Hexacosanol reduces plasma and hepatic cholesterol by activation of AMP-activated protein kinase and suppression of sterol regulatory element-binding protein-2 in HepG2 and C57BL/6J mice

Lee, Ji Hae; Jia, Yongyi; Thach, Trung Thanh; Han, Yuri; Kim, Bobae; Wu, Chunyan; Kim, Yeonji; Seo, Woo Duck; Lee, Sung Joon

doi:10.1016/j.nutres.2017.05.013

Cited by 25 publications

(25 citation statements)

References 42 publications

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“…A recent paper reported that hexacosanol reduces plasma and hepatic cholesterol by activation of adenosine 5′-monophosphate- (AMP-) activated protein kinase (AMPK) and suppression of sterol regulatory element-binding protein-2 in HepG2 and C57BL/6J mice [ 36 ]. It has been well known that AMPK activation activities were correlated with increased export of cholesterol and excretion of cholesterol [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

Consumption of Cuban Policosanol Improves Blood Pressure and Lipid Profile via Enhancement of HDL Functionality in Healthy Women Subjects: Randomized, Double‐Blinded, and Placebo‐Controlled Study

Cho

Kim

Yadav

et al. 2018

Oxidative Medicine and Cellular Longevity

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Policosanol has been reported to improve blood pressure, lipid profile, and HDL functionality via inhibition of cholesteryl ester transfer protein (CETP) both in vitro and in vivo in zebrafish and human models. However, there are limited reports and randomized, double-blinded trials on policosanol that could advocate the blood pressure-lowering effect in prehypertensive participants. Therefore, we performed in vitro, in vivo, and ex vivo experiments to provide more substantial and concrete data on the blood pressure-lowering effect of policosanol. Consumption of policosanol for 8 weeks enhanced plasma antioxidant activity. In the policosanol group, plasma total cholesterol (TC) and triglyceride (TG) levels were reduced up to 20% and 14%, respectively, and HDL-C level was elevated up to 1.3-fold compared to that at week 0. TG/HDL-C and cholesteryl ester transfer protein (CETP) activities were reduced up to 36% and 20%, respectively. Uptake of oxidized LDL in macrophages was reduced as oxidized species levels were reduced, and HDL2-associated paraoxonase activities were enhanced by 60% compared to those at week 0. Encapsulation of policosanol into reconstituted HDL (PCO-rHDL) enhanced cholesterol efflux activity and insulin secretion capacity. In conclusion, consumption of policosanol for 8 weeks in healthy female subjects resulted in lowered blood pressure and CETP activity via elevation of HDL/apoA-I contents and enhancement of HDL functionalities, including cholesterol efflux and insulin secretion. These functional enhancements of HDL can contribute to the prevention of aging-related diseases, hypertension, and stroke.

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Section: Discussionmentioning

confidence: 99%

Consumption of Cuban Policosanol Improves Blood Pressure and Lipid Profile via Enhancement of HDL Functionality in Healthy Women Subjects: Randomized, Double‐Blinded, and Placebo‐Controlled Study

Cho

Kim

Yadav

et al. 2018

Oxidative Medicine and Cellular Longevity

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“…Cells were cultured using Dulbecco’s modified Eagle’s medium (Caisson, North Logan, UT, USA) supplemented with the 10% fetal bovine serum (Gendepot, Barker, TX, USA) and 1% penicillin/streptomycin (Caisson). Cells were grown in humidified 5% CO 2 incubator at 37°C (Lee et al, ).…”

Section: Methodsmentioning

confidence: 99%

“…The membranes were washed with TBS‐T for 40 min and then incubated with secondary antibody (Santa Cruz Biotechnology, Dallas, TX, USA) for another 1 hr at room temperature. Immunoreactive protein bands were detected with chemiluminescence system (GenDEPOT), visualized using a Sensi‐Q2000 image analyzer (Lugen Sci, Bucheon, Korea) and quantified with Gel‐Pro Analyzer software (Lee et al, ).…”

Section: Methodsmentioning

confidence: 99%

Oat germination and ultrafiltration process improves the polyphenol and avenanthramide contents with protective effect in oxidative‐damaged HepG2 cells

