Heterosis and combining ability in rice as influenced by introgressions from wild species Oryza rufipogon including qyld2.1 sub-QTL into the restorer line KMR3

Thalapati, Sudhakar; Guttikonda, Haritha; Nannapaneni, Naga Deepthi; Adari, Prasad Babu; Reddy, C. Suresh; Swamy, B. P. Mallikarjuna; Batchu, Anil K.; Basava, Ramana Kumari; Viraktamath, B. C.; Neelamraju, Sarla

doi:10.1007/s10681-014-1222-1

Cited by 18 publications

(14 citation statements)

References 34 publications

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“…Trials which are based on various mating designs and which are accompanied by statistical analysis not only differentiates between the effect of genetical and environmental factors but also partitions genetic influences into additive and non-additive components [32,34]. Such statistical analyses are possible only when an in-depth scrutiny of the relationship between combining ability of parental lines and per se heterosis of the novel hybrids is undertaken [35]. As opined by [36], combining ability is an estimate of the genotypic values of lines and testers in relation with their offsprings performance using a definite mating design, which is generally assessed through progeny testing.…”

Section: Discussionmentioning

confidence: 99%

Assessment of heterotic potential and combining ability of immortal restorer lines derived from an elite rice hybrid, KRH-2, for the development of superior rice hybrids

Kulkarni

Fiyaz

et al. 2020

Preprint

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Present investigation was carried out to assess the heterotic potential and combining ability of immortal restorer lines [consisting of two recombinant inbred lines (RILs) and two doubled haploid lines (DHLs)] developed from an elite rice hybrid, KRH-2 by crossing them with three popular WA-CMS lines, IR58025A, CRMS32A and APMS6A through line × tester analysis. The doubled haploid line 1 (DHL-1) was observed to be a good general combiner for total grain yield per plant (YLD) and other yield component traits and among the CMS lines, IR58025A was observed to be the best combiner as it showed positive significant values for the traits viz., total grain yield per plant, panicle length and spikelet fertility. Higher preponderance of the variance associated with specific combining ability (SCA) as compared to general combining ability (GCA) variance was observed for most of the traits indicated the predominant role of non-additive gene action in the expression of the traits. Out of twelve novel crosses between the immortal restorer lines derived from KRH-2 and the WA-CMS lines, 66.66% (eight crosses) showed significant and desirable SCA effects for the traits viz., total grain yield per plant, days to fifty percent flowering, plant height, flag leaf length, flag leaf width, number of filled grains per panicle and spikelet fertility. Two crosses IR58025A/RIL-24 and CRMS32A/RIL-24 were observed to be the most promising cross combinations showing standard heterosis of >50% for YLD trait (as compared with KRH-2) with higher prevalence of GCA and SCA, respectively. Heterotic yield advantage of IR58025A/RIL-24 and CRMS32A/RIL-24 was 77.05% and 54.74%, respectively over KRH-2 and these can be utilized for developing commercial hybrids. The present study also indicates the potentiality of RILs in providing useful parental lines for developing heterotic hybrids which are hard to get from outside sources in the new intellectual property regime.

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Section: Discussionmentioning

confidence: 99%

Assessment of heterotic potential and combining ability of immortal restorer lines derived from an elite rice hybrid, KRH-2, for the development of superior rice hybrids

Kulkarni

Fiyaz

et al. 2020

Preprint

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“…Such a comparative analysis of hybrids has not been made before. Our previous work showed that the hybrid IR79156A/IL50-13 gave significantly high yield 43 g/plant and showed highest specific combining ability (SCA) for yield (6.04) among 36 hybrids and high standard heterosis over KRH2 (61.79%) and DRRH2 (50.23%) indicating predominance of non-additive gene action for grain yield heterosis in test hybrid 9,10 . Several studies showed that non-additive gene action is important for yield and yield related traits in rice hybrids [31][32][33] .…”

