Heterogeneous chemistry in the 3-D state: an original approach to generate bioactive, mechanically-competent bone scaffolds

Tampieri, Anna; Ruffini, Andrea; Ballardini, Alberto; Montesi, Monica; Panseri, Silvia; Salamanna, Francesca; Fini, Milena; Sprio, Simone

doi:10.1039/c8bm01145a

Cited by 30 publications

(57 citation statements)

References 53 publications

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“…This effect can be ascribed to a reduction of the crystalline domain size because of the presence of doping Mg 2+ and Zn 2+ ions as substitutes for Ca 2+ and of CO 3 2− ions for PO 4 3− , as also observed in previous works. 10,14,20,28,29 FTIR spectra (Figure 1b) confirm the presence of the characteristic absorption bands of the phosphate group at 980−1100 and 560−600 cm −1 typical of the HA phase. The FTIR adsorption bands appear weakly resolved, typical for lowcrystalline materials, as confirmed by the splitting factors reported in Table 1.…”

Section: Resultsmentioning

confidence: 61%

Surface Phenomena Enhancing the Antibacterial and Osteogenic Ability of Nanocrystalline Hydroxyapatite, Activated by Multiple-Ion Doping

Sprio

Preti

Montesi

et al. 2019

ACS Biomater. Sci. Eng.

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The present work describes a novel nanocrystalline, multidoped hydroxyapatite featuring excellent eukaryotic versus prokaryotic cell selectivity, attested by excellent osteoinductive character and evaluated with human stem cells, and anti-infective ability, tested against different pathogens. Physicochemical analysis and transmission electron microscopy (TEM)/scanning STEM observations highlighted that such enhanced biological features are related to the lower crystallinity level and increased surface charge of hydroxyapatite, both induced by multiple-ion doping. Specifically, the lattice substitution of Ca 2+ with Zn 2+ promotes the segregation of Ca 2+ and doping Mg 2+ cations to a less-ordered surface layer, thus promoting dynamic ion absorption/release acting as bioactive signals for cells and exerting an antiproliferative effect on all tested pathogens. These findings open the design of new biodevices, combining regenerative ability and effective microbial inhibition without using any antibiotic drugs. This is extremely important to circumvent bacterial resistance to antibiotics, which is today considered as one of the biggest threats to global health.

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Section: Resultsmentioning

confidence: 61%

Surface Phenomena Enhancing the Antibacterial and Osteogenic Ability of Nanocrystalline Hydroxyapatite, Activated by Multiple-Ion Doping

Sprio

Preti

Montesi

et al. 2019

ACS Biomater. Sci. Eng.

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“…4). The HA scaffold is a sintered porous ceramic body obtained by a direct foaming process yielding open and highly interconnected macro/micro-porosity, thanks to the controlled formation of air bubbles incorporated into the hydroxyapatite ceramic slurry, obtained as the precursor of the final device 28,34,42,43,[47][48][49][50][51] . Both the biomimetic scaffolds show a nanostructural organisation that, along with their peculiar chemistry, enhances the cell adhesion, migration and subsequent cell colonisation.…”

Section: Discussionmentioning

confidence: 99%

Scaffold-based 3D cellular models mimicking the heterogeneity of osteosarcoma stem cell niche

Bassi

Panseri

Dozio

et al. 2020

Sci Rep

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The failure of the osteosarcoma conventional therapies leads to the growing need for novel therapeutic strategies. The lack of specificity for the Cancer Stem Cells (CSCs) population has been recently identified as the main limitation in the current therapies. Moreover, the traditional two-dimensional (2D) in vitro models, employed in the drug testing and screening as well as in the study of cell and molecular biology, are affected by a poor in vitro-in vivo translation ability. To overcome these limitations, this work provides two tumour engineering approaches as new tools to address osteosarcoma and improve therapy outcomes. In detail, two different hydroxyapatite-based bone-mimicking scaffolds were used to recapitulate aspects of the in vivo tumour microenvironment, focusing on CSCs niche. The biological performance of human osteosarcoma cell lines (MG63 and SAOS-2) and enriched-CSCs were deeply analysed in these complex cell culture models. The results highlight the fundamental role of the tumour microenvironment proving the mimicry of osteosarcoma stem cell niche by the use of CSCs together with the biomimetic scaffolds, compared to conventional 2D culture systems. These advanced 3D cell culture in vitro tumour models could improve the predictivity of preclinical studies and strongly enhance the clinical translation.

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“…While there is much potential for wood-based scaffolds and biomorphic transformation, certain drawbacks exist in comparison with other approaches. For instance, the process of biomorphic synthesis requires complex and strict control of reaction kinetics to avoid deformations and structural defects and to maintain the multiscale porosity (i.e., down to the nanoporosity) (Tampieri et al, 2018). Further, it relies on gassolid reactions that are strongly affected by different phenomena relating to adsorption of the gaseous reactant by the solid, kinetics of nucleation and growth of synthesized inorganic phase at the surface, and the penetration of the gaseous reactant within the innermost portion of the structure (Szekely, 2012).…”

Section: Scaffolds Synthesized Through Biomorphic Transformationmentioning

confidence: 99%

Nanostructured Biomaterials for Bone Regeneration

Lyons

Plantz

Hsu

et al. 2020

Front. Bioeng. Biotechnol.

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This review article addresses the various aspects of nano-biomaterials used in or being pursued for the purpose of promoting bone regeneration. In the last decade, significant growth in the fields of polymer sciences, nanotechnology, and biotechnology has resulted in the development of new nano-biomaterials. These are extensively explored as drug delivery carriers and as implantable devices. At the interface of nanomaterials and biological systems, the organic and synthetic worlds have merged over the past two decades, forming a new scientific field incorporating nano-material design for biological applications. For this field to evolve, there is a need to understand the dynamic forces and molecular components that shape these interactions and influence function, while also considering safety. While there is still much to learn about the bio-physicochemical interactions at the interface, we are at a point where pockets of accumulated knowledge can provide a conceptual framework to guide further exploration and inform future product development. This review is intended as a resource for academics, scientists, and physicians working in the field of orthopedics and bone repair.

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Heterogeneous chemistry in the 3-D state: an original approach to generate bioactive, mechanically-competent bone scaffolds

Abstract: Heterogeneous gas–solid reactions drive the biomorphic transformation of natural wood into large 3-D osteoinductive hydroxyapatite scaffolds with damage-tolerant mechanical performance.

Cited by 30 publications

References 53 publications

Surface Phenomena Enhancing the Antibacterial and Osteogenic Ability of Nanocrystalline Hydroxyapatite, Activated by Multiple-Ion Doping

Surface Phenomena Enhancing the Antibacterial and Osteogenic Ability of Nanocrystalline Hydroxyapatite, Activated by Multiple-Ion Doping

Scaffold-based 3D cellular models mimicking the heterogeneity of osteosarcoma stem cell niche

Nanostructured Biomaterials for Bone Regeneration

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