Heterologous Expression of Bovine Rhodopsin in<i>Drosophila</i>Photoreceptor Cells

Ahmad, S. Tariq; Natochin, Michael; Barren, Brandy; Artemyev, Nikolai O.; O'Tousa, Joseph E.

doi:10.1167/iovs.06-0281

Cited by 16 publications

(20 citation statements)

References 33 publications

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“…Altogether, these experiments demonstrate that the Rh1 function in promoting rhabdomere and RTW morphogenesis is highly conserved. Because bovine rhodopsin cannot activate the heterotrimeric G proteins (Gqe) involved in invertebrate phototransduction (Ahmad et al, 2006), this also confirms that the Rh1 function in rhabdomere and RTW morphogenesis is independent of heterotrimeric G-protein function.…”

Section: Opsin Function In Rhabdomere Morphogenesis Is Conserved Throsupporting

confidence: 57%

“…3C); however, this base was distorted and small, indicating that human melanopsin does not fully support rhabdomere development. Finally, we expressed the bovine ciliary opsin (rhodopsin) in ninaE l17 eyes (Ahmad et al, 2006). It is known that bovine rhodopsin can also rescue the early developmental failure in rhabdomere morphogenesis (Ahmad et al, 2007), and the corresponding photoreceptors indeed appeared identical to those in wild-type flies 1566 Journal of Cell Science 124 (9) (Fig.…”

Section: Opsin Function In Rhabdomere Morphogenesis Is Conserved Thromentioning

confidence: 97%

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Dynamin- and Rab5-dependent endocytosis is required to preventDrosophilaphotoreceptor degeneration

Pinal

Pichaud

2011

Journal of Cell Science

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In Drosophila photoreceptors, Rhodopsin 1 (ninaE, Rh1) is required for proper morphogenesis and maintenance of the apical light-gathering organelle, the rhabdomere. It has been proposed that Rh1, coupled to the Rho GTPases Rac1 and Cdc42, promotes the morphogenesis of a sub-rhabdomeric F-actin meshwork or rhabdomere terminal web (RTW). The RTW provides mechanical support to the apical microvilli and is likely to guide Rab11-dependent delivery of Rh1-rich membrane to the rhabdomere from the trans Golgi network. However, the nature and function of the molecular pathway involved in RTW morphogenesis remains incomplete. Here, we show that Rh1 function in promoting RTW morphogenesis is light-independent and is conserved throughout evolution. This Rh1 function does not require Gqαe, which is required for phototransduction. Finally, we show that interfering with Dynamin- and Rab5-dependent endocytosis leads to a phenotype that is undistinguishable from that of the ninaE-null mutant. Importantly, the corresponding endocytic activity is essential to prevent early onset of rhabdomere degeneration. In conclusion, we propose that Rh1 function in promoting RTW morphogenesis is not only needed to sustain apical membrane delivery but is also required for proper rhabdomeric membrane endocytosis and turnover.

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Section: Opsin Function In Rhabdomere Morphogenesis Is Conserved Throsupporting

confidence: 57%

Section: Opsin Function In Rhabdomere Morphogenesis Is Conserved Thromentioning

confidence: 97%

Dynamin- and Rab5-dependent endocytosis is required to preventDrosophilaphotoreceptor degeneration

Pinal

Pichaud

2011

Journal of Cell Science

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“…For this analysis, we created Drosophila strains in which a transgene was the only rhodopsin expressed in the R1-6 cells and there was no light response from R7 and R8 photoreceptors (Ahmad et al, 2006). In the absence of a rhodopsin transgene, no light response is evoked using 600-350 nm light stimuli, with intensities adjusted so that similar photon contents were delivered.…”

Section: Aedes Aegypti Aaop8 and Aaop10 Rhodopsins Possess Different mentioning

confidence: 99%

“…Transgenic D. melanogaster expressing A. aegypti Aaop8 and Aaop10 in R1-6 photoreceptors The Aaop8 ORF was directionally cloned into a modified pCaSpeR4 transformation vector, in which the polylinker region was replaced with the Drosophila Rh1 rhodopsin (ninaE) promoter, and a 0.7 kb 3′ untranslated region of the ninaE gene (Ahmad et al, 2006). For Aaop10, we modified the pCaSpeR4 vector by adding an attR1-ccdB-attR2 Gateway recombination site, then the Aaop10 ORF plasmid described above was inserted into this plasmid using Gateway technology (Life Technologies, Grand Island, NY, USA).…”

