Heterologous Expression of Dehydration-Inducible MfWRKY17 of Myrothamnus Flabellifolia Confers Drought and Salt Tolerance in Arabidopsis

Huang, Zhuo; Guo, Han-Du; Liu, Ling; Jin, Si-Han; Zhu, Peilei; Zhang, Yaping; Jiang, Cai‐Zhong

doi:10.3390/ijms21134603

Cited by 8 publications

(8 citation statements)

References 34 publications

(51 reference statements)

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“…A previous study reported that WRKY17 was involved in conferring stress tolerance in Arabidopsis [ 33 ]. Additionally, The WRKY transcription factors function as positive or negative regulators in salt and drought stress response in different species [ 23 , 34 ]. In the present study, overexpression of EjWRKY17 in Arabidopsis enhanced green cotyledons formation and root elongation under ABA treatment.…”

Section: Discussionmentioning

confidence: 99%

“…The overexpression of GmWRKY16 in Arabidopsis resulted in a water loss rate and reduction of stomatal aperture under drought stress [ 29 ]. In a previous study, MfWRKY17 from Myrothamnus flabellifolia was confirmed as a positive regulator to mediate stomatal closure through an ABA-dependent signaling pathway [ 23 ]. Similarly, EjWRKY17 overexpression in Arabidopsis led to enhanced tolerance to ABA stress and promoted ABA-induced stomatal closure.…”

Section: Discussionmentioning

confidence: 99%

“…AtWRKY57 increased resistance to drought in Arabidopsis through regulation of water loss rate and abscisic acid (ABA) content [ 22 ]. Overexpression of MfWRKY17 in Arabidopsis increased water retention, maintained chlorophyll content, and regulated the transcription levels of ABA biosynthesis and the stress-related genes, thus improving the resistance to drought stress [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

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A WRKY Transcription Factor, EjWRKY17, from Eriobotrya japonica Enhances Drought Tolerance in Transgenic Arabidopsis

Wang

Chen

et al. 2021

IJMS

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The WRKY gene family, which is one of the largest transcription factor (TF) families, plays an important role in numerous aspects of plant growth and development, especially in various stress responses. However, the functional roles of the WRKY gene family in loquat are relatively unknown. In this study, a novel WRKY gene, EjWRKY17, was characterized from Eriobotrya japonica, which was significantly upregulated in leaves by melatonin treatment during drought stress. The EjWRKY17 protein, belonging to group II of the WRKY family, was localized in the nucleus. The results indicated that overexpression of EjWRKY17 increased cotyledon greening and root elongation in transgenic Arabidopsis lines under abscisic acid (ABA) treatment. Meanwhile, overexpression of EjWRKY17 led to enhanced drought tolerance in transgenic lines, which was supported by the lower water loss, limited electrolyte leakage, and lower levels of reactive oxygen species (ROS) and malondialdehyde (MDA). Further investigations showed that overexpression of EjWRKY17 promoted ABA-mediated stomatal closure and remarkably up-regulated ABA biosynthesis and stress-related gene expression in transgenic lines under drought stress. Overall, our findings reveal that EjWRKY17 possibly acts as a positive regulator in ABA-regulated drought tolerance.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A WRKY Transcription Factor, EjWRKY17, from Eriobotrya japonica Enhances Drought Tolerance in Transgenic Arabidopsis

Wang

Chen

et al. 2021

IJMS

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“…The data demonstrated that MfWRKY7 overexpression effectively activated the antioxidant system in transgenic Arabidopsis lines to reduce the ROS-induced oxidative damage and maintain the dynamic balance of ROS, thereby improving tolerance to drought and salinity stresses. Previous studies showed that overexpressing CmWRKY10, SbWRKY30, or MfWRKY17 could enhance the activities of antioxidant enzymes and hence stress tolerance [ 44 , 45 , 46 ]. Therefore, strengthening the antioxidant system might be a generally common strategy of WRKY TFs to increase plant’s tolerance to abiotic stress.…”

Section: Discussionmentioning

confidence: 99%

Heterologous Expression of MfWRKY7 of Resurrection Plant Myrothamnus flabellifolia Enhances Salt and Drought Tolerance in Arabidopsis

