Heterogeneity in Human Embryonic Stem Cells May Prevent Endodermal Guided Differentiation

Surajlata, Srabani Kar Konsam

doi:10.4172/2157-7633.1000255

Cited by 2 publications

(4 citation statements)

References 52 publications

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“…From this standpoint we can say that heterogeneity is key to success of lineage specific differentiation of human embryonic stem cells. Hore, 2015;Konsam Surajlata, 2015). b.…”

Section: Difference Between Cell Linesmentioning

confidence: 99%

“…These cells were derived from the inner cell mass (ICM) of grade III poor quality blastocysts that were not suitable for in vitro fertility treatment. Both the lines as claimed by JNCASR were pluripotent, and have been extensively characterized and cultured continuously for over 250 passages (Barbaric et al, 2011;Bilican et al, 2012;Bonig and Papayannopoulou, 2012;Garima Hore, 2015;Inamdar et al, 2011;Inamdar et al, 2009;Konsam Surajlata, 2015;Nestor et al, 2011;Rippon et al, 2006;Shetty and Inamdar, 2012;Venu et al, 2010).…”

Section: Expansion Of Human Embryonic Stem Cells (Bjnhem19 and Bjnhem20)mentioning

confidence: 99%

“…BJNhem embroid body formation in ultra-low attachment plates (GarimaHore, 2015;Konsam Surajlata, 2015).…”

mentioning

confidence: 99%

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Culture Methods and Choice of Cells Makes a Difference in Outcome of Tissue Engineering- a Perspective

2016

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This perspective reviews the effects of different tissue engineering techniques, for induced differentiation of human embryonic stem cells into pulmonary lineage specific cells, and validating cell based regenerative therapy to ameliorate pulmonary degeneration. The etiology of degeneration differs from disease to disease. Likewise, strategies to regenerate lost tissues in its heterogeneity, should also cater to the regeneration process. To customize the same, researchers ought to device strategies to repair, replace or regenerate, in the spatio-temporal format, healthy tissues in their heterogeneous multi-functional existence, such that when transplanted in vitro , they may find their niche and integrate seamlessly into the tissue system and manifest full-fledged functional competence in order to reverse the erstwhile effects of degeneration. Cell based lung repair or regeneration is unquestionably the most promising agenda of regenerative medicine. In one of our studies, human embryonic stem cell (hESC) line H7 was successfully differentiated into three non-ciliated alveolar epithelial cells. In another study, BJNhem19 and BJNhem20 stem cell lines were used to induce similar guided endodermal differentiation into pulmonary lineage cells. Both studies were done using similar protocols, where the hESCs were first plated on gamma irradiated mouse embryonic fibroblast (MEF) feeder cells and undifferentiated hESCs were taken through embroid body formation. They were then subjected to induction into lung specific cells, by defined growth factors in Small Airways Growth Medium (SAGM) and Bronchiolar Endothelial Growth Media (BEGM). The H7 cells were found to express, both intracellularly and on their surfaces, characteristic marker proteins. However, the BJNhem19 and BJNhem20 cells showed poor differential potential, and could not be successfully differentiated into lung-specific cells. This difference in differentiation potential may be due to several intrinsic limitations, like genetic heterogeneity, anomalous morphogen receptivity or dormant/ unresponsive cytoplasmic determinants. These observations indicate that the choice of cells may play an important factor in tissue engineering. This review addresses the different culture methods and conditions needed for guided differentiation of stem cells. This perspective also discusses culture conditions of mesenchymal stem cells from different origins and dendritic stem cells from bone marrow.

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“…From this standpoint we can say that heterogeneity is key to success of lineage specific differentiation of human embryonic stem cells. Hore, 2015;Konsam Surajlata, 2015). b.…”

Section: Difference Between Cell Linesmentioning

confidence: 99%

Section: Expansion Of Human Embryonic Stem Cells (Bjnhem19 and Bjnhem20)mentioning

confidence: 99%

See 1 more Smart Citation

Culture Methods and Choice of Cells Makes a Difference in Outcome of Tissue Engineering- a Perspective

2016

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Section: Expansion Of Human Embryonic Stem Cells (Bjnhem19 and Bjnhem20)mentioning

confidence: 99%

Culture Methods and Choice of Cells Makes a Difference in Outcome of Tissue Engineering - A Perspective

Kar

Mitra

Banerjee

2015

Biol syst Open Access

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This perspective reviews the effects of different tissue engineering techniques, for induced differentiation of human embryonic stem cells into pulmonary lineage specific cells, and validating cell based regenerative therapy to ameliorate pulmonary degeneration. The etiology of degeneration differs from disease to disease. Likewise, strategies to regenerate lost tissues in its heterogeneity, should also cater to the regeneration process. To customize the same, researchers ought to device strategies to repair, replace or regenerate, in the spatio-temporal format, healthy tissues in their heterogeneous multi-functional existence, such that when transplanted in vitro, they may find their niche and integrate seamlessly into the tissue system and manifest full-fledged functional competence in order to reverse the erstwhile effects of degeneration. Cell based lung repair or regeneration is unquestionably the most promising agenda of regenerative medicine.In one of our studies, human embryonic stem cell line H7 was successfully differentiated into three non-ciliated alveolar epithelial cells. In another study, BJNhem19 and BJNhem20 stem cell lines were used to induce similar guided endodermal differentiation into pulmonary lineage cells. Both studies were done using similar protocols, where the hESCs were first plated on gamma irriadiated MEF feeder cells and undifferentiated hESCs were taken through embroid body formation. They were then subjected to induction into lung specific cells, by defined growth factors in Small Airways Growth Medium and Bronchiolar Endothelial Growth Media. The H7 cells were found to express, both intracellularly and on their surfaces, characteristic marker proteins. However, the BJNhem19 and BJNhem20 cells showed poor differential potential, and could not be successfully differentiated into lung-specific cells. This difference in differentiation potential may be due to several intrinsic limitations, like genetic heterogeneity, anomalous morphogen receptivity or dormant/ unresponsive cytoplasmic determinants. These observations indicate that the choice of cells may play an important factor in tissue engineering. This review addresses the different culture methods and conditions needed for guided differentiation of stem cells.This perspective also discusses culture conditions of mesenchymal stem cells from different origins and dendritic stem cells from bone marrow.

show abstract

Heterogeneity in Human Embryonic Stem Cells May Prevent Endodermal Guided Differentiation

Cited by 2 publications

References 52 publications

Culture Methods and Choice of Cells Makes a Difference in Outcome of Tissue Engineering- a Perspective

Culture Methods and Choice of Cells Makes a Difference in Outcome of Tissue Engineering- a Perspective

Culture Methods and Choice of Cells Makes a Difference in Outcome of Tissue Engineering - A Perspective

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