Hexahydrocurcumin alleviated blood–brain barrier dysfunction in cerebral ischemia/reperfusion rats

Wicha, Piyawadee; Tocharus, Jiraporn; Janyou, Adchara; Jittiwat, Jinatta; Chaichompoo, Waraluck; Suksamrarn, Apichart; Tocharus, Chainarong

doi:10.1007/s43440-019-00050-9

Cited by 22 publications

(14 citation statements)

References 47 publications

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“…Several other studies have documented that HHC showed vasorelaxant effects [26], anti-angiogenic effects [27], and antiatherosclerogenic effects by inhibiting platelet aggregation [28]. Furthermore, HHC alleviated brain damage in rats after cerebral ischemia/reperfusion injury [29]. OHC is the final hydrogenated metabolite (HM) of curcumin and has been shown to exert better antitumor efficacy than curcumin [30].…”

Section: Introductionmentioning

confidence: 99%

Comparative Study of Curcumin and Its Hydrogenated Metabolites, Tetrahydrocurcumin, Hexahydrocurcumin, and Octahydrocurcumin, on Melanogenesis in B16F10 and MNT-1 Cells

Goenka

Simon

2021

Cosmetics

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Curcumin, a bioactive from Curcuma longa, has been shown to possess anti-melanogenic activity previously; however, the effects of its hydrogenated metabolites (HMs)—Tetrahydrocurcumin (THC), Hexahydrocurcumin (HHC), and Octahydrocurcumin (OHC)—on melanogenesis have not been sufficiently explored. We have studied and compared three HMs (THC, HHC, and OHC) with the parent compound, curcumin (PC), on melanin synthesis in B16F10 mouse and MNT-1 human melanoma cells. Our results demonstrated that all the HMs were nontoxic over the concentration range 5–40 µM, while PC was nontoxic at 5 µM but induced toxicity at 20 and 40 µM in B16F10 cells. All three HMs enhanced melanin synthesis, while PC (5 µM) inhibited it. THC (40 µM) significantly stimulated melanin synthesis to a greater degree than HHC and OHC in both B16F10 and MNT-1 cells; the order of melanogenesis stimulation was THC = OHC > HHC in B16F10 mouse cells, while it was THC > HHC > OHC in MNT-1 cells. HMs stimulated melanogenesis by pathways not involving tyrosinase, as neither the intracellular tyrosinase activity nor the protein levels of tyrosinase were affected. In addition, mushroom tyrosinase activity, using L-Dihydroxyphenylalanine (L-DOPA) as the substrate, showed no direct effects of HMs. In summary, our results demonstrate that the HMs enhanced melanogenesis, which establishes that the hydrogenation of the heptadiene moiety of curcumin leads to a loss of its anti-melanogenic activity and instead results in the stimulation of melanogenesis. This stimulation is not further enhanced upon hydrogenation of the β-diketone, which was noted in MNT-1 cells, although the correlation to the number of keto groups differed in B16F10 cells where HHC was the weakest stimulator of melanogenesis. Collectively, THC with both keto groups intact is the best stimulator. Moreover, our results also validate that the electrophilicity of curcumin is necessary for its anti-melanogenic activity, as the non-electrophilic HMs did not inhibit melanogenesis. Furthermore, our results suggest that THC might hold promise as a stimulator of melanogenesis for treatment of hypopigmentation disorders and anti-graying therapies. Future studies to probe the molecular signaling mechanisms and test whether the pro-melanogenic activity of HMs is retained in primary human melanocytes are warranted.

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Section: Introductionmentioning

confidence: 99%

Comparative Study of Curcumin and Its Hydrogenated Metabolites, Tetrahydrocurcumin, Hexahydrocurcumin, and Octahydrocurcumin, on Melanogenesis in B16F10 and MNT-1 Cells

Goenka

Simon

2021

Cosmetics

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“…For the validation of the method, parameters such as sensitivity, limit, accuracy, precision, and repeatability have been assessed using quantification of Immunoglobulin (IgG) infiltration in a stroke mouse model. Level of IgG extravasation into the brain and related morphological changes are important indices to judge the degree of blood-brain barrier damage during cerebral ischemia [7][8][9] and there are no methods currently available to do both in one intact tissue block at the same time. However, with the Histo-ELISA, we quantified IgG infiltration and observed pathological changes spontaneously and by the way, the effectiveness of revacept on the stroke was evaluated in the ischemia models.…”

