Heterogeneity of beta-conglycinin

Thanh, Vu Huu; Shibasaki, Kazuo

doi:10.1016/0005-2795(76)90068-4

Cited by 129 publications

(84 citation statements)

References 21 publications

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“…The rx'-polypeptide was most abundant the main isomer and minor ones comprised equal amounts of rx and rx'. This finding is inconsistent with previous reports, 8 • 16 > and suggests that the isomers in the Hypo are different from those in the cotyledon in their polypeptide constituents. The isomers would have to be purified before their components could be identified.…”

contrasting

confidence: 99%

Components of Storage Protein in Hypocotyl-radicle Axis of Soybean (Glycine max. cv. Enrei) Seeds

Sugimoto¹,

Momma²,

Hashizume³

et al. 1987

Agricultural and Biological Chemistry

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contrasting

confidence: 99%

Components of Storage Protein in Hypocotyl-radicle Axis of Soybean (Glycine max. cv. Enrei) Seeds

Sugimoto¹,

Momma²,

Hashizume³

et al. 1987

Agricultural and Biological Chemistry

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“…The storage proteins of soybean seeds are comprised of two multimeric globulins, namely 7S and 11S globulins. The 7S globulin consists of b-conglycinin subunits and the 11S consists of glycinin proteins (Hill and Breidenbach, 1974;Thanh and Shibasaki, 1976). From a total of 17 transcripts for seed storage proteins, only two showed a varied pattern compared to transcripts for other seed storage proteins.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis Reveals a Critical Role ofCHS7andCHS8Genes for Isoflavonoid Synthesis in Soybean Seeds

et al. 2006

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We have used cDNA microarray analysis to examine changes in gene expression during embryo development in soybean (Glycine max) and to compare gene expression profiles of two soybean cultivars that differ in seed isoflavonoid content. The analysis identified 5,910 genes that were differentially expressed in both soybean cultivars grown at two different locations for two consecutive years in one of the five different stages of embryo development. An ANOVA analysis with P value , 0.05 and , 0.01 indicated that gene expression changes due to environmental factors are greater than those due to cultivar differences. Most changes in gene expression occurred at the stages when the embryos were at 30 or 70 d after pollination. A significantly larger fraction of genes (48.5%) was expressed throughout the development and showed little or no change in expression. Transcript accumulation for genes related to the biosynthesis of storage components in soybean embryos showed several unique temporal expressions. Expression patterns of several genes involved in isoflavonoid biosynthesis, such as Phenylalanine Ammonia-Lyase, Chalcone Synthase (CHS) 7, CHS8, and Isoflavone Synthase2, were higher at 70 d after pollination in both the cultivars. Thus, expression of these genes coincides with the onset of accumulation of isoflavonoids in the embryos. A comparative analysis of genes involved in isoflavonoid biosynthesis in RCAT Angora (high seed isoflavonoid cultivar) and Harovinton (low seed isoflavonoid cultivar) revealed that CHS7 and CHS8 were expressed at significantly greater level in RCAT Angora than in Harovinton. Our study provides a detailed transcriptome profiling of soybean embryos during development and indicates that differences in the level of seed isoflavonoids between these two cultivars could be as a result of differential expression of CHS7 and CHS8 during late stages of seed development.

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“…The individual subunits can be resolved from one another by ion exchange chromatography after denaturation of the native complex with urea (Thanh & Shibasaki 1976a) or by sodium dodecyl sulphate (SDS) polyacrylamide gel electrophoresis (PAGE). Based on the electrophoretic mobility of the second method, the molecular weight of the ∀', ∀ and ∃ subunits were estimated at 57, 57, and 42 kDa, respectively (Thanh & Shibasaki 1976b).…”

Section: ∃-Conglycinin (7s Fraction)mentioning

confidence: 99%

“…Among the ten theoretically possible multiple forms, six (B 1 to B 6 ) have been demonstrated to occur (Thanh & Shibasaki 1976b). The subunit compositions of the six components are as follows: B 1 is composed of 1 ∀' and 2 ∃; B 2 is composed of 1 ∀ and 2 ∃; B 3 is composed of 1 ∀ , 1 ∀' and 1 ∃; B 4 is composed of 2 ∀ and 1 ∃; B 5 is composed of 2 ∀ and 1 ∀' and B 6 is composed of 3 ∀.…”

Section: ∃-Conglycinin (7s Fraction)mentioning

confidence: 99%

Developments in plant breeding for improved nutritional quality of soya beans I. Protein and amino acid content

Clarke

Wiseman

2000

J. Agric. Sci.

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Soya beans, like other legumes, contain low concentrations of the nutritionally essential sulphur amino acid, methionine. Cysteine, although not an essential amino acid because it can be synthesized from methionine, also influences the nutritional quality of soya bean products when it is only present in low levels. A low cysteine content will also aggravate a methionine deficiency. Soya bean lines deficient in 7S protein subunits have been identified. The 7S proteins contain substantially less methionine and cysteine than the 11S proteins. With the myriad of genetic null alleles for these subunits it may be possible to tailor the 7S/11S storage protein ratio and their total composition in seeds to include only those subunits with the richest sulphur amino acid composition. Cotyledon feeding experiments, using isolated soya bean cotyledons, demonstrated that addition of methionine to the culture media caused increased synthesis of both proteins and free amino acids but the mechanism by which this takes place is not clear. Biotechnological approaches to improve nutritional value of soya beans include elevated expression of genes that originate from other species which encode high-sulphur proteins. High level expression of a 2S Brazil nut albumin gene in soya bean resulted in raised methionine concentration although the Brazil nut gene is highly antigenic and therefore will not be useful in production agriculture. Modification of glycinin to increase sulphur amino acid content is possible, and these gene products are capable of normal assembly into trimers in vitro although are rapidly degraded in vivo by the asparaginyl endopeptidase responsible for post-translational modification of proglycinin. Solutions to the methionine deficiency may be anticipated from a combination of approaches followed in laboratories worldwide. Many of these approaches are not without difficulty but, despite this, the likelihood is that soya beans with improved nutritional quality (which may not be confined to sulphur-containing amino acids as other nutritionally essential amino acids are also valuable) will be available in the near future. It will be essential to confirm that the increased total methionine (or other amino acid) is digestible to the animal to at least the same degree as conventional cultivars.

show abstract

Heterogeneity of beta-conglycinin

Cited by 129 publications

References 21 publications

Components of Storage Protein in Hypocotyl-radicle Axis of Soybean (Glycine max. cv. Enrei) Seeds

Components of Storage Protein in Hypocotyl-radicle Axis of Soybean (Glycine max. cv. Enrei) Seeds

Transcriptome Analysis Reveals a Critical Role ofCHS7andCHS8Genes for Isoflavonoid Synthesis in Soybean Seeds

Developments in plant breeding for improved nutritional quality of soya beans I. Protein and amino acid content

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