Heterotopic Osteogenesis by Murine Demineralized Incisors at Lesions Sites Induced by Concanavalin A in Mice

Włodarski, Paweł; Galus, Ryszard; Włodarski, K; Brodzikowska, Aniela

doi:10.1080/03008200802690661

Cited by 4 publications

(6 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Murine incisors hydrolyzed for 4–5 hr in chilled 0.6 N HCl were fully demineralized and exhibited good osteoinductive potency. We previously showed that 22 hr demineralization slightly diminishes their osteoinductive power, most likely by affecting their organic phase [26]. …”

Section: Discussionmentioning

confidence: 99%

“…Perhaps some traces of mineral remained following the procedure, but did not affect bone induction. The prolonged hydrolysis of murine incisors for 22 hr slightly reduces the yield of bone induction [26]. …”

Section: Methodsmentioning

confidence: 99%

“…Isolated human osteoblast proliferation may be either activated or inhibited by inflammatory cytokines, depending on their concentration and duration of exposure [24], whereas adjuvant-evoked granuloma cells activate endosteal osteogenesis [25]. Lesions induced by T-cell mitogen Concanavalin A have increased the yield of demineralized tooth-induced bone [26]. …”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effects of Time of Initial Exposure to MSV Sarcoma on Bone Induction by Dentine Matrix Implants and on Orthotopic Femora

Włodarski

Galus

et al. 2010

IJMS

Self Cite

View full text Add to dashboard Cite

HCl-demineralized murine lower incisors were implanted intramuscularly into syngeneic BALB/c mice to induce heterotopic osteogenesis. Implants were exposed at the early, preosteogenic stage (4), or at the later, osteogenic stage (12) to the Moloney sarcoma virus (MSV), which within 3–4 days results in a sarcoma. The yield of bone induction was determined by weight of dry bone mass following NaOH hydrolysis of soft tissues. To verify the effect of this sarcoma on orthotopic local femoral bone, the dry mass of the tumor-exposed femora was measured and compared with the weight of MSV-unexposed contralateral controls. MSV-sarcoma or cells involved with their spontaneous rejection have a stimulatory effect on the periosteal membrane of the tumor-adjacent femoral bones, increasing their dry mass on average by 18%. No stimulatory effect on heterotopic bone induction was observed when the MSV sarcoma grew during the early, preosteogenic stage (4 onward), but when the tooth matrix had been exposed to such tumor at the already bone-forming stage, (12 onward), the yield of bone induction was enhanced. Thus, it is postulated that lesions induced by MSV during the early, preosteogenic stage inhibit recruitment of osteoprogenitor cells or degrade Bone Morphogenetic Proteins (BMPs) released by matrix resorbing inflammatory cells, whereas when acting on already existing bone they have a stimulatory effect.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of Time of Initial Exposure to MSV Sarcoma on Bone Induction by Dentine Matrix Implants and on Orthotopic Femora

Włodarski

Galus

et al. 2010

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…Heterotopic bone formation was induced in 51 healthy female mice by intramuscular implantation of demineralized for 20 hrs in 0.6 N HCl at +4°C murine incisors according to the procedure described previously [19]. Under anesthesia, the implants were aseptically inserted into an intramuscular pocket executed in the thigh muscle by sharp scissors, following shaving of the surgery area.…”

Section: Methodsmentioning

confidence: 99%

The adipocyte component of bone marrow in heterotopic bone induced by demineralized incisor grafts

Włodarski¹,

Galus²,

Brodzikowska³

et al. 2012

Folia Histochem Cytobiol.

Self Cite

View full text Add to dashboard Cite

Abstract:The relative proportion of adipocytes to hematopoietic elements in the marrow of heterotopically induced bone evaluated 4-42 weeks post implantation of demineralized murine incisors was estimated by histological analysis of hematoxylin-eosin stained tissue sections. Using computerized image analysis of microphotographs, the proportion of nuclear cells vs. adipocytes was ascertained. The percentage of adipocytes in marrow increases over time. Such an effect, the replacement of myelopoietic marrow by adipogenic (yellow) marrow and the resorption of induced bone, is observed in human osteoporosis. A decline in the non-adipogenic cell compartments of bone marrow accompanying induced bone begins in the fourth week of induction, gradually progresses until the 26 th week, and does not change after that. The luminosity, a parameter used in image analysis and proportional to the number of nuclear cells, was 124 ± 3 in hematopoietic femoral bone marrow, and that of bone marrow of the induced bone was of a similar value (117 ± 8) in the fourth week. An evident decline in luminosity of bone marrow filling the foci of heterotopic bone was observed in samples taken at nine weeks (82 ± 20). This process progressed until the 26 th week, reaching a luminosity of 70 ± 21. At the 42 nd week, the luminosity remained at the same level (71 ± 27). This indicates that the replacement of hematopoietic bone marrow of heterotopically induced bone by unilocular adipocytes begins relatively early (the fourth week) and is persistent.

show abstract

“…Glycoconjugates that react with endogenous lectins can induce chondrogenesis and osteogenesis. For instance, the local administration of ConA increased demineralization [11]. The osteogenic cell population in mouse bone marrow cultures was found to bind WGA and synthesize bone-specific proteins such as alkaline phosphatase, type I collagen and osteocalcin, and to form mineralized nodules [12].…”

Section: Introductionmentioning

confidence: 99%

Lectins influence chondrogenesis and osteogenesis in limb bud mesenchymal cells

2011

View full text Add to dashboard Cite

The role of cell surface glycoproteins in cell behavior can be characterized by their interactions with plant lectins. This study was designed to identify the effects of lectins on chondrogenesis and osteogenesis in limb bud mesenchymal cells in vitro. Limb bud mesenchymal cells from mouse embryos were cultured in high-density micromass culture. Wheat germ agglutinin (WGA), concanavalin A (ConA), peanut agglutinin (PNA), Dolichos biflorus agglutinin (DBA) and Ricinus communis agglutinin (RCA) were added separately to the culture media. Cells were cultured for 5 or 9 days, and cell viability was assayed by neutral red on day 5. The micromasses were stained with alcian blue, alizarin red S and Von Kossa stains, and alkaline phosphatase assays were also done. Dolichos biflorus agglutinin induced an increase in chondrogenesis, calcium precipitation and proteoglycan production. ConA and PNA did not affect chondrocyte differentiation but induced chondrocytes to produce more proteoglycan. Wheat germ agglutinin reduced chondrification and ossification but induced mesenchymal cells to store lipid droplets. Ricinus communis agglutinin 1 was toxic and significantly reduced cell survival. In conclusion, DBA was the most effective inducer of ossification and chondrification. Wheat germ agglutinin induced adipogenesis instead. These assays showed that lectins play important roles in limb bud development.

show abstract

Heterotopic Osteogenesis by Murine Demineralized Incisors at Lesions Sites Induced by Concanavalin A in Mice

Cited by 4 publications

References 31 publications

Effects of Time of Initial Exposure to MSV Sarcoma on Bone Induction by Dentine Matrix Implants and on Orthotopic Femora

Effects of Time of Initial Exposure to MSV Sarcoma on Bone Induction by Dentine Matrix Implants and on Orthotopic Femora

The adipocyte component of bone marrow in heterotopic bone induced by demineralized incisor grafts

Lectins influence chondrogenesis and osteogenesis in limb bud mesenchymal cells

Contact Info

Product

Resources

About