Section: Histo-elisa Technique For Quantification and Localization Ofmentioning

confidence: 99%

Histo-ELISA technique for quantification and localization of tissue components

Goebel

Reimann

et al. 2020

Sci Rep

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A novel Histo-ELISA technique is intended to facilitate quantification of target tissue proteins in a tissue section and involves the selection of target regions in the tissue section, application of streptavidin-conjugated HRP (horseradish peroxidase), coupled with peroxidase substrate—TMB (3,3′,5,5′-tetramethylbenzidine), and staining dye evaluation with ELISA reader. The target protein content (weight per volume unit) was translated from optical densities by a reference standard curve, obtained via parallel staining of the targeted protein-coated slides. To validate the technique, we carried out quantifications of IgG extravasation in ischemic and nonischemic brain sections in a mouse stroke model. With those obtained data and the reference of immunohistochemistry scores assessed on the adjacent sections, accuracy, sensitivity, and precision for the technique were evaluated. For all evaluated parameters, Histo-ELISA performance was either comparable to or better than the standard immunohistochemistry. A comparison with the data from the repeated measurements yielded a rather low coefficient of variation. The results confirmed that the technique is a fairly reliable quantitative test with rather high sensitivity, accuracy, precision, and reproducibility for detecting target protein content in tissue sections and that its tissue distribution and related subsequent morphological changes can be observed at the same time.

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“…To measure the infarct volume, TTC staining was carried out as described previously [23]. Brie y, brain tissue was cut into ve coronal slices (2 mm thick) and incubated in 1% TTC dissolved in PBS for 15 min at 37 °C.…”

Section: Ttc Stainingmentioning

confidence: 99%

Transplanted Hair Follicle Stem Cells Migrate to the Penumbra and Express Neural Markers in a Rat Model of Cerebral Ischaemia/Reperfusion

Zhang

Tang

Mao

et al. 2020

Preprint

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Background: Ischaemic stroke has become the main cause of death and severe neurological disorders, for which effective restorative treatments are currently limited. While stem cell transplantation offers therapeutic potential through neural regeneration, this approach is associated with challenges of limited applicable sources. Hair follicle stem cells (HFSCs) are multipotential cells that can differentiate into ectodermal and mesodermal lineages and proliferate for long periods. The therapeutic potentials of HFSCs have not been investigated in ischaemic stroke models and, therefore, in this study, we aimed to determine whether they could survive and migrate to ischaemic areas after stroke attack. Methods : A rat model of middle cerebral artery ischaemia/reperfusion was established and intravenously administered HFSCs. The potential of HFSCs to migrate and differentiate into neuron-like cells as well as their ability to reduce the infarct size was evaluated. Rat brain tissue samples were collected 2 weeks after cell transplantation and analysed via TTC staining, immunofluorescence, and immunohistochemistry methods. The data were statistically analysed and presented as the means ± standard deviations. Results : Intravenously administrated rat HFSCs were able to migrate to the penumbra where they expressed neuron-specific markers, reduced the infarct volume, and promoted neurological recovery. Conclusion : HFSC transplantation has therapeutic potential for ischaemic stroke, and is, therefore, worthy of further investigation toward possible clinical development for treating stroke patients.

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Hexahydrocurcumin alleviated blood–brain barrier dysfunction in cerebral ischemia/reperfusion rats

Cited by 22 publications

References 47 publications

Comparative Study of Curcumin and Its Hydrogenated Metabolites, Tetrahydrocurcumin, Hexahydrocurcumin, and Octahydrocurcumin, on Melanogenesis in B16F10 and MNT-1 Cells

Comparative Study of Curcumin and Its Hydrogenated Metabolites, Tetrahydrocurcumin, Hexahydrocurcumin, and Octahydrocurcumin, on Melanogenesis in B16F10 and MNT-1 Cells

Histo-ELISA technique for quantification and localization of tissue components

Transplanted Hair Follicle Stem Cells Migrate to the Penumbra and Express Neural Markers in a Rat Model of Cerebral Ischaemia/Reperfusion

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