Heterogeneity of Epithelial Cells and Reactive Components in Thymomas: An Ultrastructural and Immunohistochemical Study

Eimoto, Tkdaaki; Teshima, Kohichi; Shirakusa, T; Takeshita, Morishige; Okamura, Hideki; Naito, Hideaki; Mitsui, Tetsuji; Kikuchi, Masahiro

doi:10.3109/01913128609014592

Cited by 27 publications

(10 citation statements)

References 26 publications

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“…This swith function from proapoptotic activity to cell-migration and motility-promoting might be correlated with DAP3 overexpression advanced thymoma. Human thymoma is a peculiar epithelial neoplasm where abnormal epithelium supports the differentiation of polyclonal T-lymphocytes [27][28][29][30]. The immunophenotypic studies have shown that most of the lymphocytes in thymoma are identical to normal cortical thymocytes [27][28][29].…”

Section: Discussionmentioning

confidence: 98%

Arg and DAP3 expression was correlated with human thymoma stage

Sasaki

Ide

Yukiue

et al. 2004

Clin Exp Metastasis

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Thymoma is one of the most common solid tumors in the mediastinum. As there is no typical cell line for human thymoma, the development and use of molecular-based therapy for thymoma will require detailed molecular genetic analysis of patients' tissues. The recent reports showed that the gain of chromosome 1q regions was frequent in thymoma. We investigated the use of oligonucleotide arrays to monitor in vivo gene expression levels at chromosome 1q regions in the early- (stage I or II) and late-stage (stage IVa) thymoma tissues from patients. These in vivo gene expression profiles were verified by real-time quantitative reverse transcription polymerase chain reaction (RT-PCR) using LightCycler for 36 thymoma patients. Using both the methods, candidate genes were come up. These are Arg tyrosine kinase and death-associated protein 3 (DAP3), which are known as ionizing radiation resistance conferring proteins. The Arg/GAPDH mRNA level in stage IV thymoma (90.716 +/- 177.851) was significantly higher than in stage I thymoma (10.335 +/- 25.744, P = 0.0465). The DAP3/GAPDH mRNA level in stage IV thymoma (17.424 +/- 20.649) was significantly higher than in stage I thymoma (5.184 +/- 3.878, P = 0.0305). DAP3 expression was also correlated with the WHO classification. The combined use of oligonucleotide microarray and real-time RT-PCR analyses provided a candidate molecular marker surrounding the development and progression of thymoma correlated with chromosome 1q.

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Section: Discussionmentioning

confidence: 98%

Arg and DAP3 expression was correlated with human thymoma stage

Sasaki

Ide

Yukiue

et al. 2004

Clin Exp Metastasis

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“…To date, immunohistochemical studies using various epithelial markers could not detect any definite differences between medullary and cortical type thymomas [4,10,22,25,27,32]. There were only two studies using PE-35 in cryostat sections of thymomas to characterize the tumor cells.…”

Section: Discussionmentioning

confidence: 99%

PE-35-related antigen expression and CD1a-positive lymphocytes in thymoma subtypes based on Müller-Hermelink classification

et al. 2000

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PE-35 monoclonal antibody, detecting a cell-surface antigen of various types of carcinoma and normal epithelium, reacts exclusively with the medullary epithelium in the thymus; therefore, the antigen has been considered as a marker of medullary differentiation in thymomas. Using the catalyzed signal amplification method, which made it possible to apply PE-35 to routinely processed, archival tissues, we examined expression of this antigen, together with CD1a reactivity of lymphocytes, in 40 thymic epithelial tumors subclassified using the Mü1ler-Hermelink system. Medullary thymomas infiltrated with a small number of CD1a-negative lymphocytes were PE-35 positive, although many of the long spindle tumor cells were PE-35 negative. Mixed thymomas and predominantly cortical thymomas, both with prominent CD1a-positive lymphocytes, were also PE-35 positive, although some areas of the latter type were PE-35 negative. Cortical thymomas with decreased numbers of CD1a-positive lymphocytes were largely PE-35 negative. In well-differentiated thymic carcinomas with a few CD1a-positive lymphocytes, two cases were negative, but four cases were at least focally positive with PE-35. All high-grade thymic carcinomas infiltrated with some CD1a-negative lymphocytes were PE-35 positive. These results suggested that medullary thymoma generally possesses the medullary nature, although the latter tends to be lost in the long spindle tumor cells. Mixed and predominantly cortical thymomas may have mixed medullary phenotype and cortical function. Cortical thymoma and many well-differentiated thymic carcinomas may possess the cortical nature, while the large polygonal tumor cells tend to lose immature T-lymphocyte-retaining function.

