Heteromorphic Self-Incompatibility In Primula: Twenty-First Century Tools Promise To Unravel A Classic Nineteenth Century Model System

McCubbin, Andrew G.

doi:10.1007/978-3-540-68486-2_14

Cited by 25 publications

(34 citation statements)

References 53 publications

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“…This distribution pattern indicated that stamen length did not differ between thrums and pins, unlike style length. In most heterostylous species of Primula, Fagopyrum, and Turnera, morphological polymorphism is observed not only in style length but also in stamen length (McCubbin, 2008). However, stamen structure is monomorphic in Linum (Barrett, 1992;Darwin, 1877), and our result supports this finding.…”

Section: Definition Of Thrum and Pin Morphs And Segregation Of Heterosupporting

confidence: 84%

“…Twenty-six and 25 AB14 F 1 population plants were scored as thrum and pin, respectively, and the thrum to pin ratio was 1:1. This segregation indicated that the floral morph of heterostyly in Linum was also controlled by a single diallelic locus, as in other typical heterostylous species of Primula and Fagopyrum (Barrett, 1992;McCubbin, 2008). The thrum is heterozygous (S/s) and the pin is homozygous recessive (s/s) in other species.…”

Section: Definition Of Thrum and Pin Morphs And Segregation Of Heteromentioning

confidence: 69%

“…Considering the tight linkage of the S locus genes (Barrett, 1992;McCubbin, 2008), it is unlikely but cannot be ruled out that recombination occurs between genes controlling SI and floral morph phenotypes, if two phenotypes are regulated by different genes at the S locus in Linum. Investigation of the SI phenotype may be required in future study in order to isolate the genes controlling SI.…”

Section: Definition Of Thrum and Pin Morphs And Segregation Of Heteromentioning

confidence: 99%

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Genetic Control of Floral Morph and Petal Pigmentation in <i>Linum grandiflorum</i> Desf., a Heterostylous Flax

et al. 2015

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Two types of floral morph, called thrum and pin, occur in heterostylous species. The thrum and pin flowers have shorter and longer styles, respectively. Heterostyly in Linum has been studied since Darwin's era. The floral morph, self-incompatibility, and related phenotypes have been well characterized using a natural population, but genetic analysis using a segregated population has not been reported, and the mode of inheritance of heterostyly in Linum remains to be investigated. We prepared a segregated population by crossing thrum and pin flowers of Linum grandiflorum Desf. and investigated style and stamen lengths. On the basis of the style to stamen length ratio, the population could be divided into thrum and pin clearly at a ratio of 1:1. Style length of pins was 1.6 times longer than that of thrums. To investigate the factor regulating the difference in style length, we further measured the style cell length of thrums and pins. The style cell length of pins was longer than that of thrums, whose rate was comparable to the rate of the style length ratio between thrum and pin flowers. These findings indicate that the floral morph of heterostyly in Linum is controlled by a single diallelic locus, and that a difference in the cell expansion rate caused thrum and pin morphs in the style, such as in a typical heterostylous species. PCR genotyping showed that TSS1, an S candidate gene reported previously, cosegregated completely with the thrum phenotype, indicating strong linkage between the S locus and TSS1. Furthermore, three flower colors (red, pink, and white) were observed at a 1:2:1 ratio, and no white thrum flowers or red pin flowers were found in this population. These flower color phenotypes could also be controlled by a diallelic locus, whose two alleles (R and r) would be incompletely dominant, and therefore, may be linked to the S locus.

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Section: Definition Of Thrum and Pin Morphs And Segregation Of Heterosupporting

confidence: 84%

Section: Definition Of Thrum and Pin Morphs And Segregation Of Heteromentioning

confidence: 69%

Section: Definition Of Thrum and Pin Morphs And Segregation Of Heteromentioning

confidence: 99%

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Genetic Control of Floral Morph and Petal Pigmentation in <i>Linum grandiflorum</i> Desf., a Heterostylous Flax

et al. 2015

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“…To identify the genes controlling distyly, we have initiated chromosome walking in Turnera (Labonne et al, 2009). Similar approaches are being used in Primula and Fagopyrum (Li et al, 2007;McCubbin, 2008;Yasui et al, 2008). Once a bacterial artificial chromosome contig of the S-locus region is assembled, the mutants generated will be invaluable in narrowing the specific region of the contig containing the S-locus genes of T. subulata.…”

Section: Discussionmentioning

confidence: 99%

Characterization of X-ray-generated floral mutants carrying deletions at the S-locus of distylous Turnera subulata

