Heterogeneous Expression of Myelo-Monocytic Markers on Selected Non-Lymphoid Cells

Cattoretti, Giorgio; Delia, Domenico; Parola, Luciana; Schirò, R; Valeggio, C.; Simoni, Giuseppe; Romitti, Lorenza; Polli, N; Deliliers, Giorgio Lambertenghi; Ferrari, Maurizio; Cantù-Rajnoldi, A

doi:10.1007/978-1-4612-4850-7_18

Cited by 2 publications

(1 citation statement)

References 13 publications

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“…We have found that sheep afferent dendritic cells, like human and mouse Langerhans' cells, express CDl. This family of molecules is best described in the human system where three subgroups are defined, CDla, CDlb, and CDlc, that have glycosylated heavy chains of 49, 45, and 43 kD, respectively, and that can be differentiated on the basis of their tissue distribution (38) . All the anti-human CDl mAbs stain cortical thymocytes, but while anti-CDla monoclonals stain both dermal and epidermal dendritic cells, CDlb monoclonals stain only dermal dendritic cells.…”

Section: Discussionmentioning

confidence: 99%

Characterization of sheep afferent lymph dendritic cells and their role in antigen carriage.

Bujdoso

Hopkins

Dutia

et al. 1989

The Journal of Experimental Medicine

183

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We have ablated peripheral lymph nodes in sheep and subsequently cannulated the pseudo-afferent lymphatic vessel that arises as a consequence of afferent lymphatic vessels reanastomosing with the former efferent duct. This technique allows the collection of lymph with a cellular composition that resembles true afferent fluid, and in particular, containing 1-10% dendritic cells. A 16-h collection of this lymph may contain between 10(6) and 10(7) dendritic cells. This dendritic cell population may be enriched to greater than 75% by a single-density gradient centrifugation step. We have generated a mAb that recognizes sheep CD1. This monoclonal not only reacts with afferent dendritic cells, but with dendritic cells in the skin and paracortical T cell areas of lymph nodes. The expression of CD1 suggests afferent dendritic cells are related to skin Langerhans' cells and other dendritic cells that act as accessory cells for T cell responses. Consistent with this is the high level of expression by dendritic cells of molecules involved in antigen recognition by T cells, including MHC class I and class II. Afferent dendritic cells express high levels of the cellular adhesion molecule LFA-3, and at the same time express a ligand for this molecule, namely CD2. The accessory functions of afferent dendritic cells resemble those displayed by mature Langerhans' cells and by lymph node interdigitating cells. These include clustering with resting T cells and stimulating their proliferation in a primary response to antigen. Afferent dendritic cells are capable of acquiring soluble protein antigen in vivo or in vitro and presenting the material directly to autologous T cells in an antigen-specific manner. We conclude that afferent dendritic cells represent a lymph-borne Langerhans' cell involved in antigen carriage to the lymph node.

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Section: Discussionmentioning

confidence: 99%

Characterization of sheep afferent lymph dendritic cells and their role in antigen carriage.

Bujdoso

Hopkins

Dutia

et al. 1989

The Journal of Experimental Medicine

183

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Neuroblastoma during acute lymphoblastic leukemia in remission

Uderzo

Rajnoldi

Schirò

et al. 1988

Cancer

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Neuroblastoma was diagnosed in a child after a 20-month remission of a pre-B acute lymphoblastic leukemia (ALL). Clumps of atypical cells suggestive of neuroblastoma were seen in the bone marrow. They were positive for monoclonal antibody (MoAb) UJ13A (neuroblastoma cells) and negative for MoAb T29/33 (anti-leucocyte common antigen CD45) with immunocytochemical staining. A right paravertebral mass displacing the kidney was demonstrated by abdominal echotomography, and serum vanilmandelic acid was slightly increased. Despite specific chemotherapy against neuroblastoma and after a transient clinical improvement, the patient died 7 months later of disseminated disease. Immunocytochemical staining on cells frozen at diagnosis of leukemia with MoAb UJ13A and T29/33 was unable to demonstrate neuroblastoma cells and showed the pattern usually observed in leukemia (UJ13A- and T29/33+).

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Heterogeneous Expression of Myelo-Monocytic Markers on Selected Non-Lymphoid Cells

Cited by 2 publications

References 13 publications

Characterization of sheep afferent lymph dendritic cells and their role in antigen carriage.

Characterization of sheep afferent lymph dendritic cells and their role in antigen carriage.

Neuroblastoma during acute lymphoblastic leukemia in remission

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