Section: Production Of Antisera Specific To Mosquito Aaop10 Rhodopsinmentioning

confidence: 99%

Rhodopsin coexpression in UV photoreceptors of Aedes aegypti and Anopheles gambiae mosquitoes

Leming

Whaley

et al. 2013

Journal of Experimental Biology

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Differential rhodopsin gene expression within specialized R7 photoreceptor cells divides the retinas of Aedes aegypti and Anopheles gambiae mosquitoes into distinct domains. The two species express the rhodopsin orthologs Aaop8 and Agop8, respectively, in a large subset of these R7 photoreceptors that function as ultraviolet receptors. We show here that a divergent subfamily of mosquito rhodopsins, Aaop10 and Agop10, is coexpressed in these R7 photoreceptors. The properties of the A. aegypti Aaop8 and Aaop10 rhodopsins were analyzed by creating transgenic Drosophila expressing these rhodopsins. Electroretinogram recordings, and spectral analysis of head extracts, obtained from the Aaop8 strain confirmed that Aaop8 is an ultravioletsensitive rhodopsin. Aaop10 was poorly expressed and capable of eliciting only small and slow light responses in Drosophila photoreceptors, and electroretinogram analysis suggested that it is a long-wavelength rhodopsin with a maximal sensitivity near 500 nm. Thus, coexpression of Aaop10 rhodopsin with Aaop8 rhodopsin has the potential to modify the spectral properties of mosquito ultraviolet receptors. Retention of Op10 rhodopsin family members in the genomes of Drosophila species suggests that this rhodopsin family may play a conserved role in insect vision.

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“…Therefore only the activity of Aaop7 or the Rh1 control rhodopsin was represented in the ERG response (Ahmad et al, 2006). The Aaop7-and the Rh1-expressing Drosophila were then subjected to ERG analysis as described previously (Hu et al, 2014).…”

Section: Spectral Analysis By Erg Recordingsmentioning

confidence: 99%

Expression and light-triggered movement of rhodopsins in the larval visual system of mosquitoes

Rocha

Kimler

Leming

et al. 2015

Journal of Experimental Biology

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During the larval stages, the visual system of the mosquito Aedes aegypti contains five stemmata, often referred to as larval ocelli, positioned laterally on each side of the larval head. Here we show that stemmata contain two photoreceptor types, distinguished by the expression of different rhodopsins. The rhodopsin Aaop3 (GPROP3) is expressed in the majority of the larval photoreceptors. There are two small clusters of photoreceptors located within the satellite and central stemmata that express the rhodopsin Aaop7 (GPROP7) instead of Aaop3. Electroretinogram analysis of transgenic Aaop7 Drosophila indicates that Aaop3 and Aaop7, both classified as longwavelength rhodopsins, possess similar but not identical spectral properties. Light triggers an extensive translocation of Aaop3 from the photosensitive rhabdoms to the cytoplasmic compartment, whereas light-driven translocation of Aaop7 is limited. The results suggest that these photoreceptor cell types play distinct roles in larval vision. An additional component of the larval visual system is the adult compound eye, which starts to develop at the anterior face of the larval stemmata during the 1st instar stage. The photoreceptors of the developing compound eye show rhodopsin expression during the 4th larval instar stage, consistent with indications from previous reports that the adult compound eye contributes to larval and pupal visual capabilities.

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Heterologous Expression of Bovine Rhodopsin inDrosophilaPhotoreceptor Cells

Cited by 16 publications

References 33 publications

Dynamin- and Rab5-dependent endocytosis is required to preventDrosophilaphotoreceptor degeneration

Dynamin- and Rab5-dependent endocytosis is required to preventDrosophilaphotoreceptor degeneration

Rhodopsin coexpression in UV photoreceptors of Aedes aegypti and Anopheles gambiae mosquitoes

Expression and light-triggered movement of rhodopsins in the larval visual system of mosquitoes

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