Heterogenous distribution of fibronectin, tenascin-c, and laminin immunoreactive material in the cumulus-coronal cells surrounding mature human oocytes from IVF-ET protocols—Evidence that they are composed of different subpopulations: An immunohistochemical study using scanning confocal laser and fluorescence microscopy

Familiari, Giuseppe; Verlengia, Cristina; Nottola, Stefania Annarita; Renda, T; Micara, Giulietta; Aragona, Cesare; Zardi, Luciano; Motta, Pietro M.

doi:10.1002/(sici)1098-2795(199603)43:3<392::aid-mrd14>3.0.co;2-3

Cited by 32 publications

(16 citation statements)

References 51 publications

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“…Several glycoproteins, which are potential ligands for the ciliary crown, have been identified in cumulus matrix and include inter-␣-trypsin inhibitor [14], a dermatan sulfate proteoglycan, and a 46-kDa protein [15]. In addition, immunohistochemical data suggest that proteins known to influence adhesion, such as fibronectin, tenascin-C, and laminin, are located in the extracellular matrix of human OCCs [16]. The oviductal ciliary crown has not yet been characterized biochemically, but pretreatment of cilia with neuraminidase abolishes pick-up, apparently by altering the crown; this suggests that sialic acid residues in the crown participate in adhesion [7,8].…”

Section: Discussionmentioning

confidence: 99%

“…The adhesive partner on the infundibular surface could be the secretory cell plasma membranes, the entire ciliary membrane, or a restricted area of the ciliary membrane, such as the ciliary crown. Within the OCC, either the cells or the matrix or both could adhere to the oviductal epithelium, and if the matrix is involved, adhesion could occur via either hyaluronic acid [13] or matrix proteins [14][15][16]. Prior experiments suggest that altering adhesion affects OCC pick-up [7,8], but no assay has existed to quantitatively measure adhesion between the OCC and infundibulum, nor have quantitative studies been done to show how altering adhesion changes pick-up.…”

Section: Introductionmentioning

confidence: 99%

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Assay and Importance of Adhesive Interaction Between Hamster (Mesocricetus auratus) Oocyte-Cumulus Complexes and the Oviductal Epithelium1

Lam

Gieseke

Knöll

et al. 2000

Biology of Reproduction

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Adhesion between the oocyte-cumulus complex and infundibulum plays an important, but poorly understood, role in oocyte pick-up. The purposes of this study were to determine which components of the oocyte-cumulus complex and oviductal epithelium function in adhesion, to measure adhesion under physiological conditions, and to examine the effect of modulation of adhesion on oocyte-cumulus complex pick-up rate. Oocyte-cumulus complexes containing an expanded matrix were readily transported into the oviduct, while unexpanded complexes lacking an extracellular matrix were not picked up, indicating that the matrix is necessary for pick-up. Transmission electron microscopy revealed that during pick-up, adhesion occurred specifically between the ciliary crowns of the oviduct and the granules and filaments of the cumulus matrix. An assay was developed using vacuum from a low-flow peristaltic precision pump, modified for bi-directional flow, to measure the strength of adhesion between the oocyte-cumulus complex and the oviductal epithelium, and adhesion was measured during physiological conditions. The lectin wheat germ agglutinin and the polycation poly-L-lysine were then used to modulate adhesion, and the effects of increasing or decreasing adhesion on oocyte pick-up rate and ciliary beat frequency were examined. The data show that 1) the matrix of the oocyte-cumulus complex and the ciliary crowns of the oviduct function in adhesion during pick-up and that adhesion is necessary for pick-up, 2) adhesion can be assayed quantitatively and is very uniform among control infundibula, and 3) decreasing or increasing adhesion decreases oocyte pick-up rate and in some cases prevents pick-up without affecting ciliary beat frequency.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Assay and Importance of Adhesive Interaction Between Hamster (Mesocricetus auratus) Oocyte-Cumulus Complexes and the Oviductal Epithelium1

Lam

Gieseke

Knöll

et al. 2000

Biology of Reproduction

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“…While binding to HA at their Nterminal domains, the HA-binding proteoglycan family molecules could also bind at their C-terminal lectin domains to other matrix molecules, such as tenascin-C, and form extracellular network structures in tissues like brain and cartilage (Yamaguchi, 2000). Tenascin-C was also found in the cumulus oophorus matrix, suggesting the involvement of such molecular interaction in the construction of cumulus oophorus matrices (Familiari et al, 1996). In addition, the expression of TSG-6 (tumor necrosis factor-stimulated gene-6) gene, which codes for a 35 kDa glycoprotein containing a HA-binding domain, was shown to be specifically upregulated during cumulus oophorus expansion (Fulop et al, 1997b).…”

Section: The Cumulus Oophorus Matrix Is a Hyaluronanrich Matrixmentioning

confidence: 99%

“…In contrast, the presence of inhibitors that block the matrix deposition and consequently cumulus oophorus expansion did not affect the cytoskeletonal redistribution, indicating that the cytoskeletonal rearrangement was a prerequisite for the matrix deposition. Microfilaments might affect the deposition of type IV collagen, laminin and fibronectin through actin-linked membrane receptor integrin =6>1 (Sutovsky et al, 1995;Familiari et al, 1996). Extrafollicular factors are also required for the construction of the cumulus oophorus matrix ( Fig.…”

Section: The Cumulus Oophorus Matrix Is a Hyaluronanrich Matrixmentioning

confidence: 99%

Cumulus Oophorus Extracellular Matrix: Its Construction and Regulation.

