Heterogeneity of Marine Birnaviruses Isolated from Turbot(Scophthalmus maximus).

Novoa, Beatriz; Figueras, António

doi:10.3147/jsfp.31.145

Cited by 7 publications

(9 citation statements)

References 23 publications

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“…MABV Y-6 was also homologous with other MABV isolates from several marine fish in Japan (Kusuda et al, 1993). Therefore, birnavirus from various marine fish in coastal areas of Korea and Japan are serologically homologous, in contrast to other studies in Europe (Novoa and Figueras, 1996;Cutrin et al, 2000). Nishizawa et al (2005) also suggested that aquabirnavirus isolated from marine fish in Japan were indigenous to Japan, based on a phylogenetic analysis.…”

Section: Nc1mentioning

confidence: 80%

“…The results indicate that these isolates are serologically homologous. Novoa and Figueras (1996) reported that six marine birnaviruses isolated from turbot Scophthalmus maximus in a restricted geographic area off the coast of Galicia (northwestern Spain) revealed a high heterogeneity of nucleotide sequences and polypeptide electrophoretic patterns and their reactions with the monoclonal antibodies. In addition, the turbot isolates were not exactly the same as the reference strains of IPNV Ab, Sp, and VR-299.…”

Section: Nc1mentioning

confidence: 99%

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Distribution of marine birnavirus in cultured olive flounder Paralichthys olivaceus in Korea

et al. 2008

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Surveys of marine birnavirus (MABV) were undertaken in cultured olive flounder Paralichthys olivaceus from the south and west coastal areas and Jeju in Korea during the period January 1999 to April 2007. MABV was detected in all seasons from the fry, juveniles and adult fish from the areas examined. Evident cytopathic effects of the virus including rounding and cell lysis were observed in chinook salmon embryo (CHSE-214) and rainbow trout gonad (RTG-2) cells, but not in fathead minnow (FHM) and epithelial papilloma of carp (EPC) cells. Nucleotide sequences of the VP2/NS junction region of the Korean isolates showed 97.8% ~ 100% similarity, and they belonged to the same genogroup. Cross neutralization tests with serotype-specific rabbit antisera against MABV strains exhibited a close antigenic relationships between strains, and were distinct from infectious pancreatic necrosis virus (IPNV) strains. Coinfection of MABV with bacteria (Streptococcus iniae, Vibrio spp.) and viruses (nervous necrosis virus, lymphocystis disease virus, viral hemorrhagic septicemia virus) was observed.

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Section: Nc1mentioning

confidence: 80%

Section: Nc1mentioning

confidence: 99%

Distribution of marine birnavirus in cultured olive flounder Paralichthys olivaceus in Korea

et al. 2008

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“…Virus. Four reference strains of birnaviruses (Sp, Ab, WB, and the Norwegian serotype N1) and six birnaviruses isolated from turbot (306, 311, 390, 231, 160, and 460) were used (21). Stock viruses were prepared in the CHSE-214 cell line derived from chinook salmon (17).…”

Section: Methodsmentioning

confidence: 99%

“…The main properties of these MAbs are summarized in Table 1. The immunodot assay was performed as previously described (21). Briefly, the viruses, with a minimum titer of 10 7 50% tissue culture infective doses per ml were added to a hydrated nitrocellulose membrane in a Multiwell BioDot system (Bio-Rad) and incubated for 1 h at room temperature.…”

Section: Methodsmentioning

confidence: 99%

“…Since 1988, several birnaviruses have been isolated from this marine fish. High heterogeneity among these strains was detected on the basis of structural characteristics and immunodot analysis with four monoclonal antibodies (MAbs) (21). Serotyping of new isolates of aquatic birnaviruses is still usually accomplished by neutralization with reference polyclonal antisera.…”

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confidence: 99%

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Comparison of different procedures for serotyping aquatic birnavirus

Novoa

Blake

Nicholson

et al. 1995

Appl Environ Microbiol

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The current classification of aquatic birnaviruses is based on seroneutralization assays with polyclonal antibodies. In this study a comparison of several procedures used for serotyping aquatic birnaviruses was made with 10 virus strains (4 reference strains from salmonids and 6 birnaviruses isolated from turbot [Scophthalmus maximus]). The relationships among the birnavirus strains were studied by seroneutralization assay with polyclonal antibodies and by immunodot assay with both polyclonal and monoclonal antibodies. The results were compared with a presumptive classification obtained from analysis of restriction enzyme patterns of cDNA products obtained by PCR amplification. No correlation was found among the results obtained by the different procedures. The seroneutralization and the immunodot assays with polyclonal antibodies were not useful in classifying these birnavirus strains; however, patterns of reaction with monoclonal antibodies emphasized the individuality of the strains, particularly in the case of two strains (231 and 460) whose patterns did not correspond to established serotypes. The application of PCR and restriction enzyme analysis is a promising system for approaching the classification of this viral group on the basis of genomic differences and similarities. The variable results obtained in this comparison lead us to think that the current classification of aquatic birnavirus may not be the most accurate and there is a need for modification incorporating recent isolates, not only from salmonid species but also from marine fish.

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Trout and Salmon Viruses

Plumb

Hanson

2010

Health Maintenance and Principal Microbial Diseases of Cultured Fishes

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Heterogeneity of Marine Birnaviruses Isolated from Turbot(Scophthalmus maximus).

Cited by 7 publications

References 23 publications

Distribution of marine birnavirus in cultured olive flounder Paralichthys olivaceus in Korea

Distribution of marine birnavirus in cultured olive flounder Paralichthys olivaceus in Korea

Comparison of different procedures for serotyping aquatic birnavirus

Trout and Salmon Viruses

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