Heterotrimeric G-Protein γ Subunit CsGG3.2 Positively Regulates the Expression of CBF Genes and Chilling Tolerance in Cucumber

Bai, Longqiang; Liu, Yumei; Mu, Ying; Anwar, Ali; He, Chunguang; Yan, Yan; Li, Yansu; Yu, Xianchang

doi:10.3389/fpls.2018.00488

Cited by 35 publications

(22 citation statements)

References 48 publications

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“…Through several studies, various stress tolerance genes that have profound effects on enhancing drought tolerance in plants have been identified, and among them are the members of GPCR genes. GPCRs play a crucial role in regulating plant water status under normal and adverse circumstances (Bai et al, 2018; Lu et al, 2018; Wong et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Induced Expression of Xerophyta viscosa XvSap1 Gene Enhances Drought Tolerance in Transgenic Sweet Potato

2019

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Drought stress often leads to reduced yields and is a perilous delimiter for expanded cultivation and increased productivity of sweet potato. Cell wall stabilization proteins have been identified to play a pivotal role in mechanical stabilization during desiccation stress mitigation in plants. They are involved in numerous cellular processes that modify cell wall properties to tolerate the mechanical stress during dehydration. This provides a plausible approach to engineer crops for enhanced stable yields under adverse climatic conditions. In this study, we genetically engineered sweet potato cv. Jewel with XvSap1 gene encoding a protein related to cell wall stabilization, isolated from the resurrection plant Xerophyta viscosa, under stress-inducible XvPSap1 promoter via Agrobacterium-mediated transformation. Detection of the transgene by PCR, Southern blot, and quantitative real-time PCR (qRT-PCR) analyses revealed the integration of XvSap1 in the three independent events. Phenotypic evaluation of shoot length, number of leaves, and yield revealed that the transgenic plants grew better than the wild-type plants under drought stress. Assessment of biochemical indices during drought stress showed higher levels of chlorophyll, free proline, and relative water content and decreased lipid peroxidation in transgenic plants than in wild types. Our findings demonstrate that XvSap1 enhances drought tolerance in transgenic sweet potato without causing deleterious phenotypic and yield changes. The transgenic drought-tolerant sweet potato lines provide a valuable resource as a drought-tolerant crop on arid lands of the world.

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Section: Discussionmentioning

confidence: 99%

Induced Expression of Xerophyta viscosa XvSap1 Gene Enhances Drought Tolerance in Transgenic Sweet Potato

2019

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“…The H 2 O 2 and O 2 ·− contents were determined using an assay kit (COMINBIO; ) and using UV-1800 spectrophotometer (SHIMADZU; Gifu, Japan), following the manufacturer’s instructions [ 53 ]. The absorbance was recorded at 415 and 530 nm, respectively.…”

Section: Methodsmentioning

confidence: 99%

24-Epibrassinolide Ameliorates Endogenous Hormone Levels to Enhance Low-Temperature Stress Tolerance in Cucumber Seedlings

Anwar

Bai

et al. 2018

IJMS

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Phytohormone biosynthesis and accumulation are essential for plant growth and development and stress responses. Here, we investigated the effects of 24-epibrassinolide (EBR) on physiological and biochemical mechanisms in cucumber leaves under low-temperature stress. The cucumber seedlings were exposed to treatments as follows: NT (normal temperature, 26 °C/18 °C day/night), and three low-temperature (12 °C/8 °C day/night) treatments: CK (low-temperature stress); EBR (low-temperature and 0.1 μM EBR); and BZR (low-temperature and 4 μM BZR, a specific EBR biosynthesis inhibitor). The results indicated that low-temperature stress proportionately decreased cucumber seedling growth and the strong seedling index, chlorophyll (Chl) content, photosynthetic capacity, and antioxidant enzyme activities, while increasing reactive oxygen species (ROS) and malondialdehyde (MDA) contents, hormone levels, and EBR biosynthesis gene expression level. However, EBR treatments significantly enhanced cucumber seedling growth and the strong seedling index, chlorophyll content, photosynthetic capacity, activities of antioxidant enzymes, the cell membrane stability, and endogenous hormones, and upregulated EBR biosynthesis gene expression level, while decreasing ROS and the MDA content. Based on these results, it can be concluded that exogenous EBR regulates endogenous hormones by activating at the transcript level EBR biosynthetic genes, which increases antioxidant enzyme capacity levels and reduces the overproduction of ROS and MDA, protecting chlorophyll and photosynthetic machinery, thus improving cucumber seedling growth.

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“…The concentration of H 2 O 2 and O 2 •− were determined by using an assay kit (COMINBIO) with a UV-1800 spectrophotometer, following the manufacturer’s instructions [ 60 ].…”

Section: Methodsmentioning

confidence: 99%

5-Aminolevulinic Acid Improves Nutrient Uptake and Endogenous Hormone Accumulation, Enhancing Low-Temperature Stress Tolerance in Cucumbers

Anwar

Yan

Liu

et al. 2018

IJMS

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5-aminolevulinic acid (ALA) increases plant tolerance to low-temperature stress, but the physiological and biochemical mechanisms that underlie its effects are not fully understood. To investigate them, cucumber seedlings were treated with different ALA concentrations (0, 15, 30 and 45 mg/L ALA) and subjected to low temperatures (12/8 °C day/night temperature). The another group (RT; regular temperature) was exposed to normal temperature (28/18 °C day/night temperature). Low-temperature stress decreased plant height, root length, leaf area, dry mass accumulation and the strong seedling index (SSI), chlorophyll contents, photosynthesis, leaf and root nutrient contents, antioxidant enzymatic activities, and hormone accumulation. Exogenous ALA application significantly alleviated the inhibition of seedling growth and increased plant height, root length, hypocotyl diameter, leaf area, and dry mass accumulation under low-temperature stress. Moreover, ALA increased chlorophyll content (Chl a, Chl b, Chl a+b, and Carotenoids) and photosynthetic capacity, net photosynthetic rate (Pn), stomatal conductance (Gs), intercellular CO2 concentration (Ci), and transpiration rate (Tr), as well as the activities of superoxide dismutase (SOD), peroxidase (POD, catalase (CAT), ascorbate peroxidase (APX), and glutathione reductase (GR) enzymes, while decreasing hydrogen peroxide (H2O2), superoxide (O2•−), and malondialdehyde (MDA) contents under low-temperature stress. In addition, nutrient contents (N, P, K, Mg, Ca, Cu, Fe, Mn, and Zn) and endogenous hormones (JA, IAA, BR, iPA, and ZR) were enhanced in roots and leaves, and GA4 and ABA were decreased. Our results suggest the up-regulation of antioxidant enzyme activities, nutrient contents, and hormone accumulation with the application of ALA increases tolerance to low-temperature stress, leading to improved cucumber seedling performance.

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Heterotrimeric G-Protein γ Subunit CsGG3.2 Positively Regulates the Expression of CBF Genes and Chilling Tolerance in Cucumber

Cited by 35 publications

References 48 publications

Induced Expression of Xerophyta viscosa XvSap1 Gene Enhances Drought Tolerance in Transgenic Sweet Potato

Induced Expression of Xerophyta viscosa XvSap1 Gene Enhances Drought Tolerance in Transgenic Sweet Potato

24-Epibrassinolide Ameliorates Endogenous Hormone Levels to Enhance Low-Temperature Stress Tolerance in Cucumber Seedlings

5-Aminolevulinic Acid Improves Nutrient Uptake and Endogenous Hormone Accumulation, Enhancing Low-Temperature Stress Tolerance in Cucumbers

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