Heterologous Expression and Biochemical Analysis Reveal a Schizokinen-Based Siderophore Pathway in
            <i>Leptolyngbya</i>
            (Cyanobacteria)

Wang, Suqin; Pearson, Leanne A.; Mazmouz, Rabia; Liu, Tianzhe; Neilan, Brett A.

doi:10.1128/aem.02373-21

Cited by 7 publications

(5 citation statements)

References 36 publications

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“…Moreover, the presence of terpene and arylpolyene clusters suggests the capacity for volatile compound production and epiphytic survival, respectively, which may contribute to plant growth promotion through enhancing nutrient uptake and stimulating plant defences (He et al, 2020; Singh and Sharma, 2015). Notably, the siderophore BGCs indicate the ability of the novel Luteibacter species to facilitate iron acquisition, a crucial factor in plant health and growth (Wang et al, 2022). Finally, the transAT-PKS clusters hint at the potential production of complex polyketides with diverse biological activities (Almeida et al, 2022), which can further contribute to both plant protection and growth promotion.…”

Section: Resultsmentioning

confidence: 99%

Luteibacter sahniisp. nov., a novel yellow-pigmented probiotic bacterium from rice seed microbiome

Jaiswal,

Rana,

Nayak

et al. 2023

Preprint

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The genusLuteibacter, a member of the familyRhodanobacteraceae, encompasses Gram-negative bacteria found in diverse environments. In the present study, four yellow-pigmented bacterial isolates designated as PPL193T, PPL201, PPL552, and PPL554 were obtained and identified as Gram-negative, rod-shaped, and motile bacteria. Biochemical characterization and examination of the 16S rRNA gene sequence, derived from the genomic sequence, identified it as belonging to the genusLuteibacter. The isolates are closely related toLuteibacter yeojuensisR2A16-10T, forming a distinct monophyletic lineage withL. aegosomatisKCTC 92392TandL. anthropiCCUG25036T. The calculated values for pairwise ortho Average Nucleotide Identity and digital DNA-DNA hybridization in comparison to previously reportedLuteibacterspecies fell below the established thresholds for species delineation. As this novel species was isolated from rice seeds as a potentialXanthomonasdue to its distinctive yellow-colored colonies, we sought to identify the presence of xanthomonadin pigment in this species. Intriguingly, our findings revealed the presence of the typical peak corresponding to xanthomonadin in the UV spectra, confirming its presence in this novel species and adaptation to plant habitat. Furthermore, the detailed genomic investigation also uncovered the genomic locus corresponding to xanthomonadin biosynthetic gene cluster, further suggesting that members of this novel species are co-habitants of plant pathogenic and plant probioticXanthomonasgroup of phytobacteria within rice seeds. Apart from protease production, the species was found to produce Indole-3-Acetic Acid (IAA) in higher quantities and was also able to protect plants fromXanthomonas oryzaepv.oryzae, a major pathogen of rice indicating its probiotic nature. Genome scanning revealed the presence of genomic region(s) encoding loci for biosynthesis of anti-microbial peptides and other metabolites with probiotic properties, further confirming its probiotic properties. This study highlights the importance of using a combination of phenotypic and genotypic methods for bacterial identification and expands our knowledge of the diversity and distribution of diverse bacteria associated with rice seeds and their microbiome.Luteibacter sahniisp. nov. is proposed as a novel species of the genusLuteibacterwith PPL193T=MTCC 13290T=ICMP 24807T=CFBP 9144Tas its type strain and PPL201, PPL552, and PPL554 as other constituent members.

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Section: Resultsmentioning

confidence: 99%

Luteibacter sahniisp. nov., a novel yellow-pigmented probiotic bacterium from rice seed microbiome

Jaiswal,

Rana,

Nayak

et al. 2023

Preprint

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“…Out of all the four complete genome assemblies on the NCBI database, MGMM1 revealed a cluster for the production of the cationic lipopeptide laterocidine, which is produced by Brevibacillus species and has a bacteriocidal effect against the antibiotic-resistant pathogens of the “ESKAPE” group [ 76 ]. Another common siderophore, schizokinen, is found in all four compared genomes of B. glycinifermentans , which has been reported to be a potent iron-chelating siderophore produced by cyanobacteria [ 77 ]. The presence of these genes does not directly correlate to their expression, which can be observed through the presence of the phytase gene but is phenotypically absent.…”

