Heterologous overexpression of strawberry bZIP11 induces sugar accumulation and inhibits plant growth of tomato

S1-bZIP Transcription Factors Play Important Roles in the Regulation of Fruit Quality and Stress Response

“…Similar effects such as significantly increased glucose and fructose contents and significantly reduced citric acid content were observed in mutants with enhanced FvebZIP1.1 mORF protein expression due to the uORFs mutation (Xing et al, 2020). In a recent study, heterologous overexpression of strawberry FvbZIP11 affects fruit quality and flavor in tomato (Zhang et al, 2022). In comparison with wild type, the total soluble solid was significantly increased at the breaker, pink and red ripe stages in three transgenic tomato lines.…”

“…The soluble sugar (SS) content was significantly accumulated at 30-50 days after anthesis in transgenic line 6. In addition, the titratable acid content (TTA) was significantly reduced at 30 days after anthesis, while SS/TTA ratio was significantly increased from 20 to 50 days after anthesis in the transgenic tomato line (Zhang et al, 2022). Taken together, these studies demonstrate that the S1-bZIPs play important roles in the regulation of sugar metabolism for quality improvement in plants.…”

“…Remarkably, editing SC-uORF does not severely impair plant growth. The agricultural traits in FvebZIPs1.1 uORF mutants were similar to wild-type (Xing et al, 2020), whereas impaired phenotypes and retarded growth are observed in transgenic lines with the overexpression of AtbZIP11, tbz17, and FvbZIP11 mORF (Ma et al, 2011;Thalor et al, 2012;Zhang et al, 2022). Taken together, this suggests a broad application of editing uORFs of S1-bZIPs for quality improvement in horticultural plants.…”

“…homeostasis in the cells (Wiese et al, 2004;Rahmani et al, 2009). Here, we summarize uORFs of the S1-bZIP subgroup from different horticultural plants, including banana (Hu et al, 2016a), grape (Liu J. et al, 2014), apple (Wang et al, 2015), peach (Wang et al, 2015), cucumber (Baloglu et al, 2014), strawberry (Baloglu et al, 2014;Zhang et al, 2022), petunia The regulated target genes by S1-bZIP (white) or heterodimers of S1-bZIPs and C-bZIPs (gray). Gene names: ASN1, ASPARAGINE SYNTHETASE 1; ProDH, Proline Dehydrogenase; TRE1, Trehalase 1; TPP5/-6, trehalose-phosphate phosphatase 5/-6; SPP, sucrose-phosphatase; SPS1/-2, sucrose-phosphate synthase 1/-2; HXK1, hexokinase 1; DAHPS, 3-Deoxy-D-arabino-heptulosonate 7-phosphate synthase; SK1, shikimate kinase 1; EPSPS, 5-enolpyruvylshikimate 3-phosphate synthase; CS, chorismate synthase; BCAT2, BRANCHED-CHAIN AMINO ACID TRANSAMINASE2; TAT7, TYROSINE AMINOTRANSFERA-SE7; IPT5b, Isopentenyltransferase 5b; NHX1, Na+/H+exchanger 1; COR413-TM1, cold acclimation protein; CBF1/-3, C-repeat/DRE binding factor 1/-3; ADA2b: transcriptional adapter ADA2b; IAA4-1/4-2/-3, INDOL-3-ACETIC ACID INDUCIBLE 4-1/4-2/-3; SHY2, SHORT HYPOCOTYL 2; GH3.3, Indole-3-acetic acid-amido synthetase; NIN88, Defective invertase; 2S2, SEED STORAGE ALBUMIN; CRU3, CRUCIFERIN 3; MAN7, endo-beta-mannase 7; P, phosphorylation.…”

“…There are about four times more bZIP genes in the Arabidopsis genome than in the genomes of other model organisms such as Saccharomyces cerevisiae, Caenorhabditis elegans, and Drosophila melanogaster (Riechmann et al, 2000). Large numbers of bZIP TF family members have been found in many plant species including rice (Nijhawan et al, 2008), maize (Wei et al, 2012), tomato (Li D. et al, 2015), common wheat (Li X. et al, 2015), sorghum (Wang et al, 2011), soybean (Liao et al, 2008), banana (Hu et al, 2016a), cassava (Hu et al, 2016b), grape (Liu J. et al, 2014), peach (Wang et al, 2015), strawberry (Wang et al, 2015;Zhang et al, 2022), apple (Wang et al, 2015;Li et al, 2016), rapeseed (Zhou et al, 2017), radish (Fan et al, 2019), cucumber (Baloglu et al, 2014), tea plant (Xue et al, 2018), sweet potato , watermelon/melon (Unel et al, 2019), Chinese jujube (Zhang et al, 2020a), pepper (Gai et al, 2020), Chinese pear (Manzoor et al, 2021), poplar (Zhao et al, 2021), quinoa (Li et al, 2020) and plum (Li et al, 2021).…”