Heterologous Reassortment of Bunyaviruses in Aedes Triseriatus Mosquitoes and Transovarial and Oral Transmission of Newly Evolved Genotypes

Chandler, Laura J.; Beaty, Barry J.; Baldridge, Gerald D.; Bishop, David H. L.; Hewlett, Martinez J.

doi:10.1099/0022-1317-71-5-1045

Cited by 30 publications

(21 citation statements)

References 9 publications

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“…Similar interference to reassortment has been described previously when invertebrate hosts are orally infected with two ts mutants of LAC virus (Beaty et al, 1985) and in infections involving two strains of Rift Valley fever virus (Turell et al, 1990). Co-infection, or superinfection of mosquitoes with SSH virus 1 h after LAC virus infection, results in reassortant progeny (Chandler et al, 1990). Our data from cultured vertebrate cells agree with the view that the opportunity for reassortment may depend on the time of initiation of parent virus replication.…”

Section: Discussionmentioning

confidence: 71%

“…Previous studies have demonstrated reassortment between bunyaviruses (Gentsch et al, 1977(Gentsch et al, b, 1979(Gentsch et al, , 1980Rozhon et al, 1981;Pringle et al, 1984;Janssen et al, 1986;Chandler et al, 1990Chandler et al, , 1991Endres et al, 1991) using direct and inferred genotype determinations. The PCR method that we adopted to analyse a large number of reassortant viruses was based upon the exploitation of a few nucleotide differences between SSH and LAC viruses.…”

Section: Discussionmentioning

confidence: 99%

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Non-random reassortment between the tripartite RNA genomes of La Crosse and snowshoe hare viruses

Urquidi¹,

Bishop²

1992

Journal of General Virology

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The process of reassortment between the tripartite RNA genomes (segments designated L, M and S) of snowshoe hare and La Crosse bunyaviruses (Bunyaviridae) has been investigated by polymerase chain reaction analysis of > 250 progeny recovered at 72 h postinfection from dual wild-type virus infections involving high multiplicities (approximately 5) of each virus in a BHK-21 cell line. Statistical analysis of the data indicated that RNA segment reassortment was not random, and for these two viruses the data appeared to fit the hypothesis that there was a preference for homologous L-M and M-S associations among the progeny formed.

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Section: Discussionmentioning

confidence: 71%

Section: Discussionmentioning

confidence: 99%

Non-random reassortment between the tripartite RNA genomes of La Crosse and snowshoe hare viruses

Urquidi¹,

Bishop²

1992

Journal of General Virology

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“…In some of those experiments that dealt with vertical and venereal transmission, an enhancement of those transmission modes was observed after a noninfectious blood feeding (as compared to rates among nonblood-fed mosquitoes) (Chandler et al 1990). Our experimental design did not control for the possibility that prior blood feeding could alter virus infection/dissemination rates.…”

Section: Discussionmentioning

confidence: 99%

Effect of Sequential Exposure on Infection and Dissemination Rates for West Nile and St. Louis Encephalitis Viruses inCulex quinquefasciatus

Pesko

2009

Vector-Borne and Zoonotic Diseases

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West Nile virus has spread rapidly throughout the United States since its introduction in 1999, into some areas that are also endemic for St. Louis encephalitis virus (SLEV). These viruses are in the same antigenic complex within the genus Flavivirus, family Flaviviridae. Further, both viruses are transmitted primarily by Culex spp. mosquitoes and use birds as amplifying hosts. These viruses could contemporaneously coinfect individual vectors wherein changes in mosquito immune responses might occur. To explore this possibility, we evaluated the effect of sequential infection with both West Nile virus and SLEV on the infection and dissemination rates of these viruses in the vector mosquito, Culex quinquefasciatus. Prior exposure to either virus lowered susceptibility to infection with the second virus, and lower dissemination rates of the second virus, compared to controls. Exposure to one virus followed by a second virus resulted in similar infection rates for the first virus to those of controls, but higher SLEV dissemination rates when exposed first to SLEV than in singly SLEV infected controls. While some mosquitoes became infected with both viruses, only one of those viruses disseminated from the midgut into the legs, indicating a midgut infection barrier to secondary infection. Lower infection rates in mosquitoes exposed to both viruses could change transmission patterns when these viruses are present at epizootic levels.

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“…Reassortment of other bunyaviruses has been demonstrated in vitro in both mosquito and mammalian cells, and in vivo in mosquitoes that are dually infected. 24,30,32,33 In addition, analysis of field isolates suggests that reassortment also can occur in nature. [34][35][36] However, most of these reassortants had been limited to closely related bunyaviruses within a subgroup of the same serogroup, except one report with limited laboratory generated reassortant genotypes between viruses from California encephalitis (CE) subgroup and trivittatus (TVT).…”

Section: Discussionmentioning

confidence: 99%

“…Cytoplasmic RNA was extracted from the cells as previously described. 24 Samples were stored at Ϫ80ЊC until the time of analysis.…”

Section: Methodsmentioning

confidence: 99%

Potential for evolution of California serogroup bunyaviruses by genome reassortment in Aedes albopictus.

Cheng¹,

Rodas²,

Schultz³

et al. 1999

The American Journal of Tropical Medicine and Hygiene

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Abstract. Aedes albopictus was introduced into the United States in used tires in 1985. Its successful colonization of the upper Midwest has potential to alter the current epidemiology of bunyaviruses that circulate in the region. It is permissive for the replication of several arboviruses, including La Crosse (LACV) and Jamestown Canyon (JCV) bunyaviruses. In this study, we demonstrate the ability of LACV and JCV to coinfect Ae. albopictus mosquitoes and to form all six possible reassortant genotypes. All reassortant viruses infect Ae. albopictus orally and can be transmitted to suckling mice. All reassortants are neurovirulent in mice. However, reassortant viruses carrying the LACV M segment in the foreign genetic background of JCV are more neuroinvasive than JCV, or any other reassortant genotype. In addition, these reassortants can replicate in gerbils and infect Ae. triseriatus, characteristics of LACV, but not JCV. Because Ae. albopictus is spreading into new geographic areas and feeds on a variety of mammals, including humans, it has the potential to transmit new, emerging bunyaviruses in nature.

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Heterologous Reassortment of Bunyaviruses in Aedes Triseriatus Mosquitoes and Transovarial and Oral Transmission of Newly Evolved Genotypes

Cited by 30 publications

References 9 publications

Non-random reassortment between the tripartite RNA genomes of La Crosse and snowshoe hare viruses

Non-random reassortment between the tripartite RNA genomes of La Crosse and snowshoe hare viruses

Effect of Sequential Exposure on Infection and Dissemination Rates for West Nile and St. Louis Encephalitis Viruses inCulex quinquefasciatus

Potential for evolution of California serogroup bunyaviruses by genome reassortment in Aedes albopictus.

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