Section: Discussionmentioning

confidence: 99%

“…The highest yielding top 11 of 67 hybrids which had introgressions from qyld2.1 showed 40.0-48.06 g yield per plant compared with respective control hybrids with 30.2-35.5.9 g 9 . An elite hybrid IR79156A/IL50-13 showed significantly high combining ability and standard heterosis for yield over popular hybrid DRRH2 9,10 . The test hybrid IR79156A/ IL50-13 (with O. rufipogon introgression) showed yield advantage of 32% over the control hybrid IR79156A/ KMR3 (without O. rufipogon introgression) during kharif season 2012 at IIRR (Indian Institute of Rice Research) farm, Rajendranagar, Hyderabad.…”

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confidence: 96%

Genome-wide transcriptome profile of rice hybrids with and without Oryza rufipogon introgression reveals candidate genes for yield

Guttikonda

Thummala

Agarwal

et al. 2020

Sci Rep

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In this study, we compared genome-wide transcriptome profile of two rice hybrids, one with (test hybrid IR79156A/IL50-13) and the other without (control hybrid IR79156A/KMR3) O. rufipogon introgressions to identify candidate genes related to grain yield in the test hybrid. IL50-13 (Chinsurah Nona2 IET21943) the male parent (restorer) used in the test hybrid, is an elite BC 4 f 8 introgression line of KMR3 with O. rufipogon introgressions. We identified 2798 differentially expressed genes (DEGs) in flag leaf and 3706 DEGs in panicle. Overall, 78 DEGs were within the major yield QTL qyld2.1 and 25 within minor QTL qyld8.2. The DEGs were significantly (p < 0.05) enriched in starch synthesis, phenyl propanoid pathway, ubiquitin degradation and phytohormone related pathways in test hybrid compared to control hybrid. Sequence analysis of 136 DEGs from KMR3 and IL50-13 revealed 19 DEGs with SNP/InDel variations. Of the 19 DEGs only 6 showed both SNP and InDel variations in exon regions. Of these, two DEGs within qyld2.1, Phenylalanine ammonia-lyase (PAL) (Os02t0626400-01, OsPAL2) showed 184 SNPs and 11 InDel variations and Similar to phenylalanine ammonia-lyase (Os02t0627100-01, OsPAL4) showed 205 SNPs and 13 InDel variations. Both PAL genes within qyld2.1 and derived from O. rufipogon are high priority candidate genes for increasing grain yield in rice. Rice (Oryza sativa) is an important food crop providing 20% of daily calories to more than 50 percent of global population. Nearly 90% of rice is produced and consumed in Asia. The continuous increase in human population, especially in Asia, poses a major challenge to food security. Therefore, enhancing grain yield is the primary thrust area of plant breeders. Hybrid rice can help increase productivity by 10-20% more than conventional varieties 1. Currently, the highest-yielding rice hybrids are developed from inter-subspecific crosses between indica and japonica 2-5. Wild species have been used to breed parental lines for yield improvement in derived rice hybrids 6-10. Flag leaf is the most essential functional organ to produce a large proportion of photo-assimilates that are stored in grains 11,12. It is estimated that it contributes around 32.3% of total carbohydrates during grain filling 13. The panicle morphology also directly affects the number and size of seeds and also determines grain yield in rice 14-16. Flag leaf and panicles have been used previously at different developmental stages to unravel gene expression in pollen development and genetic networks that control panicle branching and architecture 17,18. The spatial and temporal expression profiles of genes during 19 vegetative and reproductive stages of organ development were analysed to identify stage-preferential/stage-specific genes in IR64 variety and anther-specific genes in Pusa Basmati1 variety 19,20. A whole-genome oligonucleotide microarray of super hybrid LYP9 (Liangyoupeijiu) and its parents 9311 and PA64s in 7 different tissues showed that differentially expressed genes for energy metabolism i...

