Heterologous expression and functional characterization of cysteamine dioxygenase from the deep-sea mussel Bathymodiolus septemdierum

Abstract

In invertebrates inhabiting hydrothermal vent areas, hypotaurine, a precursor of taurine, is thought to mitigate the toxicity of hydrogen sulfide in vent fluids. Information about hypotaurine synthesis pathways in invertebrates is limited, although two pathways, the cysteamine [2-aminoethanethiol (AET)] pathway and the cysteine sulfinate (CSA) pathway are known in mammals. In this study, we cloned a cDNA encoding AET dioxygenase (ADO), the central enzyme of the AET pathway, from the vent mussel Bathymodiolus septemdierum. In the encoded protein (BsADO), functionally important residues, including metal-binding histidines, are conserved. In maximum likelihood phylogenetic analysis, BsADO clustered with ADOs of other invertebrates. By reverse transcription PCR, BsADO mRNA was detected in all tissues examined at similar levels, suggesting that its function is distinct from that of the CSA pathway, predominantly expressed in the gill. BsADO with a His tag, expressed in Escherichia coli in the presence of Fe2+, converted AET to hypotaurine, but BsADO expressed in the absence of iron exhibited lower activity. BsADO was active from pH 8 to 11, and from 0 °C to 37 °C, with a peak at 20 °C. This is the first functional characterization of ADO in marine invertebrates.