Heterogeneous Response Patterns of Alveolar Macrophages from Patients with Lung Cancer by Stimulation with Interferon-gamma

Eifuku, Ryozo; Yoshimatsu, Takashi; Yoshino, Ichiro; Takenoyama, Mitsuhiro; Imahayashi, Satoru; Saito, Tomoko; Hanagiri, Takeshi; Nomoto, Kikuo; Yasumoto, Kosei

doi:10.1093/jjco/hyd077

Cited by 10 publications

(8 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Others have shown AM from patients with primary lung cancer suppressed chemotaxis and this intrinsic functional defect is more pronounced on AM from the local tumour site compared to the opposite side of the lung [3]. Eifuku et al [11] reported heterogeneous cytotoxic function of BAL AM in patients with lung cancer and suggested that the heterogeneity of response likely reflected variations in activation state of AM. Heterogeneity in cytotoxicity against the squamous cell lung cancer cell line QG56 was also noted in response to IFN‐γ stimulation in this study, where AM cytotoxic potential paralleled AM IL‐1 secretion.…”

Section: Discussionmentioning

confidence: 99%

“…Differences in up‐regulation of costimulatory molecules CD80 and CD86 have been observed in AM from patients with allergies [35] however, this has not been assessed in primary lung cancer patients. Phenotypic analysis suggests that there is no difference between CD68 and HLA‐DR expression in AM from lung cancer patients compared to controls however, a significant increase in CD11c, CD11b and CD11a was observed [11]. The ability of AM to stimulate T cells will include the phenotype of the cells.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Alveolar macrophage function is altered in patients with lung cancer

Pouniotis¹,

Plebanski²,

Apostolopoulos³

et al. 2005

Clinical and Experimental Immunology

View full text Add to dashboard Cite

SummaryThe alveolar macrophage (AM) is believed to be of central importance in the immune response against infection and tumour. We examined patients with lung cancer in order to evaluate the immuno-stimulatory potential of AM in lung cancer. Bronchoalveolar lavage fluid samples were obtained from patients with adenocarcinoma, squamous cell carcinoma, large cell undifferentiated lung carcinoma, small cell carcinoma and control subjects. AM were isolated and phagocytic function, flow cytometry and cytokine analysis were assessed. AM from patients with small and squamous cell carcinoma had impaired uptake in vitro of 40 nm fluorescent polystyrene beads. AM from patients with small, squamous and large cell undifferentiated carcinoma showed impaired uptake of 1000 nm fluorescent polystyrene beads. Secreted levels of TNF-α α α α and IL-1 from AM of patients with small, squamous, and large cell undifferentiated carcinoma were decreased compared to controls. Secreted AM IL-6 levels were decreased in small and large cell undifferentiated carcinoma. AM from adenocarcinoma patients showed similar levels of IL-10, IL-6, IL-1 and TNF-α α α α compared to controls. Phenotypic analysis demonstrated that patients with small cell carcinoma were the only group that showed a decrease in MHC class II surface expression. Surface expression of ICAM-1 and CD83 was decreased on AM from patients with large, squamous and small cell carcinoma compared to controls but not adenocarcinoma. Mannose receptor levels were only decreased on AM from patients with squamous and small cell carcinoma but not adenocarcinoma and large cell undifferentiated carcinoma. We conclude that there are type-specific alterations in uptake ability, cytokine secretion and phenotype of AM from lung cancer patients, which may result in an inability to stimulate anti-tumour immunity. The observed differences between lung cancer subgroups may explain previously reported inconsistencies in descriptions of AM characteristics in lung cancer.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Alveolar macrophage function is altered in patients with lung cancer

Pouniotis¹,

Plebanski²,

Apostolopoulos³

et al. 2005

Clinical and Experimental Immunology

View full text Add to dashboard Cite

show abstract

“…Increased TNF- α and IL-1 secretion from AMs of patients with lung cancer after stimulation with IFN- γ and granulocyte-macrophage colony-stimulating factor (GM-CSF) has been demonstrated in comparison with patients with nonmalignant disorders [ 7 ]. IL-1 mediates cytotoxicity and suppression of tumour growth [ 31 , 32 ] and TNF- α inhibits angiogenesis to prevent tumour growth. TNF- α can therefore act as an antitumour monokine and be responsible for spontaneous (without external activation) cytotoxic effects of AMs [ 33 ].…”