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“…In India, out of a total 44.15 million hectares under rice cultivation, hybrid rice occupies less than three million hectares [4]. Limited adoption of hybrid rice in India could be primarily attributed to the lower yield advantage of these hybrids over the conventional varieties, coupled with lack of good grain quality features in rice hybrids [5,56]. Therefore, in order to popularize the hybrids, there is an imminent requirement that the released novel hybrids have at least 20-30% yield advantage in comparison with the existing varieties along with the appropriate supporting policies for their better adoption [5,57,58].…”

Section: Discussionmentioning

confidence: 99%

“…physical position, 31.24 Mb, was earlier reported by [74] to possess a major QTL governing yield (GY) trait. As the two traits, PL and GY are positively correlated to each other [56,79], it is possible that a single candidate gene could be controlling both the traits. A second QTL hotspot region anked within the SSR markers, RM7023 (6.98 Mb) and RM586 (1.47 Mb) on chr 6 was observed to govern three traits namely YLD (qYLD6-1), FLL (qFLL6-1) and PL (qPL6-1)…”

Section: Qtl Hotspotsmentioning

confidence: 99%

QTL Mapping of Novel Genomic Regions for Yield Related Traits in Doubled Haploid (DH) Population Derived from the Popular Rice Hybrid KRH-2

Kulkarni

Ulaganathan

et al. 2020

Preprint

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Background: Rice, being the principal food crop and major nutritional source for more than half of the global population, is also an important source of livelihood in many South and South-East Asian countries. Amidst diminishing natural resources and many biotic-abiotic stresses, increasing the yield of rice varieties remains a challenging task. Identification of novel and yield augmenting alleles from stable rice hybrids is crucial to facilitate their marker-assisted transfer into various genetic backgrounds. Results: Quantitative trait loci (QTL) mapping using a population of 125 doubled haploid (DH) lines developed from the cross IR58025A/KMR3R and 126 polymorphic SSR; EST-derived SSR markers led to the identification of 12 each of major-minor effect QTLs for yield related traits. Major effect QTLs were detected for traits namely days to fifty percent flowering, test (1,000) grain weight, plant height, panicle weight, panicle length, flag leaf width, flag leaf length, biomass and total grain yield/plant explaining the phenotypic variability in the range of 29.95%-56.75%. QTL hotspots were detected on chromosome 3 for the traits, panicle length and total grain yield/plant and on chromosome 6 for the traits, panicle length, flag leaf length and total grain yield/plant. Though many of these QTLs were noted to co-localize with the QTL regions reported in earlier studies, five novel and major effect QTLs for panicle length, biomass, flag leaf width, panicle weight, plant height and three novel minor effect QTLs for panicle weight and fertile grains per panicle, were identified in this study. Conclusions: Through this study, both major-minor effect novel QTLs for crucial yield related traits, viz., fertile grains per panicle, panicle length, panicle weight were identified. Further, the QTL hotspots identified on two different chromosomes for flag leaf length, panicle length and total grain yield/plant shall not only help in understanding the underlying genetic mechanisms of yield regulation but also would provide an insight into the genetic synchrony among the various yield related traits in contributing for yield heterosis. The identified QTL hotspots after their validation can be deployed in breeding programs targeted towards improvement of yield heterosis.

show abstract

Heterosis and combining ability in rice as influenced by introgressions from wild species Oryza rufipogon including qyld2.1 sub-QTL into the restorer line KMR3

Cited by 18 publications

References 34 publications

Assessment of heterotic potential and combining ability of immortal restorer lines derived from an elite rice hybrid, KRH-2, for the development of superior rice hybrids

Assessment of heterotic potential and combining ability of immortal restorer lines derived from an elite rice hybrid, KRH-2, for the development of superior rice hybrids

Genome-wide transcriptome profile of rice hybrids with and without Oryza rufipogon introgression reveals candidate genes for yield

QTL Mapping of Novel Genomic Regions for Yield Related Traits in Doubled Haploid (DH) Population Derived from the Popular Rice Hybrid KRH-2

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