Zhuo

Kimata

2001

Cell Struct. Funct.

100

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ABSTRACT. Cumulus oophorus, an investing structure unique to oocytes of higher mammals, is induced to synthesize an extensive extracellular matrix by ovulatory stimulus, leading to the characteristic preovulatory expansion of the cumulus-oocyte complex. The extracellular matrix consists of cumulus cell-secreted hyaluronan, proteoglycans and proteins, as well as extrafollicularly originated SHAPs (serum-derived hyaluronan-associated proteins) that are bound covalently to hyaluronan. The secretion and assembly of matrix molecules by cumulus cells are temporally regulated by factors derived from both mural granulosa cells and oocyte, which synchronize the deposition of the cumulus oophorus matrix with other intrafollicular ovulatory events. The cumulus oophorus matrix is essential for ovulation and subsequent fertilization. Recently, taking advantage of animal models with defined genetic modifications, it has become possible to investigate in vivo the structure of the cumulus oophorus matrix, the regulatory mechanism for matrix deposition and its biological functions. This review focuses on the recent findings on the construction of the cumulus oophorus matrix and the regulation.Key words: extracellular matrix/hyaluronan/SHAP-HA complex/proteoglycan/ovulation/fertilization During late stages of folliculogenesis in higher mammals, a population of follicular granulosa cells forms a specific cumulus oophorus structure in the periphery of the ovum. In mice, a cumulus oophorus structure consists of about 3,000-5,000 cumulus cells that are held together by a gap junction network and subsequently by an extensive extracellular matrix after the physiological ovulatory stimulation of luteinizing hormone (LH) surge. The preovulatory deposition of cumulus oophorus matrix causes a characteristic volumetric enlargement of the cumulus-oocyte complex (COC) (about 20-40 times the initial volume) and dissociation of COC from the follicle wall, accompanied by the resumption of oocyte meiosis that is characterized by germinal vesicle breakdown and extrusion of the first polar body. At the same time, LH surge also stimulates active steroidogenesis, prostaglandin/leukotriene synthesis and matrix-degrading protease secretion in mural granulosa cells and theca cells, and great changes in the permeability of the follicle-blood barriers, leading to accumulation of follicle fluid and increase of intrafollicular pressure. As a result, the follicle wall ruptures to extrude COC. The expanded cumulus oophorus structures often remain investing the oocytes in oviducts until several hours after fertilization (Fig. 1). The cumulus oophorus structure is thus thought to play important roles in oocyte development, ovulation and fertilization in higher mammals. The cumulus oophorus matrix is a hyaluronanrich matrixThe cumulus oophorus matrix is rich in hyaluronan (HA) as shown by studies on HA synthesis (Salustri et al., 1989), sensitivity to hyaluronidase (Talbot et al., 1984), and inhibition of matrix formation by HA oligomers or HA synthesis inhibito...

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“…Considering these results and the values that have been reported in the literature, we decided to perform the following experiments using 10 lg/ml Ln because this concentration is similar to that reported to be present in the cumulus oophorus matrix [43,44].…”

Section: Effect Of Ln On the Armentioning

confidence: 74%

The Laminin-Induced Acrosome Reaction in Human Sperm Is Mediated by Src Kinases and the Proteasome1

Tapia

Rojas

Morales

et al. 2011

Biology of Reproduction

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The aim of this work was to determine whether laminin (Ln), an extracellular matrix protein, induces the intracellular events that may be involved in producing the acrosome reaction in human sperm. To this end, we evaluated the effect of Ln on tyrosine phosphorylation, intracellular calcium concentration, proteasome activity, and phosphorylation in human sperm. Aliquots of highly motile sperm selected with a Percoll gradient, were incubated with different concentrations of Ln (0-20 μg/ml) for different periods (0-18 h). The percentage of viable acrosome-reacted sperm was evaluated using fluorescein isothiocyanate-labeled Pisum sativum agglutinin and Hoechst 33258 DNA dye. Tyrosine phosphorylation was evaluated by Western blot analysis. The chymotrypsin-like activity of the proteasome was evaluated with a fluorogenic peptide, and intracellular calcium concentration was measured with fura-2. The results indicate that Ln stimulated the acrosome reaction of human sperm in a dose-dependent manner. This increase was drastically inhibited in the presence of herbimycin A, SU6656, and epoxomicin. In addition, Ln increased proteasome activity and phosphorylation; both events were inhibited by herbimycin A and SU6656. Finally, Ln induced an increase in intracellular calcium concentration, which was inhibited by SU6656 and epoxomicin. These results suggest that Ln is able to induce the acrosome reaction. This effect may be mediated by Src kinase and the proteasome, with the consequent induction of a calcium influx.

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Cited by 32 publications

References 51 publications

Assay and Importance of Adhesive Interaction Between Hamster (Mesocricetus auratus) Oocyte-Cumulus Complexes and the Oviductal Epithelium1

Assay and Importance of Adhesive Interaction Between Hamster (Mesocricetus auratus) Oocyte-Cumulus Complexes and the Oviductal Epithelium1

Cumulus Oophorus Extracellular Matrix: Its Construction and Regulation.

The Laminin-Induced Acrosome Reaction in Human Sperm Is Mediated by Src Kinases and the Proteasome1

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