Heterologous expression of Chinese wild grapevine VqERFs in Arabidopsis thaliana enhance resistance to Pseudomonas syringae pv. tomato DC3000 and to Botrytis cinerea

Wang, Lan; Liu, Wandi; Wang, Yuejin

doi:10.1016/j.plantsci.2020.110421

Cited by 26 publications

(22 citation statements)

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“…In studies of MdERF11 it was shown to enhance resistance to apple ring rot ( Botryosphaeria dothidea ) via SA signaling pathway [ 35 ], and in those of MdERF3 it was shown to respond to Botrytis cinerea [ 36 ], suggesting that ERFs in apple might enhance defense responses against fungal diseases. Moreover, six ERF genes ( VpERF1 , VpERF2 , VpERF3 , VqERF112 , VqERF114 and VqERF72) from Vitis pseudoreticulata and Vitis quinquangularis showed responses to powdery mildew in different expression patterns [ 37 , 38 ]. These reports showed ERFs have an important function in regulating the defensive response to powdery mildew.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of the Apple (Malus × domestica) MdERF100 in Arabidopsis Increases Resistance to Powdery Mildew

Zhang

Hai

et al. 2021

IJMS

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APETALA2/ETHYLENE RESPONSIVE FACTOR (AP2/ERF) transcription factors play important roles in plant development and stress response. Although AP2/ERF genes have been extensively investigated in model plants such as Arabidopsis thaliana, little is known about their role in biotic stress response in perennial fruit tree crops such as apple (Malus × domestica). Here, we investigated the role of MdERF100 in powdery mildew resistance in apple. MdERF100 localized to the nucleus but showed no transcriptional activation activity. The heterologous expression of MdERF100 in Arabidopsis not only enhanced powdery mildew resistance but also increased reactive oxygen species (ROS) accumulation and cell death. Furthermore, MdERF100-overexpressing Arabidopsis plants exhibited differential expressions of genes involved in jasmonic acid (JA) and salicylic acid (SA) signaling when infected with the powdery mildew pathogen. Additionally, yeast two-hybrid and bimolecular fluorescence complementation assays confirmed that MdERF100 physically interacts with the basic helix–loop–helix (bHLH) protein MdbHLH92. These results suggest that MdERF100 mediates powdery mildew resistance by regulating the JA and SA signaling pathways, and MdbHLH92 is involved in plant defense against powdery mildew. Overall, this study enhances our understanding of the role of MdERF genes in disease resistance, and provides novel insights into the molecular mechanisms of powdery mildew resistance in apple.

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Section: Discussionmentioning

confidence: 99%

Overexpression of the Apple (Malus × domestica) MdERF100 in Arabidopsis Increases Resistance to Powdery Mildew

Zhang

Hai

et al. 2021

IJMS

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“…Our results showed that the silencing of SKIP1b led to increased susceptibility to both Botrytis and Pst DC3000. It consists with previous reports that the silencing of genes will reduce or increase resistance to both Botrytis and Pseudomonas (Li et al, 2015;Wang et al, 2018Wang et al, , 2020.…”

Section: Discussionmentioning

confidence: 96%

SKIP Silencing Decreased Disease Resistance Against Botrytis cinerea and Pseudomonas syringae pv. tomato DC3000 in Tomato

Zhang

Song

et al. 2020

Front. Plant Sci.

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SKIP, a component of the spliceosome, is involved in numerous signaling pathways. However, there is no direct genetic evidence supporting the function of SKIP in defense responses. In this paper, two SKIPs, namely, SlSKIP1a and SlSKIP1b, were analyzed in tomato. qRT-PCR analysis showed that the SlSKIP1b expression was triggered via Pseudomonas syringae pv. tomato (Pst) DC3000 and Botrytis cinerea (B. cinerea), together with the defense-associated signals. In addition, the functions of SlSKIP1a and SlSKIP1b in disease resistance were analyzed in tomato through the virus-induced gene silencing (VIGS) technique. VIGS-mediated SlSKIP1b silencing led to increased accumulation of reactive oxygen species (ROS), along with the decreased expression of defense-related genes (DRGs) after pathogen infection, suggesting that it reduced B. cinerea and Pst DC3000 resistance. There was no significant difference in B. cinerea and Pst DC3000 resistance in TRV-SlSKIP1a-infiltrated plants compared with the TRV-GUS-silencing counterparts. As suggested by the above findings, SlSKIP1b plays a vital role in disease resistance against pathogens possibly by regulating the accumulation of ROS as well as the expression of DRGs.

