Heterogeneous Alterations in Human Alloimmunity Associated with Immunization

Roddy, Meagan; Clemente, Michael J.; Poggio, Emilio D.; Bukowski, Ronald; Thakkar, Snehal Govind; Waxenecker, Günter; Loibner, Hans; Himmler, Gottfried; Hricik, Donald E.; Heeger, Peter S.

doi:10.1097/01.tp.0000168148.56669.61

Cited by 19 publications

(15 citation statements)

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“…These technical limitations will be overcome as we (1) expand our stimulator population and (2) develop well-characterized and uniform populations of in vitro-expanded B cell stimulators (26) (rather than T cell-depleted PBL or spleen cells) for use in large-scale clinical trials. In addition, alterations in PRT reactivity may reflect environmental influence such as undiagnosed intercurrent infections, alterations in medications, recent immunizations, etc., that can influence significantly the cellular alloimmune response (27). None of the patients with significant alterations in PRT from our cohort has received a transplant, so we cannot comment on whether the detected alterations in PRT are clinically important.…”

Section: Discussionmentioning

confidence: 99%

Panel of Reactive T Cells as a Measurement of Primed Cellular Alloimmunity in Kidney Transplant Candidates

Poggio

Clemente

Hricik

et al. 2006

Journal of the American Society of Nephrology

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Pretransplantation panel reactive antibody (PRA) testing assesses posttransplantation risk for antibody-mediated graft injury. It was postulated analogously that screening for effector/memory alloreactive T cells by "panel of reactive T cells" (PRT) using IFN-␥ enzyme-linked immunosorbent spot assays would evaluate independently cellular alloimmunity in transplant candidates. Peripheral blood lymphocytes from 41 hemodialysis patients who were awaiting first renal transplants were tested against a panel of allogeneic stimulator cells. Positive assays were defined arbitrarily as >25 spots/300,000 peripheral blood lymphocytes, and positive PRT was defined as when the responder reacted to 40 or 75% (PRT-75) of the stimulators. Seventeen percent of patients were PRT-75؉, whereas 32% were PRA؉. Twelve percent of the cohort was PRT-75؉/PRA؊, and only 5% of the patients were PRA؉/PRT-75؉, indicating that T cell alloreactivity did not routinely imply B cell sensitization and vice versa. PRT-75؉ patients were more likely to be younger (<55 yr) and black. In contrast, a positive PRA was significantly associated with female gender but not race or age. Pretransplantation screening of cellular alloimmunity by enzyme-linked immunosorbent spot-based PRT detects a subset of hemodialysis patients who differ from those that are PRA؉. Preliminary correlations with posttransplantation outcome in seven recipients suggest that PRT screening has the potential to aid in risk assessment in renal transplant candidates.

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Section: Discussionmentioning

confidence: 99%

Panel of Reactive T Cells as a Measurement of Primed Cellular Alloimmunity in Kidney Transplant Candidates

Poggio

Clemente

Hricik

et al. 2006

Journal of the American Society of Nephrology

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“…HLA antibody production in a 16-year-old male donor was possibly due to vaccination, 19,20 because he had not been transfused or received transplantation. Among seven antibody-positive donors, two donors had HLA antibodies corresponding to recipient HLA antigens (recipient-specific antibodies, RSA).…”

Section: Hla Antibodies In Donorsmentioning

confidence: 99%

Donor-derived HLA antibody production in patients undergoing SCT from HLA antibody-positive donors

Taniguchi

Yoshihara

Maruya³

et al. 2012

Bone Marrow Transplant

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“…Theoretical concerns have been raised regarding the potential for triggering allograft rejection with vaccination (1,(18)(19)(20)(21)(22) (23)(24)(25) …”

Section: Its Efficiency In Immunodepressed Individuals Remains a Comentioning

confidence: 99%

Humoral and Cellular Immune Responses after Influenza Vaccination in Kidney Transplant Recipients

Candon

Thervet

Lebon

et al. 2009

American Journal of Transplantation

107

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Heterogeneous Alterations in Human Alloimmunity Associated with Immunization

Abstract: Therapeutic vaccinations can alter the strength of cellular and humoral alloimmunity in humans. The results suggest that serial immune monitoring of alloreactivity might be beneficial when immunizations are administered to potential transplant recipients.

Cited by 19 publications

References 20 publications

Panel of Reactive T Cells as a Measurement of Primed Cellular Alloimmunity in Kidney Transplant Candidates

Panel of Reactive T Cells as a Measurement of Primed Cellular Alloimmunity in Kidney Transplant Candidates

Donor-derived HLA antibody production in patients undergoing SCT from HLA antibody-positive donors

Humoral and Cellular Immune Responses after Influenza Vaccination in Kidney Transplant Recipients

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