Heterogeneity of twoBeauveria bassiana strains revealed by biochemical tests, protein profiles and bio-assays ofLeptinotarsa decemlineata (Col.: Chrysomelidae) andColeomegilla macultata lengi (Col.: Coccinellidae) larvae

Todorova, Silvia; Côté, Jean-Charles; Martel, Pierre; Coderre, Daniel

doi:10.1007/bf02372354

Cited by 26 publications

(21 citation statements)

References 18 publications

(7 reference statements)

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“…The carbohydrate utilization profiles of the two B. bassiana isolates also differed in their morphology, protein profiles and pathogenicity against Leptinotarsa decemlineata Say and Coleomegilla maculata lengi Timb. (Todorova et al 1994). Based on data obtained on the reactions of strains of Beauveria and Tolypocladium spp.…”

Section: Introductionmentioning

confidence: 99%

Utilization of Carbohydrates by Beauveria Bassiana Isolates Obtained from Forest Pests

Draganova¹,

Pilarska²,

Takov³

et al. 2011

Journal of Plant Protection Research

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Carbohydrate utilization profiles of ten isolates of the entomopathogenic fungus Beauveria bassiana (Bals.-Criv.) Vuillemin were studied and compared to assist in determining their phenotypic characterization. Isolates were obtained in pure cultures from dead individuals of several forest pests collected from different regions in Bulgaria. Studies on utilization profiles were based on the acidification of twenty carbohydrates.The results indicate that sucrose, maltose and trehalose were assimilated at a high degree compared to esculin, arabinose and dulcitol. According to the results of a cluster analysis of the carbohydrate utilization profiles, the B. bassiana isolates were divided into two larger groupings. All isolates in the first larger cluster were obtained from the coleopteran insects -Stenomax aeneus, Ips typographus, I. sexdentatus and Dryocoetes autographus. Isolates from the other cluster were obtained from the lepidopteran larvae of Thaumetopoea pityocampa and Lymantria dispar, and from an adult of the coleopteran species Hylurgops palliatus. We determined that each B. bassiana isolate exhibited a different and specific carbohydrate utilization profile but differences at the p-level < 0.05 were significant among some of them. The most distinguishable was the isolate 560Bb obtained from T. pityocampa. Differences between the isolate 560Bb and the other nine B. bassiana isolates were highly significant at the p-level < 0.005. Isolate 433Bb obtained from a dead adult of I. typographus was significantly different from five of the studied isolates at the p-level < 0.05.

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Section: Introductionmentioning

confidence: 99%

Utilization of Carbohydrates by Beauveria Bassiana Isolates Obtained from Forest Pests

Draganova¹,

Pilarska²,

Takov³

et al. 2011

Journal of Plant Protection Research

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“…Eight of the isolates showed close similarity while, highest distance matrix was recorded between 4 -37 and 4 -28 and 4 -39 and 4 -36 and lowest between isolates 4 -7 and 4 -14, 4 -7 and 4 -6 and between 4 -17 and 4 -14. Todorova et al [14] studied the heterogeneity of two isolates of entomopathogenic fungus B. bassiana using SDS -PAGE technique and the strains were characterized depending upon the protein profiles. Reineke and Zebitz [29] characterized the mycelial proteins of four isolates of B. brongniartii and reported a high level of intraspecific variation.…”

Section: Discussionmentioning

confidence: 99%

“…Todorova et al [14] studied the heterogeneity of two isolates of entomopathogenic fungus B. bassiana using SDS -PAGE techniques. Jordao et al [15] characterized five isolates of B. bassiana against Cosmopolites sordidus under laboratory and field conditions.…”

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confidence: 99%

Relationships among activities of extracellular enzyme production and virulence against Helicoverpa armigera in Beauveria bassiana

Kaur

Padmaja

2009

J. Basic Microbiol.

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Extracellular enzymes produced by Beauveria bassiana, are believed to play a key role in cuticle hydrolysis. Enzyme production and pathogenicity has been found to be positively correlated. Twenty-eight isolates of B. bassiana, collected from different geographical regions and host ranges were characterized by in vitro extracellular enzyme production and SDS-PAGE techniques for discerning biochemical basis for virulence among the different isolates. In vitro analysis of extracellular enzymes like protease, amylase, caseinase, chitinase and lipase was undertaken in an attempt to understand their relevance to virulence of the isolates. The different isolates of B. bassiana were evaluated for virulence to the second instar larvae of Helicoverpa armigera in laboratory bioassays. SDS-PAGE of total intracellular soluble proteins was also studied in order to understand affinities among the different isolates of B. bassiana. The relationship between enzyme production and pathogenicity and vice versa was nearly 50%. There was a 50% relationship associated with original insect host, pathogenicity and enzyme production.

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“…What is known about entomopathogens attacking coccinellids generally involves one of four situations: (1) rates of infection by nematodes or protozoa (Ceryngier & Hodek, 1996;Shapiro-Ilan & Cottrell, 2004); (2) mortality of overwintering populations (Iperti, 1966a, b;Ceryngier, 2000); (3) male-killing bacteria (Hurst & Jiggins, 2000); or (4) the effect of entomopathogens on non-target coccinellids (Magalhaes et al, 1988;Giroux et al, 1994;James & Lighthart, 1994;Todorova et al, 1994;James et al, 1995James et al, , 1998Pingel & Lewis, 1996;Poprawski et al, 1998;Cagá & Uhlík, 1999;Smith & Krischik, 2000;Todorova et al, 2000;Pell & Vandenberg, 2002).…”

Section: Introductionmentioning

confidence: 99%

Susceptibility of endemic and exotic North American ladybirds (Coleoptera: Coccinellidae) to endemic fungal entomopathogens

Cottrell¹,

Shapiro‐Ilan²

2008

Eur. J. Entomol.

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Abstract. We tested the laboratory susceptibility of four endemic and two exotic species of North American Coccinellidae to a single rate (2.5 × 10 5 conidia/ml) of Beauveria bassiana derived from different sources. The endemic species Olla v-nigrum, Cycloneda munda and Hippodamia convergens were susceptible to B. bassiana Isolate B which was originally isolated from O. v-nigrum and the rate assayed was known to represent the LC50 against O. v-nigrum. However, neither the endemic C. maculata nor the exotic species Harmonia axyridis and Coccinella septempunctata were susceptible to this isolate at this rate. Additionally, all species of endemic and exotic Coccinellidae used in these assays were resistant to B. bassiana strain GHA and BbAR1 at that rate. We discuss the implications of these results in regard to the establishment of exotic species in new habi tats.

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Heterogeneity of twoBeauveria bassiana strains revealed by biochemical tests, protein profiles and bio-assays ofLeptinotarsa decemlineata (Col.: Chrysomelidae) andColeomegilla macultata lengi (Col.: Coccinellidae) larvae

Cited by 26 publications

References 18 publications

Utilization of Carbohydrates by Beauveria Bassiana Isolates Obtained from Forest Pests

Utilization of Carbohydrates by Beauveria Bassiana Isolates Obtained from Forest Pests

Relationships among activities of extracellular enzyme production and virulence against Helicoverpa armigera in Beauveria bassiana

Susceptibility of endemic and exotic North American ladybirds (Coleoptera: Coccinellidae) to endemic fungal entomopathogens

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