Section: Potential Role Of Alveolar Macrophages In Tumour Regressimentioning

confidence: 99%

“…Numerous studies have isolated AMs from bronchoalveolar lavage (BAL) for assessment of their function in the presence of known macrophage-activating agents such as IFN- γ and GM-CSF [ 8 , 13 , 15 , 31 , 34 ]. IFN- γ stimulates IFN- γ inducible protein (IP-10), an antitumour molecule that impairs tumour angiogenesis, whereas GM-CSF promotes proliferation of haematopoietic progenitor cells and influences the antitumour function of AMs [ 34 ].…”

Section: Potential Role Of Alveolar Macrophages In Tumour Regressimentioning

confidence: 99%

Alveolar Macrophage Polarisation in Lung Cancer

Almatroodi

McDonald

Pouniotis

2014

Lung Cancer International

View full text Add to dashboard Cite

The role of alveolar macrophages in lung cancer is multifaceted and conflicting. Alveolar macrophage secretion of proinflammatory cytokines has been found to enhance antitumour functions, cytostasis (inhibition of tumour growth), and cytotoxicity (macrophage-mediated killing). In contrast, protumour functions of alveolar macrophages in lung cancer have also been indicated. Inhibition of antitumour function via secretion of the anti-inflammatory cytokine IL-10 as well as reduced secretion of proinflammatory cytokines and reduction of mannose receptor expression on alveolar macrophages may contribute to lung cancer progression and metastasis. Alveolar macrophages have also been found to contribute to angiogenesis and tumour growth via the secretion of IL-8 and VEGF. This paper reviews the evidence for a dual role of alveolar macrophages in lung cancer progression.

show abstract

“…23). In vitro attempts to stimulate production of NO⅐ by human monocytes have failed, and the study of NO⅐ production by human AMs has been confined to systems in which these cells are already stimulated, such as idiopathic pulmonary fibrosis (36) and cancer (12).…”

Section: Killing Of Pathogens By Human and Murine Alveolar Macrophagementioning

confidence: 99%

Lung surfactant and reactive oxygen-nitrogen species: antimicrobial activity and host-pathogen interactions

Hickman-Davis

Fang

Nathan

et al. 2001

American Journal of Physiology-Lung Cellular and Molecular Phys

View full text Add to dashboard Cite

Surfactant protein (SP) A and SP-D are members of the collectin superfamily. They are widely distributed within the lung, are capable of antigen recognition, and can discern self versus nonself. SPs recognize bacteria, fungi, and viruses by binding mannose and N-acetylglucosamine residues on microbial cell walls. SP-A has been shown to stimulate the respiratory burst as well as nitric oxide synthase expression by alveolar macrophages. Although nitric oxide (NO.) is a well-recognized microbicidal product of macrophages, the mechanism(s) by which NO. contributes to host defense remains undefined. The purpose of this symposium was to present current research pertaining to the specific role of SPs and reactive oxygen-nitrogen species in innate immunity. The symposium focused on the mechanisms of NO*-mediated toxicity for bacterial, human, and animal models of SP-A- and NO.-mediated pathogen killing, microbial defense mechanisms against reactive oxygen-nitrogen species, specific examples and signaling pathways involved in the SP-A-mediated killing of pulmonary pathogens, the structure and binding of SP-A and SP-D to bacterial targets, and the immunoregulatory functions of SP-A.

show abstract

Heterogeneous Response Patterns of Alveolar Macrophages from Patients with Lung Cancer by Stimulation with Interferon-gamma

Abstract: The AM have anti-tumor cytotoxicity in lung cancer although the cytolytic potential is heterogeneous and that the tumor lysis by AM is mediated by both TNF-alpha and NO production.

Cited by 10 publications

References 20 publications

Alveolar macrophage function is altered in patients with lung cancer

Alveolar macrophage function is altered in patients with lung cancer

Alveolar Macrophage Polarisation in Lung Cancer

Lung surfactant and reactive oxygen-nitrogen species: antimicrobial activity and host-pathogen interactions

Contact Info

Product

Resources

About