Lee

Park

et al. 2019

J Food Biochem

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Oat is the nutritious crop containing various compounds with antioxidant properties, such as polyphenols. In this study, we investigated the effect of germination and ultrafiltration process on polyphenol and avenanthramide contents in oat as well as their cytoprotective effect. Germination of oat for 48 hr significantly increased avenanthramide (5.5 to 11.3 mg/g) and polyphenol (115 to 155 mg GAE/g) contents. The compounds were more concentrated after ultrafiltration using 10 kDa membranes (polyphenol, 206 GAE/g; avenanthramide, 18 mg/g). In addition, oat extracts significantly reduced the cellular ROS level against tert‐butyl hydroperoxide (t‐BHP) stimulation in HepG2 cells. In the mechanistic study, oat extracts induced Nrf2 translocation to the nucleus by inhibition of Keap1 expression, resulting into upregulation of γ‐GCS and NQO1. In conclusion, oat germination and ultrafiltration processes increased the polyphenol content, including that of avenanthramide. These extracts protected cells from t‐BHP by radical scavenging activities and induced Nrf2 pathway activation. Practical applications This study presents the method for avenanthramide‐concentrated extract which is unique bioactive compounds in oat. In addition, antioxidant activity and their mechanisms of the avenanthramide‐enriched extracts were evaluated. The polyphenol compounds including avenanthramide were found to increase after germination and ultrafiltration, thereby improving the radical scavenging ability. These results can be utilized as data for the development of health‐promoting materials using oats.

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“…Numerous beneficial properties have been reported, both to human health and as a growth promoter in plants and cyanobacteria, for POL derived from different waxy sources [1][2][3]. Pharmacological studies have been performed with mixtures of LCAs that form the POL mix; however, every component shows special characteristics that are beneficial for different diseases and disorders of human health [4][5][6]. Some of these compounds, such as Triacontanol (TRIA), Octacosanol (OCTA), and Hexacosanol (HEXA), have been evaluated in in vitro and in vivo systems [5,6], and multiple efforts have been made to determine their mechanisms of action, metabolism, and interactions with other drugs [4,5].…”

Section: Introductionmentioning

confidence: 99%

Main Mechanisms of Action of Policosanol in Animal and Plant Cells

Romero-Martínez¹,

Ramos-Zambrano²,

Osorio-Ruiz³

et al. 2021

Int j pharm res allied sci

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Policosanol is a promising compound that can be used in various industries. In the pharmaceutical industry, policosanol and, specifically, triacontanol, octacosanol, and hexacosanol have presented biological activity against several diseases, especially those related to inflammation and hypercholesterolaemia. On the other hand, triacontanol is used as a growth promoter in a wide variety of plants and crops of economic importance, even in microalgal culture, in either its pure form or in policosanol extracts. In this review, the most relevant references to the bioactivities of policosanol in plants and animal cells are collected to compare the different mechanisms of action. Analyzing this in detail allows us to ask new research questions. The PubMed and Redalyc database were used for article search under the following keys terms: policosanol, inflammatory mechanisms, triacontanol, cellular absorption, photosynthesis and photoinhibition. Policosanol interferes with the progression of the NF-κB and MAPK signaling pathway involved in the inflammation process. The cholesterol-lowering effect of policosanol is due to the inhibition of cholesterol synthesis in the liver, through the indirect inactivation of HMG-CoA reductase. Triacontanol improves the growth and the biochemical and physiological parameters of plants and the response to stress conditions that is related mainly to photosynthetic activity. Interestingly, octacosanol demonstrates an inhibitory activity on the effect of triacontanol in plants, a response not reported in human cells, as well as other differential aspects of the mechanisms of action in the cells of plants and animals that are interesting to analyze.

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Hexacosanol reduces plasma and hepatic cholesterol by activation of AMP-activated protein kinase and suppression of sterol regulatory element-binding protein-2 in HepG2 and C57BL/6J mice

Cited by 25 publications

References 42 publications

Consumption of Cuban Policosanol Improves Blood Pressure and Lipid Profile via Enhancement of HDL Functionality in Healthy Women Subjects: Randomized, Double‐Blinded, and Placebo‐Controlled Study

Consumption of Cuban Policosanol Improves Blood Pressure and Lipid Profile via Enhancement of HDL Functionality in Healthy Women Subjects: Randomized, Double‐Blinded, and Placebo‐Controlled Study

Oat germination and ultrafiltration process improves the polyphenol and avenanthramide contents with protective effect in oxidative‐damaged HepG2 cells

Main Mechanisms of Action of Policosanol in Animal and Plant Cells

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