Heterogeneity of human blood monocytes: the CD14+CD16+ subpopulation

Ziegler‐Heitbrock, H. W. L.

doi:10.1016/0167-5699(96)10029-3

Cited by 278 publications

(252 citation statements)

References 49 publications

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“…They promptly expand in HIV infection and during sepsis. 25,26 Consistent with their ability of being major producers of TNFa 27 and minimal producers of IL-10, 28 they are powerful proinflammatory cells. They are CD64 weakly positive and CD11b low .…”

Section: Discussionmentioning

confidence: 88%

Phenotype and function of a CD56+ peripheral blood monocyte

et al. 2004

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G-CSF primed CD34 cells cultured for 2-3 weeks in IL-2 and stem cell factor generate CD56 high cells with phenotypic and morphologic features of NK cells, and a novel adherent CD56 low CD16À population expressing myeloid markers (CD33 and HLA-DR). We hypothesized that similar cells might also occur in peripheral blood. In 13/13 normal individuals, we found a circulating population of CD56 low , CD33 þ , FccRI þ , FccRII þ , HLA-DR þ , CD11b high , CD14 þ monocytes closely resembling the cultured CD56 low CD33 þ cells. They may represent a normal counterpart of the CD56 þ CD33 þ hybrid myeloid/natural killer cell leukemia. Their mean frequency was 1.371% (standard deviation), range 0.16-3.5%, of total mononuclear cells. CD56 low CD33 þ cells, primed with cytomegalovirus antigen, induced autologous T-lymphocyte proliferation comparably to CD56À, CD14 þ peripheral blood monocytes (PBM). Conversely, CD56 low cells induced greater T-cell proliferation than CD56À PBM when lymphocyte responders were HLA mismatched. Unstimulated CD56 low CD33 þ cells showed a low antiproliferative effect on K562, which was increased upon LPS stimulation. The pattern of cytokine production by CD56 low CD33 þ cells and PBM largely overlapped; however, they produced detectable levels of IL-6 and IL-1b. These results define a minor monocyte population with distinct phenotypic and functional features.

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Section: Discussionmentioning

confidence: 88%

Phenotype and function of a CD56+ peripheral blood monocyte

et al. 2004

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“…monocyte-related subpopulation (CD14 -/low CD16 ? DC) initially described as a minor subset of mature monocytes [13][14][15]. Accordingly, this latter DC subpopulation seems to display intermediate features between a PB monocyte and DC, such as a high expression of FccRIII (CD16), a relatively low activity as APC in comparison with other peripheral blood DC and a high ability to generate in vitro activation of naive T cells.…”

Section: Introductionmentioning

confidence: 99%

Functional characterization of peripheral blood dendritic cells and monocytes in systemic lupus erythematosus

Henriques¹,

Inês

Carvalheiro³

et al. 2011

Rheumatol Int

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With the purpose of contributing to a better knowledge of the APCs functional activity in SLE, we evaluated the distribution and functional ability to produce pro-inflammatory cytokines (TNF-a, IL-1b, IL-6 and IL-12) of peripheral blood (PB) monocytes and DC (tDC), particularly myeloid (mDC) and CD14 -/low CD16 ? DC subpopulations comparing them with those obtained from healthy individuals. The study was performed in 34 SLE patients with diverse disease activity scores (SLEDAI) and 13 healthy age-and sex-matched controls (NC). Our results show an overall decrease in absolute number and relative frequency of tDC in SLE patients with active disease when compared to those with inactive disease and NC, although this decrease did not seem to have an effect on the distribution of PB DC subsets. The monocytes number in SLE patients was similar to those found in NC, whereas a higher frequency of monocytes producing cytokines as well as the amount of each cytokine per cell found without stimulation was particularly observed in those patients with active disease. After stimulation, we observed a higher frequency of IL-12-producing monocytes in active SLE patients. On the other hand, we found among DCs higher frequencies of cytokine-producing CD14 -/low CD16 ? DCs and a higher amount of cytokines produced per cell, particularly in active disease. These findings support an increased production of inflammatory cytokines by APCs in active SLE, mostly associated with alterations in CD14 -/low CD16 ? DC subset homeostasis that might contribute to explain the dynamic role of these cells in disease pathogenesis.

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“…Interestingly, about 50% of DC-SIGN ϩ blood cells stained positive for CD16, which is expressed on a subset of monocytes with APC function. 22 Phenotypic comparison with both monocytes and monocyte-derived immature DCs showed that DC-SIGN ϩ blood cells display a unique expression pattern that seems like an intermediate cell type between monocyte and immature DC ( Figure 3A and Table 1). …”

Section: Phenotypic Analysis Of Dc-sign ؉ Blood Cellsmentioning

confidence: 99%