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“…Arabidopsis plants overexpressing AtERF11 resulted in increased Pst DC3000 resistance, and loss-of-function of AtERF11 decreased Arabidopsis resistance to Pst DC3000 ( Zheng et al, 2019 ). Overexpression of AtERF15 , AcERF2 , VqERF112 , VqERF114 , or VqERF072 in transgenic Arabidopsis showed improved resistance to Pst DC3000 and B. cinerea ( Zhang et al, 2015 ; Sun et al, 2018 ; Wang L. et al, 2020 ). Transgenic Arabidopsis overexpression of MdERF11 led to enhanced resistance against Botryosphaeria dothidea ( Wang J. H. et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…It was also found that AtERF15 is involved in resistance to Pst DC3000 and B. cinerea via the SA and JA/ET signaling pathways ( Zhang et al, 2015 ). VqERF112 , VqERF114 , and VqERF072 acted as positive regulators of plant resistance against Pst DC3000 and B. cinerea through integrating the SA and JA/ET signaling pathways ( Wang L. et al, 2020 ). AtERF11 positively regulated Arabidopsis resistance to Pst DC3000 by directly activating the transcription of AtBT4 , which depends on the SA and ET signaling pathways ( Zheng et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Maize Ethylene Response Factor ZmERF061 Is Required for Resistance to Exserohilum turcicum

et al. 2021

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Plants have evolved a series of sophisticated defense mechanisms to help them from harm. Ethylene Response Factor (ERF) plays pivotal roles in plant immune reactions, however, its underlying mechanism in maize with a defensive function to Exserohilum turcicum (E. turcicum) remains poorly understood. Here, we isolated and characterized a novel ERF transcription factor, designated ZmERF061, from maize. Phylogenetic analysis revealed that ZmERF061 is a member of B3 group in the ERF family. qRT-PCR assays showed that the expression of ZmERF061 is significantly induced by E. turcicum inoculation and hormone treatments with salicylic acid (SA) and methyl jasmonate (MeJA). ZmERF061 was proved to function as a nucleus-localized transcription activator and specifically bind to the GCC-box element. zmerf061 mutant lines resulted in enhanced susceptibility to E. turcicum via decreasing the expression of ZmPR10.1 and ZmPR10.2 and the activity of antioxidant defense system. zmerf061 mutant lines increased the expression of the SA signaling-related gene ZmPR1a and decreased the expression of the jasmonic acid (JA) signaling-related gene ZmLox1 after infection with E. turcicum. In addition, ZmERF061 could interact with ZmMPK6-1. These results suggested that ZmERF061 plays an important role in response to E. turcicum and may be useful in genetic engineering breeding.

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Heterologous expression of Chinese wild grapevine VqERFs in Arabidopsis thaliana enhance resistance to Pseudomonas syringae pv. tomato DC3000 and to Botrytis cinerea

Cited by 26 publications

References 50 publications

Overexpression of the Apple (Malus × domestica) MdERF100 in Arabidopsis Increases Resistance to Powdery Mildew

Overexpression of the Apple (Malus × domestica) MdERF100 in Arabidopsis Increases Resistance to Powdery Mildew

SKIP Silencing Decreased Disease Resistance Against Botrytis cinerea and Pseudomonas syringae pv. tomato DC3000 in Tomato

Maize Ethylene Response Factor ZmERF061 Is Required for Resistance to Exserohilum turcicum

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