Heterogeneity of human natural killer cell populations

Kall, Mary Ann; Koren, Hillel S.

doi:10.1016/0008-8749(78)90315-5

Cited by 63 publications

(15 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results obtained in this study suggest that the non-T cell fraction (which we assume contains B cells and Fc receptor-positive non-B cells) is the most active one on a per cell basis. These findings are in accordance with our results in humans (20) as well as with those obtained by several other authors (21)(22)(23)(24). Further characterization of the effector cells by using other techniques based on Fc receptors and surface Ig should be informative.…”

supporting

confidence: 93%

Natural killing and antibody-dependent cellular cytotoxicity are independent immune functions in the Minnesota miniature swine.

Koren¹,

Amos²,

Kim³

1978

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Peripheral blood lymphocytes from Minnesota miniature pigs were tested for natural killing (NK) and antibody-dependent cellular cytotoxicity (ADCC) in a 2-to 4-hr 5tCr release assay against human myeloid and lymphoid tumor target cells. Adult specific pathogen-free and germfree animals exhibited normal levels of activity in both assays. In addition, the NK and ADCC activities of peripheral blood lymphocytes from colostrum-deprived newborn piglets were examined. These animals were obtained by hysterectomy and previously shown to be immunologically "virgin." We found that these newborn piglets exhibited normal ADCC but lacked NK activity. The differences in the ontogeny of the two activities suggest that they are distinct. Preliminary effector cell characterization studies suggest that: (i) NK and ADCC in the pig are physically not separable; (ii) the majority of the cytotoxic activity on a cell-per-cell basis is mediated by the non-T lymphocyte fraction; and (iii) the rosetted T cells, which account for about 60% of the total pig peripheral blood lymphocytes, have low but demonstrable cytotoxic activity as well.

show abstract

supporting

confidence: 93%

Natural killing and antibody-dependent cellular cytotoxicity are independent immune functions in the Minnesota miniature swine.

Koren¹,

Amos²,

Kim³

1978

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…They are referred to as large granular lymphocytes (LGL) (Saksela et al, 1979a). Fc R+ ADCC activity against the SB target cell is associated with FcR-bearing E RFC cells, including cells of the NK class of lymphocytes (Kall & Koren, 1978 (LGL) were present in the patient blood (Table III) they were not active. Because of the low activity in blood in the presence of the marker, the absence of both activity and the LGL marker from the ascites fraction need not be related.…”

Section: Association Of Cell Markers With Effector-cell Activitymentioning

confidence: 99%

Mononuclear-cell infiltration in ovarian cancer. II. Immune function of tumour and ascites-derived inflammatory cells

Haskill¹,

Koren²,

Becker³

et al. 1982

Br J Cancer

View full text Add to dashboard Cite

Summary.-Mononuclear cell fractions were isolated from blood, ascites and solid tumours of patients undergoing surgery for Stages III and IV PHA responses of patient blood and ascites fractions were about half that of normal blood. Tumour-infiltrating lymphocytes (TIL) were less than 10% as responsive as normal blood. The depressed PHA responses of the TIL were not due to the presence of a suppressor cell population. NK activity of patient blood was less than that of normal blood, but not as much as the ascites or TIL cells. The activity of the ascites-derived lymphocytes was enhanced by treatment with interferon. ADCC activity against both CRBC and SB cells was normal or higher than controls in patient blood, and depressed in the ascites-derived fractions. TIL responded to <10% of the patient blood values.The results indicate a lack of response by ascitic and TIL cells in assays dependent on FcR -bearing effector cells and a greater loss of PHA-reactive cells from the tumour than from blood and ascites. These data could result from intratumour inactivation, or a failure of the particular subset to localize either In the ascites or the tumour site.

show abstract

“…While it has been claimed that human and rat NK cells are Fc-receptor (for IgG) bearing subpopulations of T cells (Kay et al, 1977) Kall & Koren (1978 have shown that the most active NK cells in humans are non-T cells which do not adhere to nylon-wool columns. There is no information available to date (see review by Kiessling & Wigzell, 1979) which would allow us to compare the specificity of NK cells with the cytostatic effector cells shown here, which (see Fig.…”

Section: Specificity Of Effector Cells In Cytostasis Andmentioning

confidence: 99%

Inhibition of cell proliferation rather than of cell lysis as a measure of immune reactivity in embryo-antigen-challenged mice

Gorczynski¹,

MacRae²

1981

Br J Cancer

View full text Add to dashboard Cite

An assay system is described in which effector cells added along with suitable target cells inhibit, in a quantitative fashion, the subsequent uptake of 3H-thymidine by those target cells. Effector cells active in this assay, using embryonic fibroblast cells as targets, develop spontaneously in cultures of mouse lymphoid cells, but are apparently different from those described earlier by investigators of activity in cytotoxic assays. Further evidence is presented to show the development of spleen-derived effector cells with cytostatic activity (for embryonic fibroblast target cells) in mice during the course of normal pregnancy, or growth of spontaneously appearing mammary adenocarcinomas. Indeed, such effector cells can also be found within the growing solid mass itself. Different populations of tumour cells isolated from a solid tumour apparently differ in their susceptibility to growth inhibition by tumour-bearer-derived cytostatic effector cells, a phenomenon which may be related to metastatic spread of tumour cells.

show abstract

Heterogeneity of human natural killer cell populations

Cited by 63 publications

References 30 publications

Natural killing and antibody-dependent cellular cytotoxicity are independent immune functions in the Minnesota miniature swine.

Natural killing and antibody-dependent cellular cytotoxicity are independent immune functions in the Minnesota miniature swine.

Mononuclear-cell infiltration in ovarian cancer. II. Immune function of tumour and ascites-derived inflammatory cells

Inhibition of cell proliferation rather than of cell lysis as a measure of immune reactivity in embryo-antigen-challenged mice

Contact Info

Product

Resources

About