Section: Discussionmentioning

confidence: 99%

Characterization of a Novel Bacillus glycinifermentans Strain MGMM1 Based on Full Genome Analysis and Phenotypic Properties for Biotechnological Applications

Afordoanyi,

Diabankana,

Komissarov

et al. 2023

Microorganisms

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Bacillus species have gained much attention based on their phenotypic characteristics and their genetic architecture as biological control agents and plant growth-promotor with bioremediation potential. In this study, we analyzed the whole genome of a novel strain, Bacillus glycinifermentans MGMM1, isolated from the rhizosphere of a weed plant (Senna occidentalis) and assayed its phenotypic characteristics, as well as antifungal and biocontrol ability. The whole genome analysis of MGMM1 identified 4259 putative coding sequences, with an encoding density of 95.75% attributed to biological functions, including genes involved in stimulating plant growth, such as acetolactate synthase, alsS, and genes involved in the resistance to heavy metal antimony (arsB and arsC). AntiSMASH revealed the presence of biosynthetic gene clusters plipastatin, fengycin, laterocidine, geobacillin II, lichenysin, butirosin A and schizokinen. Tests in vitro confirmed that MGMM1 exhibited antifungal activity against Fusarium oxysporum f.sp. radicis-lycopersici (Forl) ZUM2407, Alternaria alternata, F. graminearum and F. spp. and produce protease, lipase amylase and cellulase. Bacillus glycinifermentans MGMM1 demonstrated proteolytic (4.82 ± 1.04 U/mL), amylolytic (0.84 ± 0.05 U/mL) and cellulosic (0.35 ± 0.02 U/mL) enzymatic activities, as well as indole-3-acetic acid production (48.96 ± 1.43 μg/mL). Moreover, the probiotic strain MGMM1 demonstrated a high biocontrol potential of inhibiting (up to 51.45 ± 8.08%) the development of tomato disease caused by Forl ZUM2407. These results suggest that B. glycinifermentans MGMM1 has significant potential as a biocontrol, plant growth-promoting agent in agriculture.

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“…Certain strains may be known producers of siderophores but the corresponding genes are not known. On the contrary, certain biosynthetic gene clusters may have been identified by genome mining strategies but the produced siderophore has not yet been characterized [ 87 , 88 ]. Heterologous expression of single genes identified from BGCs is a powerful strategy to characterize the encoded enzymes [ 89 ].…”

Section: Synthetic and Heterologous Pathwaysmentioning

confidence: 99%

Engineering Siderophore Biosynthesis and Regulation Pathways to Increase Diversity and Availability

2023

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Siderophores are small metal chelators synthesized by numerous organisms to access iron. These secondary metabolites are ubiquitously present on Earth, and because their production represents the main strategy to assimilate iron, they play an important role in both positive and negative interactions between organisms. In addition, siderophores are used in biotechnology for diverse applications in medicine, agriculture and the environment. The generation of non-natural siderophore analogs provides a new opportunity to create new-to-nature chelating biomolecules that can offer new properties to expand applications. This review summarizes the main strategies of combinatorial biosynthesis that have been used to generate siderophore analogs. We first provide a brief overview of siderophore biosynthesis, followed by a description of the strategies, namely, precursor-directed biosynthesis, the design of synthetic or heterologous pathways and enzyme engineering, used in siderophore biosynthetic pathways to create diversity. In addition, this review highlights the engineering strategies that have been used to improve the production of siderophores by cells to facilitate their downstream utilization.

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Heterologous Expression and Biochemical Analysis Reveal a Schizokinen-Based Siderophore Pathway in Leptolyngbya (Cyanobacteria)

Abstract: Iron availability limits the growth of many microorganisms, particularly those residing in high nutrient–low chlorophyll aquatic environments. Therefore, characterizing iron acquisition pathways in phytoplankton is essential for understanding nutrient cycling in our oceans.

Cited by 7 publications

References 36 publications

Luteibacter sahniisp. nov., a novel yellow-pigmented probiotic bacterium from rice seed microbiome

Luteibacter sahniisp. nov., a novel yellow-pigmented probiotic bacterium from rice seed microbiome

Characterization of a Novel Bacillus glycinifermentans Strain MGMM1 Based on Full Genome Analysis and Phenotypic Properties for Biotechnological Applications

Engineering Siderophore Biosynthesis and Regulation Pathways to Increase Diversity and Availability

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