Heterogeneity of intraepithelial lymphocytes in refractory sprue: potential implications of CD30 expression

In, Farstad; Fe, Johansen; Vlatkovic, Ljiljana; Jahnsen, Jørgen; Scott, Helge; Fausa, O; Bjørneklett, A; Brandtzæg, Per; Ts, Halstensen

doi:10.1136/gut.51.3.372

Cited by 59 publications

(44 citation statements)

References 24 publications

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“…Clinical, biological and histological manifestations All 32 patients (100%) had diarrhoea, with a median number of 8 liquid stools per day [2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20]. Twenty-four patients (75%) had abdominal pain, which was rarely severe (n = 3).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Olmesartan-associated enteropathy: results of a national survey

Marthey

Cadiot

Seksik

et al. 2014

Aliment Pharmacol Ther

174

171

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Section: Resultsmentioning

confidence: 99%

“…The median cumulative hospital stay was 29 days . Four patients required intensive care (12 days [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22]). Seven patients required enteral nutrition (30 days ) and 10 required parenteral nutrition (29 days ).…”

Section: Outcomementioning

confidence: 99%

Olmesartan-associated enteropathy: results of a national survey

Marthey

Cadiot

Seksik

et al. 2014

Aliment Pharmacol Ther

174

171

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“…In this disease, a massive expansion of IELs is observed with an unusual phenotype presenting with intracellular CD3, but lacking surface CD3-TCR complexes, often lacking CD8 and CD4, and a variable expression of CD94 (41)(42)(43)(44). Such IELs are considered neoplastic and have been suggested to represent a cryptic variant of enteropathy-associated T cell lymphoma (45).…”

Section: Discussionmentioning

confidence: 99%

Phenotypic Characterization of CD3−7+ Cells in Developing Human Intestine and an Analysis of Their Ability to Differentiate into T Cells

Günther

Holloway

Gordon

et al. 2005

The Journal of Immunology

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We have identified a large population of CD3−7+ cells in human fetal gut. Three- and four-color flow cytometry revealed a distinct surface Ag profile on this population; the majority were negative for CD4 and CD8, whereas most of the remainder expressed the CD8αα homodimer. In contrast about half of CD3+ cells expressed CD4 and half expressed CD8α. A large proportion of CD3−7+ cells expressed CD56, CD94, and CD161, and whereas CD3+ T cells also expressed CD161, they only rarely expressed CD56 or CD94. Further studies were conducted to determine whether the CD3−7+ cells have the potential to differentiate into CD3+ cells. About half of CD3−7+ cells contain intracellular CD3ε. Rearranged TCR γ-chains were detected in highly purified CD3−7+ cells as an early molecular sign of T cell commitment, and the pattern of rearrangement with V regions spliced to the most 5′ Jγ segment is reminiscent of early thymocyte differentiation. In reaggregate thymic organ cultures, CD3−7+ cells also gave rise to CD3+ T cells. Thus, we demonstrate that the CD3−7+ cells present in the human fetal gut display a distinct phenotype and are able to develop into CD3+ T cells.

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“…2 IELs in vivo, the RCD cell lines also express CD30 and DNAM-1, both uniformly expressed on RCD-associated lymphoma cells (25) (Fig. 7C).…”

Section: Discussionmentioning

confidence: 91%

DNAM-1 Mediates Epithelial Cell-Specific Cytotoxicity of Aberrant Intraepithelial Lymphocyte Lines from Refractory Celiac Disease Type II Patients

Tjon

Kooy–Winkelaar

Tack³

et al. 2011

The Journal of Immunology

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In refractory celiac disease (RCD), intestinal epithelial damage persists despite a gluten-free diet. Characteristic for RCD type II (RCD II) is the presence of aberrant surface TCR-CD3− intraepithelial lymphocytes (IELs) that can progressively replace normal IELs and eventually give rise to overt lymphoma. Therefore, RCD II is considered a malignant condition that forms an intermediate stage between celiac disease (CD) and overt lymphoma. We demonstrate in this study that surface TCR-CD3− IEL lines isolated from three RCD II patients preferentially lyse epithelial cell lines. FACS analysis revealed that DNAM-1 was strongly expressed on the three RCD cell lines, whereas other activating NK cell receptors were not expressed on all three RCD cell lines. Consistent with this finding, cytotoxicity of the RCD cell lines was mediated mainly by DNAM-1 with only a minor role for other activating NK cell receptors. Furthermore, enterocytes isolated from duodenal biopsies expressed DNAM-1 ligands and were lysed by the RCD cell lines ex vivo. Although DNAM-1 on CD8+ T cells and NK cells is known to mediate lysis of tumor cells, this study provides, to our knowledge, the first evidence that (pre)malignant cells themselves can acquire the ability to lyse epithelial cells via DNAM-1. This study confirms previous work on epithelial lysis by RCD cell lines and identifies a novel mechanism that potentially contributes to the gluten-independent tissue damage in RCD II and RCD-associated lymphoma.

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Heterogeneity of intraepithelial lymphocytes in refractory sprue: potential implications of CD30 expression

Cited by 59 publications

References 24 publications

Olmesartan-associated enteropathy: results of a national survey

Olmesartan-associated enteropathy: results of a national survey

Phenotypic Characterization of CD3−7+ Cells in Developing Human Intestine and an Analysis of Their Ability to Differentiate into T Cells

DNAM-1 Mediates Epithelial Cell-Specific Cytotoxicity of Aberrant Intraepithelial Lymphocyte Lines from Refractory Celiac Disease Type II Patients

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