Heterogeneity of Benefit from Earlier Time-to-Antibiotics for Sepsis

Hechtman, Rachel K.; Kipnis, Patricia; Cano, Jennifer; Seelye, Sarah; Liu, Vincent X.; Prescott, Hallie C.

doi:10.1164/rccm.202310-1800oc

Cited by 3 publications

(1 citation statement)

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“…For patients with sepsis, it is well established that survival is directly linked to the prompt initiation of effective antibiotic therapy [ 15 , 16 ]. It would therefore be desirable to identify a reliable method for direct testing of positive blood cultures that can establish susceptibility or resistance to colistin.…”

Section: Introductionmentioning

confidence: 99%

The Use of CHROMID® Colistin R for the Detection of Colistin-Resistant Gram-Negative Bacteria in Positive Blood Cultures

Marrs,

Milburn,

Eltringham

et al. 2024

Antibiotics

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The aim of this study was to assess the utility of CHROMID® Colistin R for direct detection of colistin-resistant Gram-negative bacteria from positive blood cultures. A total of 390 blood cultures from hospitalised patients containing Gram-negative bacteria were included in this study. These blood cultures were referred to clinical laboratories in the United Kingdom and Türkiye. A further 16 simulated positive blood culture bottles were included that contained a range of colistin-resistant strains as well as susceptible control strains. Fluid from each positive blood culture was diluted 1/200 in saline and 10 µL aliquots cultured onto cystine-lactose-electrolyte-deficient agar and CHROMID® Colistin R. All recovered bacteria were identified, and for Gram-negative bacteria, their minimum inhibitory concentration of colistin was measured using the broth microdilution method. From a total of 443 Gram-negative isolates, 57 colistin-resistant isolates were recovered, of which 53 (93%) grew on CHROMID® Colistin R within 18 h. Of the 377 isolates determined to be colistin-susceptible, only 9 isolates were able to grow, including 6 isolates of Pseudomonas aeruginosa. For positive blood cultures that are shown to contain Gram-negative bacteria, culture on CHROMID® Colistin R is a useful diagnostic tool to detect susceptibility or resistance to colistin within 18 h.

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Section: Introductionmentioning

confidence: 99%