2010

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To investigate the genetic architecture of distyly in Turnera subulata and test the hypothesis that a supergene determines distyly, we used X-ray mutagenesis to generate floral mutants. Based upon the crossing design, all progeny were expected to be short-styled. Of 3982 progeny screened, 10 long-styled mutants, one long homostyle and one short homostyle were recovered. Assays for molecular markers tightly linked to the S-locus showed that the mutants were missing 1-3 markers indicating they are deletion mutants. We investigated the incompatibility phenotype of the mutants and found that both their styles and pollen behaved like those of the long-styled morph. There was a variation in the absolute length of styles, stamens and pollen size of the long-styled mutants. Furthermore, long-styled mutants possessing larger deletions tended to have their anthers and stigmas in closer proximity. We explored the inheritance of the S-locus mutations and found that only one of the deletion mutations was transmitted to progeny where we recovered seven such progeny. Remarkably, our data are consistent with the supergene model (GPA/gpa) of Primula. The long homostyle mutant appears to have deletions involving both the G and P loci. The other mutants appear to have deletions of the entire S-locus. The mutants generated will serve as a valuable resource for the molecular dissection of the S-locus region, and in the identification of genes determining distyly.

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“…also differ in ancillary traits, such as pollen grain size and number, stigmatic papillae morphology, stigma shape and corolla size (Dulberger, 1992;Richards, 1997;McCubbin, 2008;Ferrero et al, 2011;Cohen et al, 2012). Of the fl oral traits involved in distyly, reciprocal herkogamy is the main morphological component shaping fl oral morphs (Ganders, 1979).…”

mentioning

confidence: 99%

Distylous traits in Cordia dodecandra and Cordia sebestena (Boraginaceae) from the Yucatan Peninsula

Canché-Collí

Canto

2014

Bot. Sci.

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Abstract:Distyly is a genetically-controlled polymorphism with two fl oral morphs exhibiting spatial, reciprocal separation between stigmas and anthers; one morph presents styles above anthers, and the other styles below anthers. Distyly is usually linked to a self-incompatibility system that prevents self-pollination and crosses between plants of the same morph, thereby ensuring symmetrical mating and equal morph ratios within populations. This study is the fi rst description of distylous traits in the tropical tree species Cordia dodecandra and C. sebestena from the Yucatan Peninsula. Principal components analysis of fl ower morphological traits identifi ed segregation into two groups with characteristically distylous morphology. Estimation of reciprocal herkogamy using indices showed almost perfect reciprocity in the distance between long and short sexual organs in the two fl ower morphs of both species. Reciprocity between organs was lower in C. dodecandra, suggesting less accuracy in pollen transference between morphs. Self-incompatibility was observed for C. dodecandra, but could not be confi rmed for C. sebestena. However, a 1:1 fl oral morph ratio within the sampled C. sebestena populations suggests the existence of a distylous, self-incompatibility system in this species. Morph distribution in C. dodecandra did not follow a typically distylous distribution, suggesting a disruption in population processes such as seed dispersion and seedling establishment in this species. The reported data confi rm the existence of the distyly syndrome in the two Cordia species. Key words: distyly, morph ratio, reciprocal herkogamy, self-incompatibility.Resumen: La distilia es la separación recíproca entre estigma y anteras, entre plantas de una población, pudiéndose distinguir individuos con fl ores de estilo largo y anteras cortas e individuos con fl ores de estilo corto y anteras largas. Involucrados en este polimorfi smo se encuentran rasgos morfológicos de las fl ores y una auto-incompatibilidad controlada por un sistema de dos alelos que resulta en una proporción de tipos fl orales uno a uno dentro de una población. En este trabajo se explora las características de distilia en dos especies cercanas de árboles tropicales que se distribuyen en la duna costera y en los remanentes de huertos mayas en Yucatán. El análisis de componentes principales mostró la segregación de dos grupos de individuos con diferente morfología fl oral en ambas especies. Los índices de reciprocidad señalaron que la hercogamia entre los órganos largos y cortos se ajusta de forma casi perfecta a lo esperado en un sistema distílico con autoincompatibilidad sexual en Cordia sebestena y C. dodecandra. Sin embargo, en esta última especie se observó una mayor variación en la precisión en la posición de los órganos recíprocos, pudiendo tener consecuencias en la exactitud con la que el polen es depositado sobre el estigma. Las pruebas de incompatibilidad realizadas únicamente en C. dodecandra mostraron que los individuos cuyas fl ores recibieron polen...

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Heteromorphic Self-Incompatibility In Primula: Twenty-First Century Tools Promise To Unravel A Classic Nineteenth Century Model System

Cited by 25 publications

References 53 publications

Genetic Control of Floral Morph and Petal Pigmentation in <i>Linum grandiflorum</i> Desf., a Heterostylous Flax

Genetic Control of Floral Morph and Petal Pigmentation in <i>Linum grandiflorum</i> Desf., a Heterostylous Flax

Characterization of X-ray-generated floral mutants carrying deletions at the S-locus of distylous Turnera subulata

Distylous traits in Cordia dodecandra and Cordia sebestena (Boraginaceae) from the Yucatan Peninsula

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