Hexadecylphosphocholine (Miltefosine) stabilized chitosan modified Ampholipospheres as prototype co-delivery vehicle for enhanced killing of L. donovani

Tripathi, Priyanka; Jaiswal, Anil K.; Dube, Anuradha; Mishra, Prabhat Ranjan

doi:10.1016/j.ijbiomac.2017.07.076

Cited by 22 publications

(46 citation statements)

References 38 publications

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“…The results of in vitro tests (promastigote and amastigote) showed that AK nanodrug was not very effective in parasite killing. However, they demonstrated an only negligible effect on inhibiting parasite in vivo environment, which is possibly due to a low dose of AK1 mg/kg (7- 10,12,13,15,16,24). In the present study, A was loaded into K synthesized by phase separation method (different from previous studies by using ionic gelation) and it was found that K decreased the toxicity effects of A and increased its solubility (14,18).…”

Section: Discussionmentioning

confidence: 55%

“…Current therapies used for leishmaniasis encountered some difficulties due to their high toxicity and low water solubility (8,10,(20)(21)(22)(23). Nowadays nanocarriers were successful in solving these problems (5).…”

Section: Discussionmentioning

confidence: 99%

“…Our findings revealed that parasites were significantly inhibited in our mice model. This success was resulted from increasing the effective therapeutic dose of A to 10 mg/kg and K properties including its antileishmanial, immunomodulatory, and wound healing effects (10,25).…”

Section: Discussionmentioning

confidence: 99%

“…Recently, nanotechnology was considered as an appropriate alternative due to the fact that we can deliver current medicines by nanosized carriers and improve bioavailability and reduce toxicity (5). In this regard, researchers have used two nanocarriers of nanochitosan (K) and anionic linear globular dendrimer (ALGD) for treatment of leishmaniasis to decrease the side effects of conventional medicines (6)(7)(8)(9)(10)(11)(12)(13). Nanochitosan is a nanocarrier with ability to decrease the drug toxicity and increase the drug solubility.…”

Section: Introductionmentioning

confidence: 99%

“…It has additionally immunomodulatory and wound healing effects and was synthesized with various methods including ionic gelation, phase separation, etc. (10,14). Some studies applied nanoformulation of amphotericin B-nanochitosan (AK) synthesized by ionic gelation on leishmaniasis treatment (7-10, 12, 13, 15, 16).…”

Section: Introductionmentioning

confidence: 99%

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In Vivo Therapeutic Effects of Four Synthesized Antileishmanial Nanodrugs in the Treatment of Leishmaniasis

Mehrizi

Mosaffa

Hoseini

et al. 2018

Arch Clin Infect Dis

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Background: Nowadays, nanocarriers are used for leishmaniasis treatment due to development of drug resistance and several side effects with conventional therapeutics. Objectives: In this study we aimed to evaluate in vivo effects of four synthesized nanodrugs including amphotericin B-nanochitosan (AK), betulinic acid-nanochitosan (BK), amphotericin B-dendrimer (AD), and betulinic acid-dendrimer (BD) in the treatment of Leishmania major infection (L. major) in mice model by using pathological analyses to choose the most effective nanodrug in leishmaniasis. Methods: The four nanodrugs efficacy in the improvement of L. major lesion in a mice model was evaluated by using pathological analyses including measurement of organs size and parasite number. Additionally, the nanodrugs toxicity was evaluated by measurement of various blood factors. Results: The histopathological results of the present study showed that BK, at the dose of 20 mg/kg, and AK, at the dose of 10 mg/kg, were more effective in decreasing the parasite number in the kidney, liver, and spleen. Moreover, BK20 mg/kg and AK10 mg/kg decreased the organs size significantly while AD50 mg/kg and BD40 mg/kg were less effective. However, none of the four nanodrugs had increased the blood factors and they were not toxic. Conclusions: Overall, the pathologic findings of various mice organs treated with different formulations showed that AK10 mg/kg and BK20 mg/kg were more effective in recovery of L. major's pathological effects in comparison to AD50 mg/kg and BD40 mg/kg. Therefore, it seems that AK and BK, in this mentioned dosage, could be considered as a proper candidate for treatment of leishmaniasis.

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