Heterotrimeric G protein signaling in the<i>Arabidopsis</i>unfolded protein response

Wang, Shiyu; Narendra, Savitha; Fedoroff, Nina V.

doi:10.1073/pnas.0611735104

Cited by 95 publications

(111 citation statements)

References 58 publications

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“…In both situations plant cells activate a developmental program that is equivalent to the yeast and mammalian UPR, and can be experimentally induced by the treatment with ER-stress inducers such as tunicamycin, an inhibitor of N-glycosylation [42,43].…”

Section: Deleted: Alsomentioning

confidence: 99%

Manufacturing antibodies in the plant cell

Orzáez

Granell

Blázquez

2009

Biotechnology Journal

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International audiencePlants have long been considered advantageous platforms for large-scale production of antibodies due to their low cost, scalability, and the low chances of pathogen contamination. Much effort has therefore been devoted to efficiently produce mAbs (from nanobodies to secretory antibodies) in plant cells. Several technical difficulties have been encountered and are being coped with. Improvements in production levels have been achieved by manipulation of gene expression and, more efficiently, of cell targeting and protein folding and assembly. Differences in mAb glycosylation patterns between animal and plant cells are being successfully addressed by the elimination and introduction of the appropriate enzyme activities in plant cells. Another relevant battlefield is the dichotomy between production capacity and speed. Classically, stably transformed plant lines have been proposed for large scale mAb production, whereas the use of transient expression systems has always provided production speed at the cost of scalability. However, recent advances in transient expression techniques have brought impressive yield improvements, turning speed and scalability highly compatible assets. In the era of personalized medicines, the combination of yield and speed, and the advances in glyco-engineering have made the plant cell a serious contender in the field of recombinant antibody production

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Section: Deleted: Alsomentioning

confidence: 99%

Manufacturing antibodies in the plant cell

Orzáez

Granell

Blázquez

2009

Biotechnology Journal

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“…Loss of specific RNA expression in the mutants was confirmed by RT-PCR analysis (Supplementary information, Figure S1B and S1C). To examine whether both HRD3A and HRD3B are active components of the ERAD complex in Arabidopsis, hrd3a-1, and hrd3b-1 mutants were grown on medium containing 0.2 µg/ml TM, an ER stress inducer through inhibiting protein glycosylation [28]. Surprisingly, only the growth of hrd3a-1, but not hrd3b-1, was sensitive to TM (Supplementary information, Figure S2B).…”

Section: Hrd3a Is the Only Functional Homolog Of Yeast Hrd3 In Arabidmentioning

confidence: 99%

The endoplasmic reticulum-associated degradation is necessary for plant salt tolerance

et al. 2010

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Eukaryotic organisms have quality-control mechanisms that allow misfolded or unassembled proteins to be retained in the endoplasmic reticulum (ER) and subsequently degraded by ER-associated degradation (ERAD). The ERAD pathway is well studied in yeast and mammals; however, the biological functions of plant ERAD have not been reported. Through molecular and cellular biological approaches, we found that ERAD is necessary for plants to overcome salt stress. Upon salt treatment ubiquitinated proteins increased in plant cells, especially unfolded proteins that quickly accumulated in the ER and subsequently induced ER stress responses. Defect in HRD3A of the HRD1/ HRD3 complex of the ERAD pathway resulted in alteration of the unfolded protein response (UPR), increased plant sensitivity to salt, and retention of ERAD substrates in plant cells. Furthermore, we demonstrated that Ca 2+ release from the ER is involved in the elevation of UPR and reactive oxygen species (ROS) participates the ERAD-related plant salt response pathway.

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“…[5][6][7][8][9] This does not necessarily underestimate the importance of heterotrimeric G protein-mediated signal transduction in plants; knocking out of sole gene AGB1 causes various developmental defects such as lateral root formations and abnormal organ shapes in leaves and flowers. 10,11 Intriguingly, AGB1 is involved in unfolded protein response (UPR), 12,13 a pathway in so-called endoplasmic reticulum (ER) quality control to cope with ER stresses. 14,15 .…”

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confidence: 99%