Heterogeneity of a major allergen from olive (Olea europea) pollen

Wheeler, A. W.; Hickman, B.E.; Fox, B.

doi:10.1016/0161-5890(90)90005-k

Cited by 44 publications

(33 citation statements)

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“…But the allergenic pattern of olive also displays polypeptides of higher molecular mass that have not been explored yet. IgE reactive proteins of 42, 45-47, 60 -65, and 70 kDa have been detected in the pollen extract (12, 14 -17), and some of them seem likely to be of high clinical significance close to that of Ole e 1, the main allergen from olive pollen, to which Ͼ70% olive allergic patients are sensitive as deduced from the prevalence observed in immunoblotting assays (6,15,16).…”

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confidence: 99%

Ole e 9, a Major Olive Pollen Allergen Is a 1,3-β-Glucanase

Huecas

Villalba

Rodrı́guez

2001

Journal of Biological Chemistry

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Olive pollen allergy is a clinical disorder affecting the human population of Mediterranean areas. A novel major allergen, Ole e 9, has been isolated from olive pollen by gel permeation, hydrophobic affinity, and reversephase high performance liquid chromatographies. It is involved in the allergic responses of 65% of patients suffering olive pollinosis. Ole e 9 (molecular mass of 46.4 kDa) displays 1,3-␤-endoglucanase activity (38.9 ؎ 5.6 mg of glucose released/min ؋ mol of protein at pH 4.5-6.0 using laminarin as substrate). It is the first 1,3-␤-glucanase, a member of the "pathogenesis-related" protein family, detected in pollen tissue. Seven tryptic peptides of the allergen were sequenced by Edman degradation and used for designing primers to clone the cDNA codifying the protein. Specific cDNA for Ole e 9 was synthesized from total RNA and amplified using the polymerase chain reaction. The allergen sequence showed an open reading frame of 460 amino acids comprising a putative signal peptide of 26 residues. It shows 39, 33, and 32% sequence identity including the catalytic residues when compared with 1,3-␤-glucanases from wheat, willow, and Arabidopsis thaliana, respectively. Northern blot analysis showed that Ole e 9 transcript is specifically expressed in the pollen tissue, and highly conserved counterparts were only detected in taxonomically related pollens.

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confidence: 99%

Ole e 9, a Major Olive Pollen Allergen Is a 1,3-β-Glucanase

Huecas

Villalba

Rodrı́guez

2001

Journal of Biological Chemistry

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“…This is supported by the existence of a correlation between the abundance of olive trees in the patients’ neighborhood and the incidence of skin-tested sensitivity among individuals with suspected atopic respiratory allergy from a population of Israelis [68]. These facts could help to explain the differences found by several authors among allergenic patterns as well as for the prevalence of olive allergens [15, 18, 19, 21], since they would depend on the particular sera used in each study, besides the specific technique employed for the analysis. As an example, the prevalence of Ole e 6 was found to range from 5 to 20% depending on the geographical area of sensitization of the patients suffering of olive pollinosis.…”

Section: Geographic Dependence On the Prevalence Of Olive Allergensmentioning

confidence: 92%

“…Early studies showed the presence of 20 fractions with allergenic activity [11]. Furthermore, several authors have reported allergenic patterns of olive pollen extracts by using individual sera, all of them showing the existence of a large number of IgE-reactive protein bands [15, 18, 19, 20, 21, 22, 23]. After the identification of Ole e 1, and over the past 5 years, an increasing number of olive pollen allergens have been purified and characterized, thus confirming former prospects.…”

Section: Complexity Of the Ige-binding Pattern Of Olive Pollen Extractsmentioning

confidence: 99%

“…Although the prevalence of Ole e 1 differs in different geographical areas, ranging from 55 to 90%, it has always been shown to be the most prevalent allergen of olive pollen extract [13, 15, 16, 25]. Moreover, removal of Ole e 1 from the whole extract led to the nearly total disappearance of the allergenic activity of the extract [26].…”

Section: Structural Properties and Functional Roles Of Olive Pollen Amentioning

confidence: 99%

“…Afterwards, proteins of 17, 19, 41–42 and 50–60 kD were identified as major allergens of olive pollen [13, 14, 15], although other bands of 8 and 60–70 kD were also detected as IgE-reactive molecules in pollen extracts [8, 14]. Some of these IgE-reactive proteins of high molecular mass have recently been identified as aggregates (multimeric forms) of a major allergen of 20 kD.…”

Section: Introductionmentioning

confidence: 99%

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The Spectrum of Olive Pollen Allergens

Rodrı́guez

Villalba

Monsalve

et al. 2001

Int Arch Allergy Immunol

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Olive pollen is one of the most important causes of seasonal respiratory allergy in Mediterranean countries, where this tree is intensely cultivated. Among the high number of protein allergens detected in this pollen, 8 – Ole e 1 to Ole e 8 – have been isolated and characterized. Ole e 1 is the most frequent sensitizing agent, affecting more than 70% of the patients suffering of olive pollinosis, although others, such as Ole e 4 and Ole e 7, have also been shown to be major allergens. In this context, the prevalence of many olive pollen allergens seems to be dependent on the geographical area where the sensitized patients live. Some of the olive allergens have been revealed as members of known protein families: profilin (Ole e 2), Ca²⁺-binding proteins (Ole e 3 and Ole e 8), superoxide dismutase (Ole e 5) and lipid transfer protein (Ole e 7). No biological function has been demonstrated for Ole e 1, whereas Ole e 4 and Ole e 6 are new proteins without homology to known sequences from databases. cDNAs encoding for Ole e 1, Ole e 3 and Ole e 8 have been overproduced in heterologous systems. The recombinant products were correctly folded and exhibited the functional activities of the natural allergens. In addition to the Oleaceae family, other species, such as Gramineae or Betulaceae, contain pollen allergens structurally or immunologically related to those of the olive tree. This fact allows to detect and evaluate antigenic cross-reactivities involving olive allergens. The aim of this research is the development of new diagnostic tools for olive pollinosis and new approaches to improve the classical immunotherapy.

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Immunocytochemical localization of allergenic protein (Ole e I) in the endoplasmic reticulum of the developing pollen grain of olive (Olea europaea L.)

Rodríguez-García

Fernández

Alché

1995

Planta

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Abstract.The monoclonal antibody OL-1 and transmission electron microscopy were used to locate immunologically the major allergen of olive pollen. Ole e I, during pollen grain development. Within the pollen grain, allergenic proteins are located in the cisternae of the rough endoplasmic reticulum. Our findings indicate that the synthesis of these proteins starts in the vegetative cytoplasm of olive pollen during the early maturation stage.

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Heterogeneity of a major allergen from olive (Olea europea) pollen

Cited by 44 publications

References 9 publications

Ole e 9, a Major Olive Pollen Allergen Is a 1,3-β-Glucanase

Ole e 9, a Major Olive Pollen Allergen Is a 1,3-β-Glucanase

The Spectrum of Olive Pollen Allergens

Immunocytochemical localization of allergenic protein (Ole e I) in the endoplasmic reticulum of the developing pollen grain of olive (Olea europaea L.)

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