Heterologous expression and characterization of a high redox potential laccase from Coriolopsis polyzona MUCL 38443

Pinar, Orkun; Tamerler, Candan; Karataş, Ayten Yazgan

doi:10.3906/biy-1605-51

Cited by 14 publications

(8 citation statements)

References 54 publications

(84 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Furthermore, it demonstrated the production of a high concentration of Lac enzymes, regardless of the operating conditions (pH, temperature and incubation time). This finding was not surprising as this isolated fungus belongs to the phylum basidiomycetes, and is known for its enzymatic activities as reported by previous studies ( Chairin et al, 2014 ; Teerapatsakul et al, 2016 ; Pinar et al, 2017 ). In contrast, the zygomycete fungus R. microsporus (belonging to the phylum zygomycota) did not show any positive reactions of the presence of Lac enzymes in its cultures.…”

Section: Discussionsupporting

confidence: 82%

Assessing the Fungal Simultaneous Removal Efficiency of Carbamazepine, Diclofenac and Ibuprofen in Aquatic Environment

et al. 2021

View full text Add to dashboard Cite

Unused pharmaceutical compounds (PhCs) discharged into the aquatic environment have been regarded as emerging pollutants due to potential harmful effects on humans and the environment. Microbial bioremediation is considered as a viable option for their removal from wastewater. The aim of this study was to assess the simultaneous removal of carbamazepine (CBZ), diclofenac (DCF) and ibuprofen (IBP) by previously isolated fungi (Aspergillus niger, Mucor circinelloides, Trichoderma longibrachiatum, Trametes polyzona, and Rhizopus microsporus). The tolerance to PhCs was conducted by tracking the fungal mycelium mat diameters in solid media and its dry biomass in liquid media, at the drug concentration range of 0.1 to 15 mg/L. The fungal enzymatic activities were determined for lignin peroxidase (LiP), manganese peroxidase (MnP) and laccase (Lac), respectively. The PhC removal efficiency of the fungi was assessed in aerated batch flasks and the drug concentrations and intermediate compounds formation were determined by using SPE-UPLC/MS. A tolerance over 70% was recorded for all the fungi at drug concentration of 0.1 mg/L. Manganese peroxidase was produced by all the fungi with very low amount of LiP, while all the enzymes were produced by T. polyzona. The pH of 4.3, temperature 37 ± 1.5°C and incubation time of 6 days were the optimum parameters for the fungal enzymatic activities. The best removal of CBZ (87%) was achieved by R. microsporus after 10 days. Between 78 and 100% removal of DCF was observed by all the fungi after 24 h, while 98% of IBP was removed after 2 days by M. circinelloides. Only a few intermediate compounds were identified after 3 days and disappeared after 10 days of incubation. This study demonstrated that apart from the basidiomycetes, the ascomycetes and zygomycetes are also producers of ligninolytic enzymes and have the ability to biodegrade emerging pollutants such as PhCs.

show abstract

Section: Discussionsupporting

confidence: 82%

Assessing the Fungal Simultaneous Removal Efficiency of Carbamazepine, Diclofenac and Ibuprofen in Aquatic Environment

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The laccase of genus Trametes has been extensively studied, and Trametes hirsuta Han 726 used in this study also belongs to one species of genus Trametes (Kołodziejczak-Radzimska et al 2020;Sun et al 2020;Bilal et al 2021;Mejía-Otálvaro et al 2021;Navada and Kulal 2021;Wulandari et al 2021;Zhang et al 2021). However, the laccase of genus Coriolopsis has been less studied, and previous studies were mainly focused on recombinant expression, heterologous expression, and the selection of high production of laccase (Songulashvili et al 2016;Xu et al 2016;Avelar et al 2017;Pinar et al 2017;Glazunova et al 2018). Additionally, lignocellulosic biomass used in previous studies merely related to laccase from genus Coriolopsis were chestnut shell and barley bran.…”

Section: Statistical Analysis Resultsmentioning

confidence: 99%

Laccase activities from three white-rot fungal species isolated from their native habitat in North China using solid-state fermentation with lignocellulosic biomass

