Heterosis and reselection for pyrethroid resistance trait maintenance in the lady beetle Eriopis connexa (Germar)

Abstract

Exposure of Eriopis connexa (Germar) to pyrethroid residues in agroecosystems has resulted in selection for resistance (R). Pyrethroid resistance allows E. connexa to survive lambda‐cyhalothrin applications. Following a field release of E. connexa, development of resistance in an incipient population may depend on three major factors such as the maintenance of: (i) selection pressure, (ii) frequency of mating with susceptible phenotypes (S) and (iii) differential reproductive performance due to the fitness costs associated with resistance. To investigate the potential effects of these three factors on the development of pyrethroid resistance by progeny of field released E. connexa, our experiments included panmictic mating between R and S phenotypes, followed by descendant rearing with and without insecticide selection pressure, reselection and determination of resistance levels. In addition, we measured the reproductive performance of the parental R and S phenotypes and their descendants to assess the cost of resistance after crossing and reselection. Survival of R × S descendants exposed to lambda‐cyhalothrin was reduced across successive generations in the absence of selection pressure, but still enhanced after four generations indicating the persistent presence of resistant phenotypes in the population. Under selection pressure with exposure to lambda‐cyhalothrin applied at label rates, descendant survival was >50%. Fecundity and survival were higher in the first‐generation of crossed R × S females, but higher fecundity was not sustained after reselection. Adults of the R population exhibited a fitness cost, reduced longevity, when compared to S phenotypes and R × S crossed populations. Therefore, resistance maintenance in E. connexa after release will depend on selection pressures imposed by insecticide exposure. In the absence of selection pressure, the phenotype for resistance was reduced, but not completely lost. Further, resistant phenotypes can be reselected following insecticide exposure and this can explain, in part, the high frequency of field‐evolved resistance to lambda‐cyhalothrin in E. connexa.