“…Considering that these three genes were adjacent to the DvVS gene, we co-expressed them with DvVS and vndE to further verify their functionalities. As many fungal biosynthetic pathways are revealed through heterologous expression of A. oryzae [ 17 , [39] , [40] , [41] , [42] ], these genes were cloned into the expression vectors pUSA2 and pUARA2 for heterologous expression in A. oryzae . Two expression plasmids, pUSA2- orfABC and pUARA2- vndDE , were prepared and transformed into A. oryzae to generate AO- orfABC/vndDE .…”