Hexavalent chromium disrupts the skin barrier by targeting ROS-mediated mitochondrial pathway apoptosis in keratinocytes

Wang, Cheng; Shang, Hongqi; Zhang, Shuyu; Wang, Xiangkun; Liu, Defeng; Shen, Mingyue; Li, Ning; Jiang, Yunxuan; Wei, Kai; Zhu, Rui‐Liang

doi:10.1016/j.cbi.2023.110523

Cited by 5 publications

(3 citation statements)

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“…We then confirmed our suspicions experimentally and found that intracellular reactive oxygen species and calcium ion levels increased significantly after the cowshed PM 2.5 stimulation, which might be one of the reasons for the induction of apoptosis. As the mitochondrial apoptotic pathway is one of the main pathways through which apoptosis occurs [53], the accumulation of reactive oxygen species led us to associate it with the mitochondrial apoptotic pathway [54], as evidenced by the decrease in mitochondrial membrane potential and proteins such as cytochrome C, which is also consistent with previous experiments. Subsequently, a change in expression of miR-212-5p was found, and its target gene ARAF was confirmed using a dual-luciferase assay.…”

Section: Discussionsupporting

confidence: 86%

MicroRNA miR-212-5p Regulates the MEK/ERK Signaling Pathway by Targeting A-Raf proto-oncogene serine/threonine-protein kinase (ARAF) to Regulate Cowshed PM2.5-Induced NR8383 Apoptosis

Sun,

Jia

et al. 2023

Toxics

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Objective: To investigate the role of miR-212-5p-targeted ARAF during the apoptosis of rat alveolar macrophages induced by cowshed PM2.5. Methods: miRNA and related target genes and pathways were predicted using the KEGG, TargetScan, and other prediction websites. NR8383 macrophages were treated with cowshed PM2.5 to establish an in vitro lung injury model in rats; meanwhile, for the assessment of cell viability, apoptosis, intracellular calcium ions, and mitochondrial membrane potential in NR8383 cells, RT-qPCR was used to detect the expression of miR-212-5p and the target gene ARAF. Results: The bioinformatic analyses showed that miR-212-5p and ARAF were involved in PM2.5-associated cellular damage. Exposure to different concentrations (0 μg/mL, 60 μg/mL, 180 μg/mL, 300 μg/mL) with different durations (0 h, 12 h, 24 h, 48 h) of cowshed PM2.5 resulted in apoptosis, increased intracellular calcium ions, and decreased mitochondrial membrane potential. The miR-212-5p mimic group showed an up-regulation of Bax and cleaved Caspase 3 expression but decreased Bcl2 expression compared to the NC group, and overexpression of ARAF up-regulated the expression of p-MEK1/2 and p-ERK1/2 and simultaneously reversed the above phenomena. Conclusions: miR-212-5p targets ARAF to affect the cowshed PM2.5-induced apoptosis through the MEK/ERK signaling pathway, providing a potential target for relevant farming industry and pathology studies.

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Section: Discussionsupporting

confidence: 86%

MicroRNA miR-212-5p Regulates the MEK/ERK Signaling Pathway by Targeting A-Raf proto-oncogene serine/threonine-protein kinase (ARAF) to Regulate Cowshed PM2.5-Induced NR8383 Apoptosis

Sun,

Jia

et al. 2023

Toxics

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“…1,2 Excessive Cr( vi ) pollution in aquatic systems can increase the threat of poisoning, respiratory diseases, and skin cancer. 3,4 Thus far, several techniques have been developed to remediate Cr( vi )-polluted water, such as ion exchange, catalysis, adsorption, and electrochemical treatment. 5–8 Among these methods, photocatalytic Cr( vi ) reduction is considered one of the most favored approaches 9 and possesses the benefits of low cost, high efficiency, and no detrimental byproduct formation.…”

Section: Introductionmentioning

confidence: 99%

A series of four-substituted polyoxometalate-thiacalix[4]arene-based materials for the efficient photocatalytic reduction of Cr(vi)

Zhang,

Song,

Yang

et al. 2024

Inorg. Chem. Front.

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“…The skin, situated in the outermost layer of the human structure, holds the distinction of being the largest organ in the human body ( Wang et al, 2023a ; Yang et al, 2023a ). It primarily serves as an immune defense and protective barrier against external pathogens and harmful substances while regulating the body’s metabolism and facilitating sensory perception.…”

Section: Introductionmentioning

confidence: 99%

Advancements in engineered exosomes for wound repair: current research and future perspectives

Ye,

Wang,

et al. 2023

Front. Bioeng. Biotechnol.

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Wound healing is a complex and prolonged process that remains a significant challenge in clinical practice. Exosomes, a type of nanoscale extracellular vesicles naturally secreted by cells, are endowed with numerous advantageous attributes, including superior biocompatibility, minimal toxicity, and non-specific immunogenicity. These properties render them an exceptionally promising candidate for bioengineering applications. Recent advances have illustrated the potential of exosome therapy in promoting tissue repair. To further augment their therapeutic efficacy, the concept of engineered exosomes has been proposed. These are designed and functionally modifiable exosomes that have been tailored on the attributes of natural exosomes. This comprehensive review delineates various strategies for exosome engineering, placing specific emphasis on studies exploring the application of engineered exosomes for precision therapy in wound healing. Furthermore, this review sheds light on strategies for integrating exosomes with biomaterials to enhance delivery effectiveness. The insights presented herein provide novel perspectives and lay a robust foundation for forthcoming research in the realm of cutaneous wound repair therapies.

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Hexavalent chromium disrupts the skin barrier by targeting ROS-mediated mitochondrial pathway apoptosis in keratinocytes

Cited by 5 publications

References 50 publications

MicroRNA miR-212-5p Regulates the MEK/ERK Signaling Pathway by Targeting A-Raf proto-oncogene serine/threonine-protein kinase (ARAF) to Regulate Cowshed PM2.5-Induced NR8383 Apoptosis

MicroRNA miR-212-5p Regulates the MEK/ERK Signaling Pathway by Targeting A-Raf proto-oncogene serine/threonine-protein kinase (ARAF) to Regulate Cowshed PM2.5-Induced NR8383 Apoptosis

A series of four-substituted polyoxometalate-thiacalix[4]arene-based materials for the efficient photocatalytic reduction of Cr(vi)

Advancements in engineered exosomes for wound repair: current research and future perspectives

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