Heterogeneity of Genetic Damage in Cervical Nuclei and Lymphocytes in Women with Different Levels of Dysplasia and Cancer-Associated Risk Factors

Alvarez-Moya, Carlos; Reynoso-Silva, Mónica; Canales-Aguirre, Alejandro A.; Chavez-Chavez, José O.; Castañeda-Vázquez, Hugo; Feria‐Velasco, Alfredo

doi:10.1155/2015/293408

Cited by 5 publications

(3 citation statements)

References 37 publications

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“…Recently our group (18) used migration groups (MG) as an alternative parameter to evaluate genetic damage based on the heterogeneity of the migration of comet cells. Previous studies have reported great heterogeneity of damage in the DNA of comet cells due to variations in cell size, differences in sensitivity to genotoxic or differences in the type of genetic damage manifested (19)(20)(21). The severity of cytotoxicity and mutagenicity caused by UV radiation in mammalian cells depends on the lesions produced mainly on the DNA (22).…”

Section: Introductionmentioning

confidence: 99%

Genetic damage in human blood cells exposed to germicidal lamps and cytoprotection of ascorbic acid

Reynoso-Silva,

Alvarez-Moya,

Vargas-Radillo

et al. 2024

revbiomed

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Introduction. Germicidal lamps have a wavelength range of 200-280 nm and can affect the integrity of the DNA of people who handle this equipment. Human lymphocytes are excellent biomonitors of genetic damage and widely used with the comet assay. Objective. Evaluation of genotoxicity in human blood cells exposed to UV-C radiation (254 nm) emitted by germicidal lamps and the cytoprotective effect of ascorbic acid, using the comet test. Material and methods. Slides containing lymphocytes immersed in agarose gel were exposed to UV-C radiation (254 nm) for periods of 5, 10 and 15 minutes and 70 cm away. The antigenotoxic effect was determined in cells exposed to UV-C for 5 minutes and 70 cm away, subsequently the slides were subjected to an ascorbic acid solution for periods of 5, 10 and 15 for two hours. In both situations, genetic damage was quantified by the comet test using three parameters: tail length, tail moment, and migration groups. Results. The three parameters detected significant genotoxic activity (p<0.05) in the times of exposure to UV-C and cytoprotective effect of ascorbic acid (p<0.05). Conclusions. The handling of UV-C germicidal lamps is often wrong and dangerous to exposed people or organisms. These data suggest that ascorbic acid increases DNA protection in cells exposed to UV-C radiation. Keyword: UV radiation, genetic damage, genotoxicity, ascorbic acid, comet assay.

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Section: Introductionmentioning

confidence: 99%

Genetic damage in human blood cells exposed to germicidal lamps and cytoprotection of ascorbic acid

Reynoso-Silva,

Alvarez-Moya,

Vargas-Radillo

et al. 2024

revbiomed

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“…These studies indicated that the heterogeneity of genetic damage could reflect the variation of DNA lesions from one cell to another in the same population [14]. The heterogeneity in DNA migration allowed the observation of responses of small subpopulations to various treatments [12,15] as well as its relationship with cell size, differences in sensitivity to genotoxic agents, or differences in the type of manifested genetic damage [2,10,[15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Genetic Damage and Antigenotoxic Effect of Ascorbic Acid in Erythrocytes of Orochromis niloticus and Ambystoma mexicanum Using Migration Groups as a Parameter

et al. 2022

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The comet assay system is an efficient method used to assess DNA damage and repair; however, it currently provides the average result and, unfortunately, the heterogeneity of DNA damage loses relevance. To take advantage of this heterogeneity, migration groups (MGs) of cell comets can be formed. In this study, genetic damage was quantified in erythrocytes of Oreochromis niloticus and Ambystoma mexicanum exposed to ethyl methanesulfonate (ethyl methanesulfonate (EMS) 2.5, 5, and 10 mM over two hours) and ultraviolet C radiation (UV-C) for 5, 10, and 15 min using the tail length, tail moment, and migration group parameters. Additionally, blood cells were exposed to UV-C radiation for 5 min and treated post-treatment at 5, 10, and 15 mM ascorbic acid (AA) for two hours. With the MG parameter, it was possible to observe variations in the magnitude of genetic damage. Our data indicate that MGs help to detect basal and induced genetic damage or damage reduction with approximately the same efficiency of the tail length and tail moment parameters. MGs can be a complementary parameter used to assess DNA integrity in species exposed to mutagens.

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“…Similarly,Udumudi et al., Carlos Alvarez-Moya et al, and Gandhi et al found that cervical cancer patients exhibited higher levels of DNA damage (comet tail length) than age-matched controls. Udumudi et al and Carlos Alvarez-Moya et al studied DNA damage in different stages of cervical cancer and discovered that DNA damage increased (comet tail length) as the stage of cervical cancer grew[34,48,58]. Smitha et al investigated the impact of MDA, an oxidative stress marker, in cervical cancer patients.…”

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confidence: 99%

Deoxyribose Nucleic Acid Damage and Its Association With Plasma Malondialdehyde Levels Among Patients With Cervical Cancer: A Case-Control Study

SSSN,

Chaturvedula

et al. 2024

Cureus

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Purpose The objective of this research project was to estimate DNA damage in patients diagnosed with cervical cancer using the comet assay, establish a correlation between this quantification and the oxidative stress marker malondialdehyde (MDA; plasma MDA), and compare the resulting parameters between the cases and age-matched controls. Materials and methods This study included 49 cervical cancer cases and 49 age-matched controls to measure DNA damage parameters such as comet length, head diameter, percentage of DNA in the comet head, tail length, percentage of DNA in the comet tail, and oxidative stress marker (plasma MDA) using the thiobarbituric acid reactive substance (TBARS) enzyme-linked immunosorbent assay (ELISA) method. Results Comet metrics suggesting DNA damage, such as comet length, tail length, and percentage of DNA in the comet tail, were considerably higher in cervical cancer cases than in age-matched controls. The proportion of DNA in the comet head, representing undamaged/mild DNA damage, was significantly higher in age-matched controls than in cervical cancer patients. Plasma MDA and comet tail length were shown to have a positive correlation. Compared to the age-matched controls, those between the ages of 30 and 39, with a parity of two to four, who had a history of early age at first pregnancy and a positive family history of cervical cancer, had the highest level of DNA damage. Conclusion The elevated levels of comet parameters and their positive correlation with plasma MDA suggest that individuals diagnosed with cervical cancer have a higher degree of DNA damage compared to the control group. In conjunction with established methods like the PAP smear, this predictive test comprising comet assay and estimation of plasma MDA may be utilized to identify and assess the risk of cervical cancer in individuals aged 30-39 years, with a parity between two and four pregnancies and a prior history of early age at first pregnancy, accompanied by a positive family history of the disease.

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Heterogeneity of Genetic Damage in Cervical Nuclei and Lymphocytes in Women with Different Levels of Dysplasia and Cancer-Associated Risk Factors

Cited by 5 publications

References 37 publications

Genetic damage in human blood cells exposed to germicidal lamps and cytoprotection of ascorbic acid

Genetic damage in human blood cells exposed to germicidal lamps and cytoprotection of ascorbic acid

Evaluation of Genetic Damage and Antigenotoxic Effect of Ascorbic Acid in Erythrocytes of Orochromis niloticus and Ambystoma mexicanum Using Migration Groups as a Parameter

Deoxyribose Nucleic Acid Damage and Its Association With Plasma Malondialdehyde Levels Among Patients With Cervical Cancer: A Case-Control Study

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