2004
DOI: 10.1152/ajpheart.00677.2003
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Heterotrimeric G protein Giis involved in a signal transduction pathway for ATP release from erythrocytes

Abstract: Erythrocytes are reported to release ATP in response to mechanical deformation and decreased oxygen tension. Previously we proposed that receptor-mediated activation of the heterotrimeric G protein G(s) resulted in ATP release from erythrocytes. Here we investigate the hypothesis that activation of heterotrimeric G proteins of the G(i) subtype are also involved in a signal transduction pathway for ATP release from rabbit erythrocytes. Heterotrimeric G proteins G(alphai1), G(alphai2), and G(alphai3) but not G(a… Show more

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Cited by 84 publications
(142 citation statements)
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“…Thus, in this study we analyzed the time dependent balance of ATP efflux and ATPe hydrolysis to determine the factors governing the effective extracellular concentration of the nucleotide, focusing on a condition when ATPe levels are elevated. Specifically, we made use of the fact that over the past years a growing body of evidence indicated that in human erythrocytes an increase in cAMP elicited by various physiological stimuli can induce a regulated release of ATP (8,43,47,49,50). The kinetic properties of this ATP efflux has so far received little attention and therefore, before studying the main features of ATP efflux in human erythrocytes, we established the optimal conditions leading to a cAMP-dependent release of ATP.…”
Section: Discussionmentioning
confidence: 99%
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“…Thus, in this study we analyzed the time dependent balance of ATP efflux and ATPe hydrolysis to determine the factors governing the effective extracellular concentration of the nucleotide, focusing on a condition when ATPe levels are elevated. Specifically, we made use of the fact that over the past years a growing body of evidence indicated that in human erythrocytes an increase in cAMP elicited by various physiological stimuli can induce a regulated release of ATP (8,43,47,49,50). The kinetic properties of this ATP efflux has so far received little attention and therefore, before studying the main features of ATP efflux in human erythrocytes, we established the optimal conditions leading to a cAMP-dependent release of ATP.…”
Section: Discussionmentioning
confidence: 99%
“…First, fluorescence microscopy was used to measure hemolysis as described earlier (8). Briefly, 3 ϫ 10 6 erythrocytes were loaded with BCECF and the retention of the intracellular fluorophore was assessed before and after addition of pharmacological agents.…”
Section: Extracellular Hydrolysis Of Atp Adenosine Production and Ementioning
confidence: 99%
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“…A signal transduction pathway that relates both pharmacological and physiological stimuli to ATP release from erythrocytes has been described. Components of this pathway include the heterotrimeric G proteins G s (24) and G i (25,26), adenylyl cyclase (AC) (16), cyclic AMP-dependent protein kinase A (16), and the cystic fibrosis transmembrane conductance regulator (11,27).…”
mentioning
confidence: 99%
“…The TTX-sensitive Na ϩ flux was also found to be inhibited by pertussis toxin, which implies that the increase in Na ϩ flux is G protein-mediated (4). There is ample evidence that heterotrimeric G proteins of several types (e.g., G i , G o , G q ) are present in human red blood cells (8)(9)(10)(11)(12)(13)(14); in addition, these cells are also known to contain Ϸ2.7 M Al 3ϩ ͞l.rbc (15), which may be necessary for adenylyl cyclase activation (16). It was further shown that, under conditions where the Na ϩ flux was increased in the presence of added F Ϫ and Ca 2ϩ , Ca 2ϩ uptake (measured with 45 Ca 2ϩ ) was also increased and could be inhibited with TTX (17).…”
mentioning
confidence: 99%