Heterologous expression of rice 9-cis-epoxycarotenoid dioxygenase 4 (OsNCED4) in Arabidopsis confers sugar oversensitivity and drought tolerance

Hwang, San‐Gwang; Lee, Chia‐Yun; Tseng, Chiu‐yu

doi:10.1186/s40529-018-0219-9

Cited by 45 publications

(29 citation statements)

References 36 publications

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“…Recent study has shown that overexpression of OsNCED3 or OsNCED4 in plants can enhance water stress tolerance by increasing ABA levels [25,45]. In our study, proline accumulated drastically in response to drought and salt stress in OsMADS23-overexpressing lines (Figs 3I and S5H), hinting OsMADS23 might activate the expression of proline synthetic genes such as OsP5CR or OsP5CS1 to modulate stress tolerance.…”

Section: Osmads23 Directly Activates the Transcription Of Osnceds And Osp5cr Through Binding The Carg-box Motifssupporting

confidence: 60%

OsMADS23 phosphorylated by SAPK9 confers drought and salt tolerance by regulating ABA biosynthesis in rice

et al. 2021

PLoS Genet

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Some of MADS-box transcription factors (TFs) have been shown to play essential roles in the adaptation of plant to abiotic stress. Still, the mechanisms that MADS-box proteins regulate plant stress response are not fully understood. Here, a stress-responsive MADS-box TF OsMADS23 from rice conferring the osmotic stress tolerance in plants is reported. Overexpression of OsMADS23 remarkably enhanced, but knockout of the gene greatly reduced the drought and salt tolerance in rice plants. Further, OsMADS23 was shown to promote the biosynthesis of endogenous ABA and proline by activating the transcription of target genes OsNCED2, OsNCED3, OsNCED4 and OsP5CR that are key components for ABA and proline biosynthesis, respectively. Then, the convincing evidence showed that the OsNCED2-knockout mutants had lower ABA levels and exhibited higher sensitivity to drought and oxidative stress than wild type, which is similar to osmads23 mutant. Interestingly, the SnRK2-type protein kinase SAPK9 was found to physically interact with and phosphorylate OsMADS23, and thus increase its stability and transcriptional activity. Furthermore, the activation of OsMADS23 by SAPK9-mediated phosphorylation is dependent on ABA in plants. Collectively, these findings establish a mechanism that OsMADS23 functions as a positive regulator in response to osmotic stress by regulating ABA biosynthesis, and provide a new strategy for improving drought and salt tolerance in rice.

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Section: Osmads23 Directly Activates the Transcription Of Osnceds And Osp5cr Through Binding The Carg-box Motifssupporting

confidence: 60%

OsMADS23 phosphorylated by SAPK9 confers drought and salt tolerance by regulating ABA biosynthesis in rice

et al. 2021

PLoS Genet

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“…ONAC066 was previously found to directly bind to the promoter of OsNCED4 to modulate its expression ( Liu et al, 2018 ). OsNCED4 is one of the 9- cis -epoxycarotenoid dioxygenases acting as a rate-limiting enzyme for abscisic acid biosynthesis ( Huang et al, 2018 , 2019 ; Hwang et al, 2018 ). Considering that ONAC066 is a transcription activator ( Figure 2 ), the binding of ONAC066 to OsNCED4 promoter should drive the expression of OsNCED4 , which in turn leads to ABA biosynthesis.…”

Section: Discussionmentioning

confidence: 99%

ONAC066, A Stress-Responsive NAC Transcription Activator, Positively Contributes to Rice Immunity Against Magnaprothe oryzae Through Modulating Expression of OsWRKY62 and Three Cytochrome P450 Genes

Yuan

Wang

et al. 2021

Front. Plant Sci.

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NAC transcriptional factors constitute a large family in rice and some of them have been demonstrated to play crucial roles in rice immunity. The present study investigated the function and mechanism of ONAC066 in rice immunity. ONAC066 shows transcription activator activity that depends on its C-terminal region in rice cells. ONAC066-OE plants exhibited enhanced resistance while ONAC066-Ri and onac066-1 plants showed attenuated resistance to Magnaporthe oryzae. A total of 81 genes were found to be up-regulated in ONAC066-OE plants, and 26 of them were predicted to be induced by M. oryzae. Four OsWRKY genes, including OsWRKY45 and OsWRKY62, were up-regulated in ONAC066-OE plants but down-regulated in ONAC066-Ri plants. ONAC066 bound to NAC core-binding site in OsWRKY62 promoter and activated OsWRKY62 expression, indicating that OsWRKY62 is a ONAC066 target. A set of cytochrome P450 genes were found to be co-expressed with ONAC066 and 5 of them were up-regulated in ONAC066-OE plants but down-regulated in ONAC066-Ri plants. ONAC066 bound to promoters of cytochrome P450 genes LOC_Os02g30110, LOC_Os06g37300, and LOC_Os02g36150 and activated their transcription, indicating that these three cytochrome P450 genes are ONAC066 targets. These results suggest that ONAC066, as a transcription activator, positively contributes to rice immunity through modulating the expression of OsWRKY62 and a set of cytochrome P450 genes to activate defense response.

