Heterotopic mineral deposits in intact rat Achilles tendons are characterized by a unique fiber-like structure

Pierantoni, M.; Hammerman, Malin; Barreto, Isabella Silva; Andersson, Linnea; Novák, Vladimír; Isakson, Hanna; Eliasson, Pernilla

doi:10.1016/j.yjsbx.2023.100087

Cited by 6 publications

(12 citation statements)

References 61 publications

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“…By using high‐resolution phase‐contrast synchrotron X‐ray tomography this study provides new information on the spatiotemporal formation, structure, and growth of HO deposits in healing rat Achilles tendons. We identified HO deposits with fiber‐like internal structure (visible both in the tomograms, Figure 3) close to the calcaneal bone in all the intact tendons similarly to what we recently reported 35 . The fiber‐like structure allowed us to distinguish the pre‐existing deposits from HO formed during the healing process 35 .…”

Section: Discussionsupporting

confidence: 81%

“…Adipose tissue in healing tendons has previously been described in other studies, but not encapsulated in HO deposits 19,45 . The spheric deposits that we observed are similar to the adipose tissue which can also be observed around intact tendons 33 and within some of the HO deposits protruding out from intact tendons 35 . The presence of adiopose‐like tissue inside some HO deposits could explain previous histological observation of unstained regions (and consequently neither mineral nor cartilage) in the center of some deposits at weeks 12 and 16, 8 and the presence of the empty rings observed in histological images in this study at weeks 12 and 20.…”

Section: Discussionsupporting

confidence: 81%

“…We identified HO deposits with fiber-like internal structure (visible both in the tomograms, Figure 3) close to the calcaneal bone in all the intact tendons similarly to what we recently reported. 35 The fiber-like structure allowed us to distinguish the pre-existing deposits from HO formed during the healing process. 35 The pre-existing HO deposits were also clearly visible at 1 week, and the size (in some cases up to 1.2 mm 3 ) was comparable to what we observed in intact tendons 35 .…”

Section: Discussionmentioning

confidence: 99%

“…35 The fiber-like structure allowed us to distinguish the pre-existing deposits from HO formed during the healing process. 35 The pre-existing HO deposits were also clearly visible at 1 week, and the size (in some cases up to 1.2 mm 3 ) was comparable to what we observed in intact tendons 35 . However, new mineral deposition at some of the edges could be already observed (Figure S3).…”

Section: Discussionmentioning

confidence: 99%

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Spatiotemporal and microstructural characterization of heterotopic ossification in healing rat Achilles tendons

et al. 2023

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Achilles tendon rupture is a common debilitating medical condition. The healing process is slow and can be affected by heterotopic ossification (HO), which occurs when pathologic bone-like tissue is deposited instead of the soft collagenous tendon tissue. Little is known about the temporal and spatial progression of HO during Achilles tendon healing. In this study we characterize HO deposition, microstructure, and location at different stages of healing in a rat model. We use phase contrast-enhanced synchrotron microtomography, a state-of-the-art technique that allows 3D imaging at high-resolution of soft biological tissues without invasive or time-consuming sample preparation. The results increase our understanding of HO deposition, from the early inflammatory phase of tendon healing, by showing that the deposition is initiated as early as one week after injury in the distal stump and mostly growing on preinjury HO deposits. Later, more deposits form first in the stumps and then all over the tendon callus, merging into large, calcified structures, which occupy up to 10% of the tendon volume. The HOs were characterized by a looser connective trabecular-like structure and a proteoglycanrich matrix containing chondrocyte-like cells with lacunae. The study shows the potential of 3D imaging at high-resolution by phase-contrast tomography to better understand ossification in healing tendons.

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Section: Discussionsupporting

confidence: 81%

Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Spatiotemporal and microstructural characterization of heterotopic ossification in healing rat Achilles tendons

et al. 2023

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“…Focusing on the T/L tissue instead, due to the high resolution and contrast required, microCT studies were performed mostly in static conditions by dehydrating samples, using contrast agents, or eventually performing phase-contrast synchrotron x-ray images [37][38][39][40][41][42]. To the authors' knowledge, only one study has been carried out so far using DVC to study strain distributions in the rat enthesis [43].…”

Section: Introductionmentioning

confidence: 99%

Full-field strain distribution in hierarchical electrospun nanofibrous Poly-L(lactic) acid and Collagen based scaffolds for tendon and ligament tissue regeneration: a multiscale study

Sensini

Stamati

Marchiori

et al. 2023

Preprint

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Regeneration of injured tendons and ligaments (T/L) is a worldwide need. In this study electrospun hierarchical scaffolds made of a poly (L-lactic) acid and collagen blend were developed reproducing all the multiscale levels of aggregation of these tissues. Scanning electron microscopy, microCT and tensile mechanical tests were carried out, including a multiscale digital volume correlation analysis to measure the full-field strain distribution of electrospun structures. The principal tensile and compressive strains detected the pattern of strains caused by the nanofibers rearrangement, while the deviatoric strains revealed the related internal sliding of nanofibers and bundles. The results of this study confirmed the biomimicry of such electrospun hierarchical scaffolds, paving the way to further tissue engineering and clinical applications.

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In Situ Loading and Time‐Resolved Synchrotron‐Based Phase Contrast Tomography for the Mechanical Investigation of Connective Knee Tissues: A Proof‐of‐Concept Study

Dejea,

Pierantoni,

Orozco

et al. 2024

Advanced Science

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Articular cartilage and meniscus transfer and distribute mechanical loads in the knee joint. Degeneration of these connective tissues occurs during the progression of knee osteoarthritis, which affects their composition, microstructure, and mechanical properties. A deeper understanding of disease progression can be obtained by studying them simultaneously. Time‐resolved synchrotron‐based X‐ray phase‐contrast tomography (SR‐PhC‐µCT) allows to capture the tissue dynamics. This proof‐of‐concept study presents a rheometer setup for simultaneous in situ unconfined compression and SR‐PhC‐µCT of connective knee tissues. The microstructural response of bovine cartilage (n = 16) and meniscus (n = 4) samples under axial continuously increased strain, or two steps of 15% strain (stress–relaxation) is studied. The chondrocyte distribution in cartilage and the collagen fiber orientation in the meniscus are assessed. Variations in chondrocyte density reveal an increase in the top 40% of the sample during loading, compared to the lower half. Meniscus collagen fibers reorient perpendicular to the loading direction during compression and partially redisperse during relaxation. Radiation damage, image repeatability, and image quality assessments show little to no effects on the results. In conclusion, this approach is highly promising for future studies of human knee tissues to understand their microstructure, mechanical response, and progression in degenerative diseases.

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Heterotopic mineral deposits in intact rat Achilles tendons are characterized by a unique fiber-like structure

Cited by 6 publications

References 61 publications

Spatiotemporal and microstructural characterization of heterotopic ossification in healing rat Achilles tendons

Spatiotemporal and microstructural characterization of heterotopic ossification in healing rat Achilles tendons

Full-field strain distribution in hierarchical electrospun nanofibrous Poly-L(lactic) acid and Collagen based scaffolds for tendon and ligament tissue regeneration: a multiscale study

In Situ Loading and Time‐Resolved Synchrotron‐Based Phase Contrast Tomography for the Mechanical Investigation of Connective Knee Tissues: A Proof‐of‐Concept Study

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