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“…[27][28][29] It has already been demonstrated by conventional histology that thymomas contain lymphocytes with a stimulated or activated appearance, and some mitoses are also seen. [27][28][29] However, in our study, neither mRNA nor protein level of GPI-80 in thymoma correlated with lymphocyte infiltration of thymoma. Thus, we believe that expression of the gene was not correlated with normal lymphocytes.…”

Section: Discussionmentioning

confidence: 99%

“…20) Human thymoma is a peculiar epithelial neoplasm in which abnormal epithelium supports the differentiation of polyclonal T-lymphocytes. [27][28][29][30] Immunophenotypic studies have shown that most lymphocytes in thymoma are identical to normal cortical thymocytes. [27][28][29] It has already been demonstrated by conventional histology that thymomas contain lymphocytes with a stimulated or activated appearance, and some mitoses are also seen.…”

Section: Discussionmentioning

confidence: 99%

“…[27][28][29][30] Immunophenotypic studies have shown that most lymphocytes in thymoma are identical to normal cortical thymocytes. [27][28][29] It has already been demonstrated by conventional histology that thymomas contain lymphocytes with a stimulated or activated appearance, and some mitoses are also seen. [27][28][29] However, in our study, neither mRNA nor protein level of GPI-80 in thymoma correlated with lymphocyte infiltration of thymoma.…”

Section: Discussionmentioning

confidence: 99%

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Glycosylphosphatidyl inositol‐anchored protein (GPI‐80) gene expression is correlated with human thymoma stage

et al. 2003

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Thymoma is one of the most common solid tumors in the mediastinum. Because there is no typical cell line for human thymoma, the development and use of molecular-based therapy for thymoma will require detailed molecular-genetic analysis of patients' tissues. Recent reports showed that genetic aberrations in thymoma were most frequently seen in chromosome 6q regions. We investigated the use of oligonucleotide arrays to monitor in vivo expression levels of genes in chromosome 6 regions in early- hymoma is a neoplasm of thymic epithelial cells mixed with lymphocytes. Although thymomas are usually encapsulated, some are characterized by locally invasive growth, pleural dissemination, or extrathoracic metastasis. However, there seems to be no cytological difference between noninvasive thymoma and invasive/metastatic thymoma.1-3) Several approaches have been made to differentiate non-invasive thymoma from invasive/metastatic thymoma, but the issue still remains controversial. Malignancy is generally evaluated in terms of the tumor's macroscopic invasiveness seen at the time of surgery.The elucidation of the genetic events underlying the initiation and progression of human thymoma has been hampered by limitations inherent in both in vitro and in vivo methods of study. The most significant limitation of in vitro-based systems is that there is no typical cell line for human thymoma. Also, genetic information derived from cell lines may not accurately reflect the molecular events taking place in the tissues from which they were derived. Examining tumors for alterations in gene expression is a potentially useful approach to identifying molecular differences between early-and late-stage thymomas. The advent of high-density oligonucleotide microarray technology, 4) with its capacity for simultaneous monitoring of thousands of genes, provides a unique opportunity for high-throughput genetic analysis of tumors. [5][6][7][8][9][10][11][12]

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Heterogeneity of Epithelial Cells and Reactive Components in Thymomas: An Ultrastructural and Immunohistochemical Study

Cited by 27 publications

References 26 publications

Arg and DAP3 expression was correlated with human thymoma stage

Arg and DAP3 expression was correlated with human thymoma stage

PE-35-related antigen expression and CD1a-positive lymphocytes in thymoma subtypes based on Müller-Hermelink classification

Glycosylphosphatidyl inositol‐anchored protein (GPI‐80) gene expression is correlated with human thymoma stage

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