Heterologous expression and enzymatic characterization of γ-glutamyltranspeptidase from Bacillus amyloliquefaciens

Lee, Jungmin; Lee, Jaejung; Nam, Gyeong-Hwa; Son, Byung-Sam; Jang, Myoung-Uoon; Lee, So-Won; Hurh, Byung-Serk; Kim, Tae-Jip

doi:10.1007/s12275-017-6638-6

Cited by 20 publications

(26 citation statements)

References 29 publications

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“…Moreover, in addition to the regulatory genes mentioned above, many other genes regulated by SigB ( Lee et al, 2004b ) were also over-expressed in the Δ aor1 mutant compared to the wild-type. These included: catB ( SCO0666 ) ( Cho et al, 2000 ), coding for a catalase that was 11.4-fold up-regulated; trxC , coding for a thioredoxin C (4.7-fold); ssgC ( SCO7289 ) implied in differentiation (8.4-fold); 12 genes of an operon for gas vesicles proteins (GV) ( SCOs6496-6508; Figure 4 , coordinates 7189075-7199188), whose promoter is regulated by SigB and were more than 5-fold up-regulated (Supplementary Table S4 ); and another 15 genes, some of them with an unknown function.…”

Section: Resultsmentioning

confidence: 99%

“…On the other hand, the regulatory network and targets that respond to osmotic stress and heat shock mediated by the master sigma factor SigB, such as SigL, SigM, SigH, SigK, CatB, TrxC, SsgC and a whole gas vesicle (GV) operon ( Lee et al, 2004b , 2005 ; Facey et al, 2011 ), were found up-regulated in the S. coelicolor Δ aor1 mutant strain. The genes encoding WhiB ( SCO3034 ) (indirect target of SigB) and DpsA ( SCO0596 ) (direct target of SigH and SigB) were also differentially expressed and were up-regulated by 2.68-fold and 2.6-fold, respectively.…”

Section: Discussionmentioning

confidence: 99%

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The Orphan Response Regulator Aor1 Is a New Relevant Piece in the Complex Puzzle of Streptomyces coelicolor Antibiotic Regulatory Network

et al. 2017

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Streptomyces coelicolor, the best-known biological antibiotic producer, encodes 29 predicted orphan response regulators (RR) with a putative role in the response to environmental stimuli. However, their implication in relation to secondary metabolite production is mostly unexplored. Here, we show how the deletion of the orphan RR Aor1 (SCO2281) provoked a drastic decrease in the production of the three main antibiotics produced by S. coelicolor and a delay in morphological differentiation. With the aim to better understand the transcriptional events underpinning these phenotypes, and the global role of Aor1 in Streptomyces, a transcriptional fingerprint of the Δaor1 mutant was compared to a wild-type strain. RNA-Seq analysis revealed that the deletion of this orphan regulator affects a strikingly high number of genes, such as the genes involved in secondary metabolism, which matches the antibiotic production profiles observed. Of particular note, the sigma factor SigB and all of the genes comprising its regulon were up regulated in the mutant. Our results show that this event links osmotic stress to secondary metabolite production in S. coelicolor and indicates that the RR encoded by aor1 could be a key regulator in both of these processes.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The Orphan Response Regulator Aor1 Is a New Relevant Piece in the Complex Puzzle of Streptomyces coelicolor Antibiotic Regulatory Network

et al. 2017

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“…TSSs of sig8 , dnaK1 , oxyR , trxA3 , sig22 , and opuBC1 were determined by 5′-RACE analysis of WT gene transcripts under stress conditions (Supplementary Figure S4), leading to the putative -35 and -10 promoter sequences shown in Figure 7 . Analysis of these promoter sequences using the PREDetector web-based program 1 revealed a consensus sequence BGVNVH-N 15 -GSNNHH ( Figure 7 ), which resembles that of σ B -specific promoters of S. coelicolor (GNNTN-N 14-16 -GGGTAY) (Y: C or T) (Lee et al, 2004b). …”