Huang

Liu

Jian

et al. 2022

IJMS

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Drought and salinity have become major environmental problems that affect the production of agriculture, forestry and horticulture. The identification of stress-tolerant genes from plants adaptive to harsh environments might be a feasible strategy for plant genetic improvement to address the challenges brought by global climate changes. In this study, a dehydration-upregulated gene MfWRKY7 of resurrection Plant Myrothamnus flabellifolia, encoding a group IId WRKY transcription factor, was cloned and characterized. The overexpression of MfWRKY7 in Arabidopsis increased root length and tolerance to drought and NaCl at both seedling and adult stages. Further investigation indicated that MfWRKY7 transgenic plants had higher contents of chlorophyll, proline, soluble protein, and soluble sugar but a lower water loss rate and malondialdehyde content compared with wild-type plants under both drought and salinity stresses. Moreover, the higher activities of antioxidant enzymes and lower accumulation of O2− and H2O2 in MfWRKY7 transgenic plants were also found, indicating enhanced antioxidation capacity by MfWRKY7. These findings showed that MfWRKY7 may function in positive regulation of responses to drought and salinity stresses, and therefore, it has potential application value in genetic improvement of plant tolerance to abiotic stress.

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“…Studies have shown that overexpression of WRKY transcription factors induces the accumulation of ABA under abiotic stress ( Ma et al, 2019 ; Dong et al, 2020 ). Furthermore, the ABA-responsive genes are significantly up-regulated in plants overexpressing WRKYs under drought and salt stress, indicating that WRKY regulates drought and salt tolerance of plants through ABA regulatory pathway ( Dong et al, 2020 ; Huang et al, 2020 ). Conversely, ABA also induces the expression of WRKYs to mediate stress tolerance.…”

Section: Discussionmentioning

confidence: 99%

WRKY41/WRKY46-miR396b-5p-TPR module mediates abscisic acid-induced cold tolerance of grafted cucumber seedlings

Sun

Chen

et al. 2022

Front. Plant Sci.

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Grafting is one of the key agronomic measures to enhance the tolerance to environmental stresses in horticultural plants, but the specific molecular regulation mechanism in this tolerance largely remains unclear. Here, we found that cucumber grafted onto figleaf gourd rootstock increased cold tolerance through abscisic acid (ABA) activating WRKY41/WRKY46-miR396b-5p-TPR (tetratricopeptide repeat-like superfamily protein) module. Cucumber seedlings grafted onto figleaf gourd increased cold tolerance and induced the expression of miR396b-5p. Furthermore, overexpression of cucumber miR396b-5p in Arabidopsis improved cold tolerance. 5’ RNA ligase-mediated rapid amplification of cDNA ends (5’ RLM-RACE) and transient transformation experiments demonstrated that TPR was the target gene of miR396b-5p, while TPR overexpression plants were hypersensitive to cold stress. The yeast one-hybrid and dual-luciferase assays showed that both WRKY41 and WRKY46 bound to MIR396b-5p promoter to induce its expression. Furthermore, cold stress enhanced the content of ABA in the roots and leaves of figleaf gourd grafted cucumber seedlings. Exogenous application of ABA induced the expression of WRKY41 and WRKY46, and cold tolerance of grafted cucumber seedlings. However, figleaf gourd rootstock-induced cold tolerance was compromised when plants were pretreated with ABA biosynthesis inhibitor. Thus, ABA mediated figleaf gourd grafting-induced cold tolerance of cucumber seedlings through activating the WRKY41/WRKY46-miR396b-5p-TPR module.

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Heterologous Expression of Dehydration-Inducible MfWRKY17 of Myrothamnus Flabellifolia Confers Drought and Salt Tolerance in Arabidopsis

Cited by 8 publications

References 34 publications

A WRKY Transcription Factor, EjWRKY17, from Eriobotrya japonica Enhances Drought Tolerance in Transgenic Arabidopsis

A WRKY Transcription Factor, EjWRKY17, from Eriobotrya japonica Enhances Drought Tolerance in Transgenic Arabidopsis

Heterologous Expression of MfWRKY7 of Resurrection Plant Myrothamnus flabellifolia Enhances Salt and Drought Tolerance in Arabidopsis

WRKY41/WRKY46-miR396b-5p-TPR module mediates abscisic acid-induced cold tolerance of grafted cucumber seedlings

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