Heterogeneous micromechanical properties of the extracellular matrix in healthy and infarcted hearts

Andreu, Ion; Luque, Tomás; Sancho, Ana; Pelacho, Beatriz; Iglesias-García, Olalla; Melo, Esther; Farré, Ramón; Prósper, Felipe; Elizalde, M.R.; Navajas, Daniel

doi:10.1016/j.actbio.2014.03.034

Cited by 48 publications

(33 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In support of this interpretation, a computational model of pulmonary mechanics predicted that the elasticity of the alveolar walls were one order of magnitude higher than that of tissue strips (Cavalcante et al, 2005). In addition to lung tissue sections, we have examined the elasticity of 12-lm-thick heart tissue samples from wild-type and infarcted mice, and found E $30 kPa in collagen-rich regions within the myocardium, which increased $three-fold in infarcted heart concomitantly with a dramatic increase in collagen deposition (Andreu et al, 2014), which is in qualitative agreement with our observations on acellular collagen-I gels (Alcaraz et al, 2011). Altogether these observations reveal intrinsic differences in the elasticity of acellular and whole tissue sections, which should be taken into consideration when designing mechanobiology studies based on hydrogels with tunable elasticity.…”

Section: Limitations Of 2d Paa Gels With Tunable Elasticity For Mecmentioning

confidence: 99%

“…Finally, detergents or other chemical agents are used to wash cell debris resulting from the previous procedures (Jorba et al, ). Although each tissue requires optimization of the decellularization protocol, we and other groups have already reported useful protocols to remove all cell components while preserving ECM structure and compositon for a variety of mammalian tissues and organs including lung and heart (Andreu et al, ; Luque et al, ).…”

Section: Elastic Properties Of Decellularized Tissue Sectionsmentioning

confidence: 99%

See 1 more Smart Citation

Elastic properties of hydrogels and decellularized tissue sections used in mechanobiology studies probed by atomic force microscopy

Giménez

Uriarte

Vieyra

et al. 2016

Microscopy Res & Technique

Self Cite

View full text Add to dashboard Cite

The increasing recognition that tissue elasticity is an important regulator of cell behavior in normal and pathologic conditions such as fibrosis and cancer has driven the development of cell culture substrata with tunable elasticity. Such development has urged the need to quantify the elastic properties of these cell culture substrata particularly at the nanometer scale, since this is the relevant length scale involved in cell-extracellular matrix (ECM) mechanical interactions. To address this need, we have exploited the versatility of atomic force microscopy to quantify the elastic properties of a variety of cell culture substrata used in mechanobiology studies, including floating collagen gels, ECM-coated polyacrylamide gels, and decellularized tissue sections. In this review we summarize major findings in this field from our group within the context of the state-of-the-art in the field, and provide a critical discussion on the applicability and complementarity of currently available cell culture assays with tunable elasticity. In addition, we briefly describe how the limitations of these assays provide opportunities for future research, which is expected to continue expanding our understanding of the mechanobiological aspects that support both normal and diseased conditions. Microsc. Res. Tech. 80:85-96, 2017. © 2016 Wiley Periodicals, Inc.

show abstract

Section: Limitations Of 2d Paa Gels With Tunable Elasticity For Mecmentioning

confidence: 99%

Section: Elastic Properties Of Decellularized Tissue Sectionsmentioning

confidence: 99%

Elastic properties of hydrogels and decellularized tissue sections used in mechanobiology studies probed by atomic force microscopy

Giménez

Uriarte

Vieyra

et al. 2016

Microscopy Res & Technique

Self Cite

View full text Add to dashboard Cite

show abstract

“…Combination of closed- and open-chest measurements allowed us to separately quantify lung and chest wall elastances. Second, we measured the local stiffness of the ECM by AFM on decellularized lungs, a technique that has been recently employed in a variety of settings to assess the micromechanics of tissues and organ scaffolds [ 35 – 37 ]. Although measuring the local stiffness of lung ECM by AFM involved sample processing (decellularization and slice sectioning) that could slightly affect the results [ 24 ], the technique employed in this work preserves the architecture of the lung scaffold ( S1 Fig ) and was previously able to detect a wide range of lung ECM stiffness changes caused by ageing and fibrosis [ 31 ].…”