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“…Among the upregulated genes in shoots of both rice genotypes, a total of 88 genes were assigned to metabolic pathways. Among these genes, Os07g0154100 and Os08g0445700 , have been reported to be involved in responses to abiotic stress [ 39 – 42 ], with the former encoding 9-cis-epoxycarotenoid dioxygenase, which is a key gene in gibberellic acid biosynthesis, and the latter encoding a stress responsive gene, trehalose-6-phosphate synthase. Expression of OsNCED4 in Arabidopsis increased ABA levels and enhanced tolerance to drought and moderate salt stress [ 39 ], and transgenic rice overexpressing OsTPSs accumulated trehalose, which could confer greater tolerance to cold, high salinity, and drought stress [ 40 – 42 ].…”

Section: Discussionmentioning

confidence: 99%

“…Among these genes, Os07g0154100 and Os08g0445700, have been reported to be involved in responses to abiotic stress [39][40][41][42], with the former encoding 9-cis-epoxycarotenoid dioxygenase, which is a key gene in gibberellic acid biosynthesis, and the latter encoding a stress responsive gene, trehalose-6-phosphate synthase. Expression of OsNCED4 in Arabidopsis increased ABA levels and enhanced tolerance to drought and moderate salt stress [39], and transgenic rice overexpressing OsTPSs accumulated trehalose, which could confer greater tolerance to cold, high salinity, and drought stress [40][41][42]. For the common upregulated genes in roots of both rice genotypes, genes were significantly enriched in metabolic pathways and betalain biosynthesis.…”

Section: Plos Onementioning

confidence: 99%

Comparative transcriptome analysis of two rice genotypes differing in their tolerance to saline-alkaline stress

Tai

et al. 2020

PLoS ONE

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Saline-alkaline stress is an abiotic stress that suppresses rice plant growth and reduces yield. However, few studies have investigated the mechanism by which rice plants respond to saline-alkaline stress at a global transcriptional level. Dongdao-4 and Jigeng-88, which differ in their tolerance to saline-alkaline stress, were used to explore gene expression differences under saline-alkaline stress by RNA-seq technology. In seedlings of Dongdao-4 and Jigeng-88, 3523 and 4066 genes with differential levels of expression were detected, respectively. A total of 799 genes were upregulated in the shoots of both Dongdao-4 and Jigeng-88, while 411 genes were upregulated in the roots of both genotypes. Among the downregulated genes in Dongdao-4 and Jigeng-88, a total of 453 and 372 genes were found in shoots and roots, respectively. Gene ontology (GO) analysis showed that upregulated genes were enriched in several GO terms such as response to stress, response to jasmonic acid, organic acid metabolic process, nicotianamine biosynthetic process, and iron homeostasis. The downregulated genes were enriched in several GO terms, such as photosynthesis and response to reactive oxygen species. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that Dongdao-4 seedlings were specifically enriched in the biosynthesis of secondary metabolites such as diterpenoids and phenylpropanoids. The upregulated genes that were involved in secondary metabolite biosynthesis, amino acid biosynthesis, betalain biosynthesis, organic acid metabolic process, and iron homeostasis pathways may be central to saline-alkaline tolerance in both rice genotypes. In contrast, the genes involved in the diterpenoid and phenylpropanoid biosynthesis pathways may contribute to the greater tolerance to saline-alkaline stress in Dongdao-4 seedlings than in Jigeng-88. These results suggest that Dongdao-4 was equipped with a more efficient mechanism involved in multiple biological processes to adapt to saline-alkaline stress.

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Heterologous expression of rice 9-cis-epoxycarotenoid dioxygenase 4 (OsNCED4) in Arabidopsis confers sugar oversensitivity and drought tolerance

Cited by 45 publications

References 36 publications

OsMADS23 phosphorylated by SAPK9 confers drought and salt tolerance by regulating ABA biosynthesis in rice

OsMADS23 phosphorylated by SAPK9 confers drought and salt tolerance by regulating ABA biosynthesis in rice

ONAC066, A Stress-Responsive NAC Transcription Activator, Positively Contributes to Rice Immunity Against Magnaprothe oryzae Through Modulating Expression of OsWRKY62 and Three Cytochrome P450 Genes

Comparative transcriptome analysis of two rice genotypes differing in their tolerance to saline-alkaline stress

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