Abstract:A crucial step in the initiation of cap dependent translation of eukaryotic mRNA is the interaction of the mRNA cap-binding protein, eIF4E, with eIF4G, two integral components of the mRNA cap-binding complex. The present study was aimed to express human eIF4E at high level in E. coli. For high-level expression, the E. coli cells were first transformed with an expression vector that contains the cloned gene, human eIF4E. Following transformation, the recombinant plasmid was purified and stepwise digestion of th… Show more
Set email alert for when this publication receives citations?
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.