Section: Discussionmentioning

confidence: 99%

Early Impairment of Lung Mechanics in a Murine Model of Marfan Syndrome

et al. 2016

Self Cite

View full text Add to dashboard Cite

Early morbidity and mortality in patients with Marfan syndrome (MFS) -a connective tissue disease caused by mutations in fibrillin-1 gene- are mainly caused by aorta aneurysm and rupture. However, the increase in the life expectancy of MFS patients recently achieved by reparatory surgery promotes clinical manifestations in other organs. Although some studies have reported respiratory alterations in MFS, our knowledge of how this connective tissue disease modifies lung mechanics is scarce. Hence, we assessed whether the stiffness of the whole lung and of its extracellular matrix (ECM) is affected in a well-characterized MFS mouse model (FBN1C1039G/+). The stiffness of the whole lung and of its ECM were measured by conventional mechanical ventilation and atomic force microscopy, respectively. We studied 5-week and 9-month old mice, whose ages are representative of early and late stages of the disease. At both ages, the lungs of MFS mice were significantly more compliant than in wild type (WT) mice. By contrast, no significant differences were found in local lung ECM stiffness. Moreover, histopathological lung evaluation showed a clear emphysematous-like pattern in MFS mice since alveolar space enlargement was significantly increased compared with WT mice. These data suggest that the mechanism explaining the increased lung compliance in MFS is not a direct consequence of reduced ECM stiffness, but an emphysema-like alteration in the 3D structural organization of the lung. Since lung alterations in MFS are almost fully manifested at an early age, it is suggested that respiratory monitoring could provide early biomarkers for diagnosis and/or follow-up of patients with the Marfan syndrome.

show abstract

“…[ 37,38 ] AFM stiffness mapping studies have found heterogeneity in heart stiffness, as seen in other tissues, and studies of fibrotic hearts show that damaged and scarred areas have higher moduli than healthy regions. [ 39 ]…”

Section: Mechanical Changes and Pathogenesis Of Fibrosis Across Tissuesmentioning

confidence: 99%

Engineered Biomaterial Platforms to Study Fibrosis

Davidson

Burdick

Wells

2020

Adv Healthcare Materials

View full text Add to dashboard Cite

Many pathologic conditions lead to the development of tissue scarring and fibrosis, which are characterized by the accumulation of abnormal extracellular matrix (ECM) and changes in tissue mechanical properties. Cells within fibrotic tissues are exposed to dynamic microenvironments that may promote or prolong fibrosis, which makes it difficult to treat. Biomaterials have proved indispensable to better understand how cells sense their extracellular environment and are now being employed to study fibrosis in many tissues. As mechanical testing of tissues becomes more routine and biomaterial tools become more advanced, the impact of biophysical factors in fibrosis are beginning to be understood. Herein, fibrosis from a materials perspective is reviewed, including the role and mechanical properties of ECM components, the spatiotemporal mechanical changes that occur during fibrosis, current biomaterial systems to study fibrosis, and emerging biomaterial systems and tools that can further the understanding of fibrosis initiation and progression. This review concludes by highlighting considerations in promoting wide‐spread use of biomaterials for fibrosis investigations and by suggesting future in vivo studies that it is hoped will inspire the development of even more advanced biomaterial systems.

show abstract

Heterogeneous micromechanical properties of the extracellular matrix in healthy and infarcted hearts

Cited by 48 publications

References 33 publications

Elastic properties of hydrogels and decellularized tissue sections used in mechanobiology studies probed by atomic force microscopy

Elastic properties of hydrogels and decellularized tissue sections used in mechanobiology studies probed by atomic force microscopy

Early Impairment of Lung Mechanics in a Murine Model of Marfan Syndrome

Engineered Biomaterial Platforms to Study Fibrosis

Contact Info

Product

Resources

About