Heteroleptic N,N-donor pyrazole based Pt(II) and Pd(II) complexes: DNA binding, molecular docking and cytotoxicity studies

Patel, Nikita; Bhatt, Bhupesh S.; Patel, Mohan N.

doi:10.1016/j.ica.2019.119130

Cited by 43 publications

(12 citation statements)

References 44 publications

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“…All synthesized compounds were tested for MIC using the broth dilution method. The method used to evaluate MIC value is according to the reported process [25]. Serially two-fold dilution of the test compound added to three Gram(− ve) microorganisms, namely Pseudomonas aeruginosa (MTCC P-09), Escherichia Coli (MTCC 433), Serratia marcescens (MTCC 7103) and two Gram(+ ve) bacteria, namely Bacillus subtilis Staphylococcus aureus (MTCC 3160), (MTCC 7193).…”

Section: Biological Screening Of Compound 251 Mic By Broth Dilution Methodsmentioning

confidence: 99%

“…The cytotoxicity of complexes was studied on brine shrimp, Artemia cysts, according to the method reported by Meyer et al [25].…”

Section: Evaluation Of In Vitro Cytotoxicitymentioning

confidence: 99%

“…Graphical representation of MIC value of ligand(L 1 -L 6 ) and Pd(II) complexes(I-VI) is shown in Fig. 1 The higher antimicrobial activity (lower MIC value) of the metal complexes (MIC = 45-105 µM) compared to the metal salt (MIC > 1000 µM) and respective ligand is due to nuclearity of the metal center in the complexes, chelate effects, nature of the ligands, and ion neutralizing the complexes [25].…”

Section: In Vitro Antibacterialmentioning

confidence: 99%

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Heteroleptic C, N- Donor Pd(II) Complexes: Synthesis, Characterization, DNA/BSA Binding Interactions And Biological Studies

Patel

Dhaduk

Dabhi

et al. 2021

Preprint

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A series of trisubstituted pyrazole-based ligands (L1-L6) and corresponding palladium(II) complexes were synthesized and characterized by conductivity measurement, 1H NMR, 13C NMR, Fourier Transform infrared (FT-IR) spectroscopy and liquid chromatography-mass spectrometry (LC-MS). Synthesized compounds were screened for various biological activities. UV-Vis spectroscopy, viscosity measurement, fluorescence spectroscopy and molecular docking studies were used to determine the binding mode between HS-DNA and complexes, which suggest intercalation mode of binding. The protein binding study of complexes was evaluated by UV-visible spectroscopy. Antibacterial study of the complexes was screened against two Gram (+ve) and three Gram (-ve) bacteria and results show that all complexes are more effective against microorganisms than their respective ligands. The cytotoxicity of the synthesized compounds was tested against brine shrimp and MCF-7 Cells. The LC50 values of the ligand and complexes were found in the range of 9.24-4.12 µg/mL and 5.68-7.94 µg/mL, respectively.

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Section: Biological Screening Of Compound 251 Mic By Broth Dilution Methodsmentioning

confidence: 99%

“…The cytotoxicity of complexes was studied on brine shrimp, Artemia cysts, according to the method reported by Meyer et al [25].…”

Section: Evaluation Of In Vitro Cytotoxicitymentioning

confidence: 99%

Section: In Vitro Antibacterialmentioning

confidence: 99%

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Heteroleptic C, N- Donor Pd(II) Complexes: Synthesis, Characterization, DNA/BSA Binding Interactions And Biological Studies

Patel

Dhaduk

Dabhi

et al. 2021

Preprint

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“…The magnitude of this shift can be estimated ChemistrySelect by using the degree of the ligand's interaction with the DNA structure. [49,50] Electronic transitions are mainly seen due to chromophoric groups in the purines and pyrimidines present in DNA. The spectrum tends to show a maximum absorption in the 200-250 nm region.…”

Section: Absorption Spectroscopymentioning

confidence: 99%

In vitro Analytical Techniques as Screening Tools to investigate the Metal chelate‐DNA interactions

et al. 2023

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Deoxyribose nucleic acid (DNA) is found to be the most efficient pharmacological target of many synthetic molecules which are deemed as potential drugs with clinical applications. DNA binding agents are known to regulate several cell functions (gene expression and replication) by adopting various protocols which include the annihilation of the cell membrane, interruption in protein synthesis, and irreversible binding to cell receptors. Recently, several studies have explored fundamental aspects of drug-DNA interactions, providing new insights into the driving forces that render the formation of the drug-DNA complex. In order to study and understand these biologically important reaction mechanisms, several screening tools have been devised and the specificity of drug molecules binding to DNA were studied in detail. This review will discuss the utilization of various analytical tools which include UV-vis spectroscopy, fluorescence spectroscopy, circular dichroism, viscosity measurement, Raman spectroscopy, cyclic voltammetry, and DNA fragmentation assay used for studying drug binding mode and the mechanism involved.

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“…For instance, Pd( ii ) and Pt( ii ) analogues with quinoline and morpholine ligands revealed that Pd( ii ) complexes were more effective as anticancer agents and also acted as antibacterial agents. 8 Pd( ii ) coordinated by two cis chloride ligands and a bidentate ligand was found to cross the cell membrane and interact with DNA and/or with proteins in the cytoplasm. 9 Overall, Pd( ii ) complexes have chemical characteristics and properties similar to (or even better than) Pt( ii ) complexes, with a mechanism of action as anticancer drugs that is often related to their ability to interact with DNA (even if not exclusively) via binding modes that are mainly non-covalent, among which intercalation plays the main role.…”

Section: Introductionmentioning

confidence: 99%

A biophysical study of the interactions of palladium(ii), platinum(ii) and gold(iii) complexes of aminopyridyl-2,2′-bipyridine ligands with RNAs and other nucleic acid structures

et al. 2023

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Heteroleptic N,N-donor pyrazole based Pt(II) and Pd(II) complexes: DNA binding, molecular docking and cytotoxicity studies

Cited by 43 publications

References 44 publications

Heteroleptic C, N- Donor Pd(II) Complexes: Synthesis, Characterization, DNA/BSA Binding Interactions And Biological Studies

Heteroleptic C, N- Donor Pd(II) Complexes: Synthesis, Characterization, DNA/BSA Binding Interactions And Biological Studies

In vitro Analytical Techniques as Screening Tools to investigate the Metal chelate‐DNA interactions

A biophysical study of the interactions of palladium(ii), platinum(ii) and gold(iii) complexes of aminopyridyl-2,2′-bipyridine ligands with RNAs and other nucleic acid structures

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