Liu

Zhao

et al. 2022

BioRes

View full text Add to dashboard Cite

Laccase has huge potential application in all aspects of biotechnology due to the ability of laccase to oxidize a wide range of phenolic and non-phenolic compounds. The future success of such applications requires large amounts of laccase with low costs. White-rot fungi are important groups of laccase production. In this study, three white-rot fungi, Phlebia acerina Han 618, Trametes hirsuta Han 726, and Coriolopsis trogii Han 751, isolated from a native North China habitat, were identified by the method of molecular biology and preliminary screening of the ability of laccase-production by guaiacol selection medium. Then they were fermented on different lignocellulosic biomass. Three species showed consistency in preference of lignocellulosic biomass, and the presence of stalk of Sorghum bicolor was more suitable for secreting laccase. The capacity of laccase secretion from different species was significantly different. The capacity of secreting laccase of C. trogii Han 751 was superior to that of P. acerina Han 618 and T. hirsuta Han 726. The discovery of a new strain with superior capacity of secreting laccase and suitable lignocellulosic biomass were helpful for laying a foundation for the optimization of the fermentation conditions for the highest laccase production, the isolation and purification of laccase, and the industrial application of laccase.

show abstract

“…The addition of 0.8 mM CuSO 4 increased the activity from 211.29 U/L to 380.52 U/L. Moreover, further optimization, including the concentration of methanol and alanine in the medium and the temperature of the culture, increased the activity of heterologous laccase to a maximum value of 800 U/L [32]. In turn, Bao et al [33] achieved the expression of laccase Lac1 from Coprinus comatus in P. pastoris at the level of activity of 550 U/L in a medium containing 0.5 mM Cu 2+ and 1% methanol [33].…”

Section: Figurementioning

confidence: 99%

Novel Cold-Adapted Recombinant Laccase KbLcc1 from Kabatiella bupleuri G3 IBMiP as a Green Catalyst in Biotransformation

Wiśniewska

Twarda-Cłapa

Białkowska

2021

IJMS

View full text Add to dashboard Cite

Cold-adapted enzymes are useful tools in the organic syntheses conducted in mixed aqueous-organic or non-aqueous solvents due to their molecular flexibility that stabilizes the proteins in low water activity environments. A novel psychrophilic laccase gene from Kabatiella bupleuri, G3 IBMiP, was spliced by Overlap‑Extension PCR (OE-PCR) and expressed in Pichia pastoris. Purified recombinant KbLcc1 laccase has an optimal temperature of 30 °C and pH of 3.5, 5.5, 6.0, and 7.0 in the reaction with 2,2′‑azino‑bis(3‑ethylbenzothiazoline‑6‑sulfonic acid) (ABTS), guaiacol, sinapic acid, and syringaldazine, respectively. Moreover, laccase KbLcc1 is highly thermolabile, as it loses 40% of activity after 30 min at 40 °C and is inactivated at 50 °C after the same period of incubation. The new enzyme remained active with 1 mM of Ni2+, Cu2+, Mn2+, and Zn2+ and with 2 mM of Co2+, Ca2+, and Mg2+, but Fe2+ greatly inhibited the laccase activity. Moreover, 1% ethanol had no impact on KbLcc1, although acetone and ethyl acetate decreased the laccase activity. The presence of hexane (40%, v/v) caused a 58% increase in activity. Laccase KbLcc1 could be applied in the decolorization of synthetic dyes and in the biotransformation of ferulic acid to vanillin. After 5 days of reaction at 20 °C, pH 3.5, with 1 mM ABTS as a mediator, the vanillin concentration was 21.9 mg/L and the molar yield of transformation reached 14.39%.

show abstract

Heterologous expression and characterization of a high redox potential laccase from Coriolopsis polyzona MUCL 38443

Cited by 14 publications

References 54 publications

Assessing the Fungal Simultaneous Removal Efficiency of Carbamazepine, Diclofenac and Ibuprofen in Aquatic Environment

Assessing the Fungal Simultaneous Removal Efficiency of Carbamazepine, Diclofenac and Ibuprofen in Aquatic Environment

Laccase activities from three white-rot fungal species isolated from their native habitat in North China using solid-state fermentation with lignocellulosic biomass

Novel Cold-Adapted Recombinant Laccase KbLcc1 from Kabatiella bupleuri G3 IBMiP as a Green Catalyst in Biotransformation

Contact Info

Product

Resources

About