with the initial Cr concentration in the culture medium. The results obtained through this investigation indicate the possibility of treating waste effluents containing hexavalent chromium using Aspergillus niger and A. parasiticus.

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Section: Chromium Removal Via Uptake By the Fungal Biomasssupporting

confidence: 86%

Section: Determination Of Cr(vi) Removal In the Fungal Culturementioning

confidence: 99%

Section: Cr(vi) -Reducing Activity Of Fungal Extractsmentioning

confidence: 99%

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Uptake and Reduction of Hexavalent Chromium by Aspergillus Niger and Aspergillus Parasiticus

Shugaba¹,

Buba²,

Bg³

et al. 2012

J Pet Environ Biotechnol

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show abstract

“…N3 was superior to the other strains because it has the capacity for efficient chromium reduction under acidic conditions. Most other Cr(VI) reduction studies were carried out at neutral pH (Acevedo- Aguilar et al, 2006;Fude et al, 1994;Laxman and More, 2002;Mclean and Beveridge, 2001;RamirezRamirez et al, 2004). Aspergillus niger also has the ability to reduce and adsorb Cr(VI) (Srivastava and Thakur, 2006).…”

Section: Resultsmentioning

confidence: 99%

Cr(VI) reduction from contaminated soils by Aspergillus sp. N2 and Penicillium sp. N3 isolated from chromium deposits

Fukuda

Ishino

Ogawa

et al. 2008

J. Gen. Appl. Microbiol.

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“…Estos mecanismos se aplican en los ciclos biogeoquímicos en los cuales aparecen variantes microbianas capaces de tolerar los efectos nocivos de los metales, de allí surgen los microorganismos resistentes, caracterizados por poseer mecanismos de detoxificación codificados genéticamente e inducibles por el metal, y los tolerantes desarrollan indiferentes a la presencia del metal (6). Los microorganismos más estudiados en procesos de remediación son las bacterias debido que reducen el Cr(VI) tóxico a Cr(III) menos tóxico (7,8) y menos estudiados son los hongos filamentosos (9,10). De acuerdo a la bibliografía, las células fúngicas interaccionan con el Cr (VI) y Cr(III) a diferentes niveles (Figura 1), desde la pared celular, el periplasma y la membrana plasmática, hasta el citoplasma y las organelas celulares (11).…”

Section: Introduccionunclassified

REMEDIACION DE RESIDUOS SÓLIDOS CONTAMINADOS CON Cr(VI) POR UN HONGO FILAMENTOSO

Busnelli

et al. 2015

Bol. Micol.

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RESUMENLa biotecnología ambiental recurre a organismos capaces de reducir los niveles de metales pesados, entre ellos el Cr(VI), contenido en residuos y efluentes agroindustriales. El objetivo del trabajo fue estudiar la biorremediación de un residuo como pulpa de limón contaminada con Cr(VI) y el efecto del metal sobre el crecimiento fúngico. Se utilizaron tres hongos filamentosos como Aspergillus niger; Penicillium expansum y P. islandicum para remediar pulpa de limón (residuo) contaminada con Cr(VI) (50 mg/L) que se realizó en las siguientes condiciones de cultivo: la pulpa se suplementó con urea, 0,006; (NH 4 ) 2 SO 4 , 0,012; KH 2 PO 4 , 0,003 y KCl, 0,001 g/ g; 10 5 conidios/ g, a pH 2,5, 30°C y 96 h de incubación. Se estudió el efecto tóxico de diferentes concentraciones (5; 10; 20 y 50 mg/L) del metal sobre el desarrollo del hongo de mayor eficiencia de remediación (Ef.%). Aspergillus niger; obtuvo mayor EF. de remediación (97%) respecto a Penicillium expansum (95%) y P. islandicum (94%), del residuo contaminado con 50 mg/L de Cr(VI). Se determinó que la presencia de Cr(VI) y no su concentración estimuló la maduración temprana (48 h) de los conidios (blancos a negros) de A. niger, sin que se observe alteraciones en el micelio con respecto al control (72h), desarrollado en la pulpa sin el metal. En conclusión, A. niger fue más resistente y presentó altas Ef. de remediación de Cr(VI) de residuos sólidos, este proceso es una alternativa a las tecnologías físico-químicas, debido que los microorganismos pueden remover selectivamente diferentes iones de zonas contaminadas. ABSTRACTEnvironmental biotechnology uses organisms capable of reducing levels of heavy metals, including the Cr (VI), contained in waste and agro-industrial effluents. The objective of this work was to study bioremediation of waste contaminated with Cr(VI) lemon pulp and the effect of the metal on the fungal growth. We used three filamentous fungi such as Aspergillus niger; Penicillium expansum and P. islandicum to remedy pulp from lemon (residue) contaminated with Cr(VI) (50 mg/L) that was conducted in the following conditions of cultivation: the pulp is supplemented with urea, 0.006; (NH4) 2 SO 4 , 0.012; KH 2 PO 4 , 0.003 and KCl, 0.001 g / g; 10 5 conidia/g, at pH 2.5, 30 ° C and 96 h of incubation. We studied the toxic effect of different concentrations (5, 10, 20 and 50 mg/L) of the metal on the Bol. Micol. 2015; 30(1): 2 -9 MICOLOGÍA AMBIENTAL micologia.uv.cl

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Hexavalent chromium removal in vitro and from industrial wastes, using chromate-resistant strains of filamentous fungi indigenous to contaminated wastes

Cited by 54 publications

References 20 publications

Uptake and Reduction of Hexavalent Chromium by Aspergillus Niger and Aspergillus Parasiticus

Uptake and Reduction of Hexavalent Chromium by Aspergillus Niger and Aspergillus Parasiticus

Cr(VI) reduction from contaminated soils by Aspergillus sp. N2 and Penicillium sp. N3 isolated from chromium deposits

REMEDIACION DE RESIDUOS SÓLIDOS CONTAMINADOS CON Cr(VI) POR UN HONGO FILAMENTOSO

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