Heterogeneous mitochondrial DNA D-loop sequences in bovine tissue

Hauswirth, William W.; Walle, M J Van de; Olivo, Paul D.; Laipis, Philip J.

doi:10.1016/0092-8674(84)90434-3

Cited by 122 publications

(70 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These may result from strand slippage and mismatching during replication (Densmore et al, 1985); in the absence of sequence data, it is not possible to say whether or not there are shared end points. The fuzzy bands observed in some Treinja and Guberevac samples imply that these newts were heteroplasmic for a continuous distribution of small length variations of 1-15 bp, as found in the conserved sequence blocks (CSBs) of mammals (Hauswirth et a!., 1984;Hauswirth and Clayton, 1985). This parallels findings in Rana in which fuzziness over a 400-700 bp range has been described .…”

Section: Discussionsupporting

confidence: 69%

“…1 and and Greenberg et aL (1983) describe 5 small length variations amounting to nearly 9 per cent of the total variation observed. Brown and DesRosiers (1983) describe length variation in homopolymer runs of up to 4 bp between tRNACYS and tRNATYr near the origin of L-strand replication in Rattus, and similar findings are reported in the D-loops of cows (Hauswirth et aL, 1984) and humans (Hauswirth and Clayton, 1985). A study of 208 individuals representing 8 species of Mus revelaed only one insertion variant: 12 bp (in or near the D-loop) in a single mouse (Ferris et a!., 1983).…”

Section: Discussionmentioning

confidence: 65%

“…The CSBs can be drawn into large hairpin structures, and may be related to insertion polymorphism in the region. Repeat sequences of 45 bp separated by 16 bp have Solignac et aL, 1983Hauswirth et aL, 1984Monnerot eta!., 1984Harrison et aL, 1985Densmore et aL, 1985Bermingham et aL, 1986 this paper * 30th generation of isofemale line.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Mitochondrial DNA insertion polymorphism and germ line heteroplasmy in the Triturus cristatus complex

Wallis

1987

Heredity

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 69%

Section: Discussionmentioning

confidence: 65%

See 1 more Smart Citation

Mitochondrial DNA insertion polymorphism and germ line heteroplasmy in the Triturus cristatus complex

Wallis

1987

Heredity

View full text Add to dashboard Cite

“…Only a few cases of mitochondria1 heteroplasmy, not related to any disease, have been described in mammals (in cow, Hauswirth and Laipis, 1982;in man, Hauswirth and Clayton, 1985). In both reports the molecules differ in length by one or a few nucleotides.…”

Section: Molecular Mechanisms Generating Mitochondria1 Heteroplasmymentioning

confidence: 99%

Direct repeats in the non‐coding region of rabbit mitochondrial DNA

Mignotte

Guéride

Champagne

et al. 1990

European Journal of Biochemistry

View full text Add to dashboard Cite

In rabbit we observed heteroplasmy at an exceptionally high level, the heterogeneity occurring within the noncoding region of the DNA. Mitochondria1 DNA (mt DNA) was cloned in pBR322 and the nucleotide sequence analysis of an EcoRI -Hind I11 fragment encompassing the non-coding region revealed that although there are common features with other mammalian mtDNAs (termed large central-conserved-sequence block, conservedsequence blocks 1, 2 and 3 and termination-associated elements) the non-coding region shows an unusual organization; two stretches of tandem repeats of 20 bp and 1.53 bp are present in a part containing the origin of H-strand replication (Of,) and probably the promoters for transcription as judged from other vertebrates. The long repeats are located between tRNAPhc and conserved sequence block 3 and the short repeats are located between conserved sequence blocks 1 and 2. When cloned in Escherichiu coli (recA or recBC sbcb) DNA fragments containing the short repeats show length differences corresponding to various copy numbers of repeats. Electrophoretic analysis of the appropriate restriction fragments of rabbit mtDNA reveals extended intra-and inter-individual length heterogeneity. Both sets of repeats are involved in the generation of heterogeneity and are present in variable copy numbers from one mtDNA molecule to another. Moreover, rearrangement of the motives of the short repeat are observed to different extents in the mtDNA from one animal to another. The occurrence, maintenance and possible involvement of these repeated sequences, capable of forming stable secondary structures, are discussed in relation to their location in the region of control signals.Mammalian mitochondrial DNAs (mtDNAs) range in size from 16 kb to 17.5 kb. The complete nucleotide sequences of human (Andersonet al., 1981)mouse (Bibbet al., 1981) bovine (Anderson et al., 1982) and rat (Gadaleta et al., 1989) mtDNAs reveal an overall conservation of gene order and very compact organization of genetic information (for reviews see Attardi, 1985, and Tzagoloff, 1985). In all species the noncoding sequences consist of a few short intergenic segments and a large non-coding region located between the genes coding for tRNAPhe and tRNAPr0.The length of this non-coding region varies among species and only the central part exhibits extended nucleotide sequence similarities. This large central-conserved sequence block appears to be one of the mtDNA's most conserved regions, other parts of the non-coding region are the least conserved sequences of the molecule between species. At the level of the species the large conserved sequence block does not diverge anymore than the mitochondrial genes coding for polypeptides (Brown et al., 1986). Several authors have shown that despite sequence diversity these regions share similar physical properties (Mignotte et al., 1987) and potential secondary structures (Brown et al., 1986 andBrun, 1987). The evolutionary pressure on the non-coding region seems to take place at the level of the secondary ...

show abstract

“…The genetic characteristics of these length polymorphisms have been the subject of investigations to gain insights into the factors that influence the transmission of the mtDNA, in particular in relation to the germ-line bottleneck (Hauswirth et al 1984;Marchington et al 1997). One of the homopolymeric tracts, which is associated with a mtDNA T16189C variant located within and interrupting a homopolymeric tract of cytosines between nt16184 and nt16193 (Bendall and Sykes 1995;Marchington et al 1996), shows a prominent deviation from the common rules that govern mtDNA transmission, and is thus of great interest.…”

Section: Introductionmentioning

confidence: 99%

Evidence for the de novo regeneration of the pattern of the length heteroplasmy associated with the T16189C variant in the control (D-loop) region of mitochondrial DNA

et al. 2002

View full text Add to dashboard Cite

We have investigated the genetics of the length heteroplasmy associated with the T16189C variant of mitochondrial DNA (mtDNA), and report here definitive evidence that the pattern of the length heteroplasmy is not simply the outcome of drift related to the random segregation of the mtDNA population during cell division, but is actively maintained and regenerated de novo following each cell division. The pattern of the length heteroplasmy was maintained in a fibroblast cell line during an extensive mtDNA depletion experiment by ethidium bromide treatment, and following the subsequent repopulation of the cells with mtDNA. The investigation of the pattern of the length heteroplasmy by single cell pick up shows a similar pattern in sister cells despite the evidence of the randomness of mtDNA segregation, providing definitive evidence for the de novo regeneration of the pattern following cell division. Consistent with this conclusion is the observation that similar patterns of length heteroplasmy are found in tissues of single individuals.

show abstract

Heterogeneous mitochondrial DNA D-loop sequences in bovine tissue

Cited by 122 publications

References 28 publications

Mitochondrial DNA insertion polymorphism and germ line heteroplasmy in the Triturus cristatus complex

Mitochondrial DNA insertion polymorphism and germ line heteroplasmy in the Triturus cristatus complex

Direct repeats in the non‐coding region of rabbit mitochondrial DNA

Evidence for the de novo regeneration of the pattern of the length heteroplasmy associated with the T16189C variant in the control (D-loop) region of mitochondrial DNA

Contact Info

Product

Resources

About