Heterogeneous expression of the aquaporin 1 (AQP1) water channel in tumors of the prostate, breast, ovary, colon and lung: A study using high density multiple human tumor tissue microarrays

Mobasheri, Ali; Airley, Rachel; Hewitt, Stephen M.; Marples, David

doi:10.3892/ijo.26.5.1149

Cited by 64 publications

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“…Aquaporin 3 was found to be expressed in normal and malignant prostate tissue and may be involved in tumour initiation and development (Wang et al, 2007). The expression of AQP1 is confined to the capillary endothelium of prostate cancer cells and it may be involved in microvascular alteration during tumour angiogenesis (Mobasheri et al, 2005). There has been increasing evidence that AQPs may play a role in carcinogenesis.…”

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confidence: 99%

Inhibition of the aquaporin 3 water channel increases the sensitivity of prostate cancer cells to cryotherapy

et al. 2009

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Aquaporins (AQPs) are intrinsic membrane proteins that facilitate selective water and small solute movement across the plasma membrane. In this study, we investigate the role of inhibiting AQPs in sensitising prostate cancer cells to cryotherapy. PC-3 and DU145 prostate cancer cells were cooled to 0, À5 and À101C. The expression of AQP3 in response to freezing was determined using realtime quantitative polymerase chain reaction (RT -qPCR) and western blot analysis. Aquaporins were inhibited using mercuric chloride (HgCl 2 ) and small interfering RNA (siRNA) duplex, and cell survival was assessed using a colorimetric assay. There was a significant increase in AQP3 expression in response to freezing. Cells treated with AQP3 siRNA were more sensitive to cryoinjury compared with control cells (Po0.001). Inhibition of the AQPs by HgCl 2 also increased the sensitivity of both cell lines to cryoinjury and there was a complete loss of cell viability at À101C (Po0.01). In conclusion, we have shown that AQP3 is involved directly in cryoinjury. Inhibition of AQP3 increases the sensitivity of prostate cancer cells to freezing. This strategy may be exploited in the clinic to improve the efficacy of prostate cryotherapy.

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confidence: 99%

Inhibition of the aquaporin 3 water channel increases the sensitivity of prostate cancer cells to cryotherapy

et al. 2009

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“…We previously reported that AQP-5 may be involved in differentiation of human gastric carcinoma cells, and overexpression of several aquaporins has been reported in different types of human carcinoma (8)(9)(10)(11)(12)(13)(14)(15)(16). However, the role of AQPs in biliary tract carcinogenesis has not yet been clearly defined.…”

Section: Introductionmentioning

confidence: 99%

Prognostic significance of aquaporins in human biliary tract carcinoma

et al. 2012

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Abstract. Aquaporins (AQPs) are important in controlling bile formation, however, the exact role of AQPs in human biliary tract carcinogenesis has not been clearly defined. In this study, we analyzed AQP-1, -4, -5 and -8 expression immunohistochemically using tissue microarrays (TMAs) in 81 samples. (45 gallbladder carcinomas and 36 bile duct carcinomas). The survival of patients with high AQP-5 expression was longer compared to that of patients with low AQP-5 expression (P=0.017). Cox's proportional hazard model revealed that AQP-5 expression was an independent prognostic factor (RR, 0.38; P=0.025). Chi-square analysis revealed that high AQP-5 expression correlated to small tumor size in biliary tract carcinoma patients (P=0.006). With regard to the expression of other AQPs, depth of tumor invasion, histological type and serum carbohydrate antigen 19-9 (CA19-9) were associated with high AQP-1 expression (P=0.039, 0.011 and 0.032). However, AQP-4 and AQP-8 expression had no association with clinicopathological factors. Among the 10 patients who underwent gemcitabine (GEM) plus S-1 postoperative chemotherapy, the group of patients (n=5) with high AQP-5 expression were associated with higher rates of both overall and disease-free survival (log-rank P=0.033, 0.002). In conclusion, the results of this study suggest that AQP-5 expression may be associated with prognosis and drug sensitivity in biliary tract carcinoma.

