Heterogeneity of nanomechanical properties of the human umbilical vein endothelial cell surface

Стародубцева, М. Н.; Надыров, Э. А.; Shkliarava, Nastassia M.; Tsukanava, Alena U.; Стародубцев, И. Е.; Kondrachyk, Aleksey N.; Matveyenkau, Matsvei V.; Недосейкина, М. С.

doi:10.1016/j.mvr.2021.104168

Cited by 2 publications

(8 citation statements)

References 37 publications

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“…The vimentin intermediate filament network formation in those zones was confirmed by the immunocytochemical method. Our data on the elastic modulus of both chemically fixed and living HUVECs support the remodeling of the actin cytoskeleton, which occurs simultaneously with the formation of the vimentin network; since changes in cell stiffness assessed using AFM are mainly associated with rearrangements of the cortical actin cytoskeleton in cells (Starodubtseva, 2022; Starodubtseva et al, 2021).…”

Section: Discussionsupporting

confidence: 63%

“…Overexpression of vimentin and some cell adhesion/migration relevant proteins was observed in cells upon epithelial-to-mesenchymal transition or malignant transformation (Lowery, Kuczmarski, Herrmann, & Goldman, 2015). It is known that TGF-β2 induces the formation of cross-linked actin networks which can be completely inhibited by inhibitors of TGF-β receptor, SMAD3, and ERK, as well as Our data on the elastic modulus of both chemically fixed and living HUVECs support the remodeling of the actin cytoskeleton, which occurs simultaneously with the formation of the vimentin network; since changes in cell stiffness assessed using AFM are mainly associated with rearrangements of the cortical actin cytoskeleton in cells (Starodubtseva, 2022;Starodubtseva et al, 2021).…”

Section: Effect Of Anti-cd109 Antibodies On Huvec's Mechanical Proper...supporting

confidence: 52%

“…The reorganization of the cytoskeleton leads to a change in HUVEC's mechanical properties. The heterogeneity in the nanomechanical parameters of HUVECs has been recently demonstrated by the AFM technique (Starodubtseva et al, 2021). Three different zones of HUVECs, the nuclear, perinuclear, and peripheral zones had relatively different mechanical properties (Starodubtseva et al, 2021).…”

Section: Effect Of Anti-cd109 Antibodies On Huvec's Mechanical Proper...mentioning

confidence: 99%

“…The heterogeneity in the nanomechanical parameters of HUVECs has been recently demonstrated by the AFM technique (Starodubtseva et al, 2021). Three different zones of HUVECs, the nuclear, perinuclear, and peripheral zones had relatively different mechanical properties (Starodubtseva et al, 2021). Figure 3 presents the AFM images recorded on three channels (topography, DMT modulus, and adhesion) of the microscale areas of the fixed HUVEC's surface for three experimental samples (control cells, cells after IgG treatment, and cells after IgG + anti-CD109 AB treatment).…”

Section: Effect Of Anti-cd109 Antibodies On Huvec's Mechanical Proper...mentioning

confidence: 99%

“…In our previous work, we showed that the mechanical property heterogeneity was inherent for endothelial cells (in the absence of mechanical stress), for example, the perinuclear zone was the softest zone of endothelial cells (Starodubtseva et al, 2021). Here, we revealed that anti-CD109 ABs altered the as described in a recently published report (Starodubtseva et al, 2021). Cells for atomic force microscopy (AFM) analysis were seeded in tissue culture dishes (Cell+, 35 Â 10 mm) for adherent cells with an adhesive coating (Sarstedt, Germany) and incubated for 2-4 days under standard cultivation conditions (5% CO 2 , 95% humidity, 37 C).…”

Section: Effect Of Anti-cd109 Antibodies On Huvec's Mechanical Proper...mentioning

confidence: 99%

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CD109‐regulated mechanical properties of endothelial cells

et al. 2023

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CD109 antigen on the endothelial cell surface plays an important role in vascular pathology. The aim of the work was to investigate the effect of the immobilization of CD109 antigen with specific antibodies on nanomechanical properties of human umbilical endothelial cells (HUVECs) using atomic force microscopy in quantitative nanomechanical property mapping mode (PeakForce QNM). Anti‐CD109 antibodies induced significant stiffening of the cell surface Me(LQ; UQ): in 1.45(1.07;2.29) times with respect to control cells for fixed cells and in 4.9(3.6;5.9) times with respect to control cells for living cells, and changes in the spatial distribution of cell surface mechanical properties. The changes in the HUVEC's mechanical properties were accompanied by the activation of the TGF‐/Smad2/3 signaling pathway and reorganization of the vimentin and actin cytoskeletal elements. Our findings show that blocking CD109 antigen using anti‐CD109 antibodies leads in HUVECs to the processes similar to that occur after cell TGF‐β‐signaling activation. Therefore, we suggest that CD109 antigen may be involved in regulating the mechanical behavior of endothelial cells.

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Section: Discussionsupporting

confidence: 63%

Section: Effect Of Anti-cd109 Antibodies On Huvec's Mechanical Proper...supporting

confidence: 52%

Section: Effect Of Anti-cd109 Antibodies On Huvec's Mechanical Proper...mentioning

confidence: 99%

Section: Effect Of Anti-cd109 Antibodies On Huvec's Mechanical Proper...mentioning

confidence: 99%

Section: Effect Of Anti-cd109 Antibodies On Huvec's Mechanical Proper...mentioning

confidence: 99%

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CD109‐regulated mechanical properties of endothelial cells

et al. 2023

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Mechanical Properties and Nanomotion of BT-20 and ZR-75 Breast Cancer Cells Studied by Atomic Force Microscopy and Optical Nanomotion Detection Method

Starodubtseva,

Shkliarava,

Chelnokova

et al. 2023

Cells

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Cells of two molecular genetic types of breast cancer—hormone-dependent breast cancer (ZR-75 cell line) and triple-negative breast cancer (BT-20 cell line)—were studied using atomic force microscopy and an optical nanomotion detection method. Using the Peak Force QNM and Force Volume AFM modes, we revealed the unique patterns of the dependence of Young’s modulus on the indentation depth for two cancer cell lines that correlate with the features of the spatial organization of the actin cytoskeleton. Within a 200–300 nm layer just under the cell membrane, BT-20 cells are stiffer than ZR-75 cells, whereas in deeper cell regions, Young’s modulus of ZR-75 cells exceeds that of BT-20 cells. Two cancer cell lines also displayed a difference in cell nanomotion dynamics upon exposure to cytochalasin D, a potent actin polymerization inhibitor. The drug strongly modified the nanomotion pattern of BT-20 cells, whereas it had almost no effect on the ZR-75 cells. We are confident that nanomotion monitoring and measurement of the stiffness of cancer cells at various indentation depths deserve further studies to obtain effective predictive parameters for use in clinical practice.

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Heterogeneity of nanomechanical properties of the human umbilical vein endothelial cell surface

Cited by 2 publications

References 37 publications

CD109‐regulated mechanical properties of endothelial cells

CD109‐regulated mechanical properties of endothelial cells

Mechanical Properties and Nanomotion of BT-20 and ZR-75 Breast Cancer Cells Studied by Atomic Force Microscopy and Optical Nanomotion Detection Method

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