Heterogeneous distribution of a fatty acid analogue uptake in the myocardium of aged rats

Abstract

The aim of this study was to determine the extent and location of damaged myocardial areas in senescent rats. The viability of myocardial cells was evaluated in virgin young (4 months old) and aged (29 months old) female Wistar rats by analysing the uptake of a slowly metabolisable radiolabelled fatty acid analogue, 15-p-iodophenyl-beta-methylpentadecanoic acid (IMPPA). The biodistribution of IMPPA was measured in various organs, and regional myocardial uptake was specifically assessed using quantitative autoradiography. Myocardial enzymatic activity and DNA content were also evaluated with nitro blue tetrazolium (NBT) and propidium iodide (PI) staining, respectively. In senescent rats, cardiac and renal IMPPA uptake showed a significant (50%) reduction compared with young adult rats and the uptake was not significantly changed in the liver, spleen, lungs, and skeletal muscle. Total ventricular NBT staining and IMPPA uptake were almost homogeneous in young adult rats, whereas they were very heterogeneous in aged rats. In the latter, approximately 11% of the total ventricular volume showed a significantly decreased (by 60% or more) IMPPA uptake compared with normal values, and this reduction was greater in ventricle base than in apex. The myocardial areas unlabelled or poorly labelled by IMPPA represented 4, 5, 6, and 21% of the right ventricular, left ventricular epicardial, septal, and left ventricular endocardial volume, respectively, and were poorly stained with NBT. In some of these areas, PI staining indicated the presence of living cells unable to pick up NBT staining. In conclusion, in young adult rats, no myocardial lesions were observed using three different labelling techniques.(ABSTRACT TRUNCATED AT 250 WORDS)

Decreased Efficiency of Adenovirus-Mediated Gene Transfer in Aging Cardiomyocytes

“…These alterations lead to a defect in vascular supply, associated with multiple foci of replacement fibrosis (and changes in extracellular matrix), local ischemia, and decreased metabolism, as well as necrosis and apoptosis of myocytes. 4,5 The change in extracellular matrix surrounding mature skeletal muscle cells has especially been noted to impair adenoviral transduction efficiency. 17 However, it is not known whether the decreased infectivity observed in vivo is due to a decrease in infectivity at the level of the aged cardiomyocyte.…”