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“…However, concerning AQPs in breast cancer, the published data are limited. AQP1 could not be detected in a series of liver metastases of metastatic breast cancer patients (15), and Mobasheri et al demonstrated that the expression of AQP1 was differentially altered in tumors of the breast (16), showing that AQP1 expression was upregulated in the microvessel endothelia and neoplastic cells of selected ductal adenocarcinomas. This was in agreement with data previously published by Endo et al (36).…”

Section: Discussionmentioning

confidence: 99%

“…To date, eleven mammalian AQPs have been identified. These are distributed ubiquitously throughout the human body, and are believed to play a role in hypertension and heart failure (12,13) and, moreover, in oncology, carcinogenesis and angiogenesis (14)(15)(16)(17)(18)(19)(20)(21)(22).…”

Section: Introductionmentioning

confidence: 99%

Prognostic relevance of the AQP5 −1364C>A polymorphism in primary breast cancer

Kasimir‐Bauer

Heubner²,

Otterbach³

et al. 2009

Mol Med Rep

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Abstract. Besides known prognostic factors in breast cancer, disseminated tumor cells are regarded as a surrogate marker for minimal residual disease in breast cancer. Their prognostic significance is comparable to that of lymph node status. However, the mechanism by which the primary tumor directs cells to disseminate into lymph nodes or into blood vessels is unclear. Aquaporins (AQPs) are small integral membrane proteins that provide a major pathway for water transport throughout several organs, and have been shown to be of potential importance in various types of cancer. Here, we analyzed the single nucleotide polymorphism (SNP) A(-1364)C in AQP5 in 107 patients with early stage breast cancer in order to test the hypothesis that this polymorphism is associated with lymphogenous and hematogenous tumor cell dissemination and overall survival. Paraffin-embedded tumor tissue was dewaxed, and DNA was extracted from tumor tissues using the QIAamp Blood DNA Mini Kit. The quantification and quality of the extracted DNA were determined spectrophotometrically. Genotyping of the -1364A>C polymorphism was performed by Pyrosequencing. Cytokeratinpositive (CK + ) bone marrow (BM) cells were isolated by density gradient centrifugation followed by immunocytochemistry, applying the pan CK antibody A45-B/B3. There was no evidence of an association between the AQP5C>A genotypes and an increased risk of developing breast cancer, nor between the genotypes and tumor size, lymph node involvement, distant metastasis, grade, histopathology, expression of estrogen receptor and HER2, or the dissemination of tumor cells to BM. In contrast, when comparing C-allele carriers with patients carrying the AA genotype, expression of the progesterone receptor differed significantly between these genotype groups (mean AA genotype 51%, mean AC and CC genotype 73%; p=0.039). Additionally, a significant correlation was found between progesterone receptor expression and adjuvant chemotherapy (p=0.021) and adjuvant endocrine therapy (p=0.017), respectively. SNPs in AQP5 are not associated with hematogenous or lymphogenous tumor cell spread. However, we observed for the first time an association between SNPs in AQP5 and progesterone receptor positivity, which might have implications for future adjuvant treatment. Further investigations must include more than one AQP as well as factors promoting angiogenesis to elucidate the different modes of tumor cell dissemination.

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Heterogeneous expression of the aquaporin 1 (AQP1) water channel in tumors of the prostate, breast, ovary, colon and lung: A study using high density multiple human tumor tissue microarrays

Cited by 64 publications

References 0 publications

Inhibition of the aquaporin 3 water channel increases the sensitivity of prostate cancer cells to cryotherapy

Inhibition of the aquaporin 3 water channel increases the sensitivity of prostate cancer cells to cryotherapy

Prognostic significance of aquaporins in human biliary tract carcinoma

Prognostic relevance of the AQP5 −1364C>A polymorphism in primary breast cancer

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