Hexamethylendiisocyanat, 2,4‐Toluylendiisocyanat, 2,6‐Toluylendiisocyanat, Isophorondiisocyanat und Diphenylmethan‐4,4′‐diisocyanat – Bestimmung von Hexamethylendiamin, 2,4‐Toluylendiamin, 2,6‐Toluylendiamin, Isophorondiamin und 4,4′‐Diaminodiphenylmethan in Urin mittels Gaschromatographie‐Massenspektrometrie [Biomonitoring Methods in German language, 2017]

Abstract

The working group “Analyses in Biological Materials” of the Permanent Senate Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area verified the present biomonitoring method. The described method allows the simultaneous determination of the metabolites of hexamethylene diisocyanate ( HDI ), 2,4‐toluenediamine and 2,6‐toluene diisocyanate ( TDI ), isophorone diisocyanate ( IPDI ) and methylene diphenyl diisocyanate (MDI) in human urine. After acid hydrolysis the released amines, hexamethylenediamine ( HDA ), 2,4‐, and 2,6‐toluenediamine ( TDA ), isophorone diamine ( IPDA ) and 4,4′‐methylenedianiline ( 4,4 ′ ‐MDA ) are extracted from urine, derivatised using heptafluorobutyric anhydride and quantified by NCI‐GC‐MS. The method was extensively validated and the reliability data were confirmed by independent laboratories, which have established and cross‐checked the whole procedure