Section: Resultsmentioning

confidence: 99%

“…In S. coelicolor , σ B (Cho et al, 2001; Lee et al, 2004b) and σ K (Mao et al, 2009) are involved in regulation of ACT and RED biosynthesis, although the regulatory mechanisms are unclear. In Streptomyces chattanoogensis L10, alternative σ factor WhiG ch promotes natamycin biosynthesis by directly activating two structural genes, scnC and scnD (Liu S. P. et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

An Alternative σ Factor, σ8, Controls Avermectin Production and Multiple Stress Responses in Streptomyces avermitilis

et al. 2017

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Alternative σ factors in bacteria redirect RNA polymerase to recognize alternative promoters, thereby facilitating coordinated gene expression necessary for adaptive responses. The gene sig8 (sav_741) in Streptomyces avermitilis encodes an alternative σ factor, σ8, highly homologous to σB in Streptomyces coelicolor. Studies reported here demonstrate that σ8 is an important regulator of both avermectin production and stress responses in S. avermitilis. σ8 inhibited avermectin production by indirectly repressing expression of cluster-situated activator gene aveR, and by directly initiating transcription of its downstream gene sav_742, which encodes a direct repressor of ave structural genes. σ8 had no effect on cell growth or morphological differentiation under normal growth conditions. Growth of a sig8-deletion mutant was less than that of wild-type strain on YMS plates following treatment with heat, H2O2, diamide, NaCl, or KCl. sig8 transcription was strongly induced by these environmental stresses, indicating response by σ8 itself. A series of σ8-dependent genes responsive to heat, oxidative and osmotic stress were identified by EMSAs, qRT-PCR and in vitro transcription experiments. These findings indicate that σ8 plays an important role in mediating protective responses to various stress conditions by activating transcription of its target genes. Six σ8-binding promoter sequences were determined and consensus binding sequence BGVNVH-N15-GSNNHH (B: C, T or G, V: A, C or G, S: C or G, H: A, C or T, N: any nucleotide) was identified, leading to prediction of the σ8 regulon. The list consists of 940 putative σ8 target genes, assignable to 17 functional groups, suggesting the wide range of cellular functions controlled by σ8 in S. avermitilis.

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“…Except for σ WhiG , S. coelicolor has nine other alternative σ factors (σ B , σ F , σ G , σ H , σ I , σ K , σ L , σ M , and σ N ), all belonging to the σ B -like σ factors, which are homologs of Bacillus subtilis σ B ( Cho et al, 2001 ; Lee et al, 2004 ). These σ B -like σ factors play important roles in the Streptomyces regulatory system ( Kelemen et al, 1996 ; Kormanec and Sevcikova, 2002 ; Lee et al, 2005 ; Mao et al, 2009 ; Wang et al, 2010 ).…”

Section: Group 3 σ Factorsmentioning

confidence: 99%

Connecting Metabolic Pathways: Sigma Factors in Streptomyces spp.

et al. 2017

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The gram-positive filamentous bacterium Streptomyces is one of the largest resources for bioactive metabolites, particularly antibiotics. Antibiotic production and other metabolic processes are tightly regulated at the transcriptional level. Sigma (σ) factors are components of bacterial RNA polymerases that determine promoter specificity. In Streptomyces, σ factors also play essential roles in signal transduction and in regulatory networks, thereby assisting in their survival in complex environments. However, our current understanding of σ factors in Streptomyces is still limited. In this mini-review, we demonstrate the roles of Streptomyces σ factors, illustrating that these serve as linkers of different metabolic pathways. Further investigations on σ factors may improve our knowledge of Streptomyces physiology and benefit exploitation of Streptomyces resources.

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Heterologous expression and enzymatic characterization of γ-glutamyltranspeptidase from Bacillus amyloliquefaciens

Cited by 20 publications

References 29 publications

The Orphan Response Regulator Aor1 Is a New Relevant Piece in the Complex Puzzle of Streptomyces coelicolor Antibiotic Regulatory Network

The Orphan Response Regulator Aor1 Is a New Relevant Piece in the Complex Puzzle of Streptomyces coelicolor Antibiotic Regulatory Network

An Alternative σ Factor, σ8, Controls Avermectin Production and Multiple Stress Responses in Streptomyces avermitilis

Connecting Metabolic Pathways: Sigma Factors in Streptomyces spp.

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