Heterologous expression of PDH47 confers drought tolerance in indica rice

Singha, Dhanawantari L.; Tuteja, Narendra; Boro, Dimple; Hazarika, Girindra Nath; Singh, S. S.

doi:10.1007/s11240-017-1248-x

Cited by 16 publications

(2 citation statements)

References 59 publications

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“…However, none of these approaches enhanced tolerance to drought or salt stresses. In addition, previous genetic engineering efforts have reported progress in creating genetically-modified plants with enhanced tolerance to either drought stress [ 73 , 74 ] or salt stress [ 75 , 76 , 77 ], with limited success in conferring tolerance to both drought stress and salt stress in a single transgenic line. In this study, we created genetically modified tobacco plants that had enhanced performance in multiple aspects: photosynthesis, plant growth, drought tolerance, and salt tolerance.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of an Agave Phosphoenolpyruvate Carboxylase Improves Plant Growth and Stress Tolerance

Liu

Zhang

et al. 2021

Cells

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It has been challenging to simultaneously improve photosynthesis and stress tolerance in plants. Crassulacean acid metabolism (CAM) is a CO2-concentrating mechanism that facilitates plant adaptation to water-limited environments. We hypothesized that the ectopic expression of a CAM-specific phosphoenolpyruvate carboxylase (PEPC), an enzyme that catalyzes primary CO2 fixation in CAM plants, would enhance both photosynthesis and abiotic stress tolerance. To test this hypothesis, we engineered a CAM-specific PEPC gene (named AaPEPC1) from Agave americana into tobacco. In comparison with wild-type and empty vector controls, transgenic tobacco plants constitutively expressing AaPEPC1 showed a higher photosynthetic rate and biomass production under normal conditions, along with significant carbon metabolism changes in malate accumulation, the carbon isotope ratio δ13C, and the expression of multiple orthologs of CAM-related genes. Furthermore, AaPEPC1 overexpression enhanced proline biosynthesis, and improved salt and drought tolerance in the transgenic plants. Under salt and drought stress conditions, the dry weight of transgenic tobacco plants overexpressing AaPEPC1 was increased by up to 81.8% and 37.2%, respectively, in comparison with wild-type plants. Our findings open a new door to the simultaneous improvement of photosynthesis and stress tolerance in plants.

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Section: Discussionmentioning

confidence: 99%

Overexpression of an Agave Phosphoenolpyruvate Carboxylase Improves Plant Growth and Stress Tolerance

Liu

Zhang

et al. 2021

Cells

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show abstract

“…The transcripts of PDH47 are upregulated during drought stress that is correlated with increased water status, hydrogen peroxide accumulation and proline accumulation. It also regulates many stress responsive genes present endogenously during drought stress in transgenic rice [38]. There are many more genes that are expressed during the drought stress in different plants and are also potential candidates for producing transgenic rice that are drought tolerant.…”

Section: Rhmyb96mentioning

confidence: 99%